Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LEO78_RS19010 Genome accession   NZ_CP084353
Coordinates   4175565..4177280 (+) Length   571 a.a.
NCBI ID   WP_180807586.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain 71317     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4170565..4182280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO78_RS18985 (LEO78_18985) pdhR 4171718..4172482 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  LEO78_RS18990 (LEO78_18990) ampD 4172758..4173330 (-) 573 WP_124251318.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LEO78_RS18995 (LEO78_18995) - 4173478..4173948 (+) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  LEO78_RS19000 (LEO78_19000) nadC 4173954..4174811 (+) 858 WP_226032466.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LEO78_RS19005 (LEO78_19005) - 4175134..4175574 (+) 441 WP_226032467.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LEO78_RS19010 (LEO78_19010) pilB 4175565..4177280 (+) 1716 WP_180807586.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  LEO78_RS19015 (LEO78_19015) pilC 4177396..4178637 (+) 1242 WP_226032468.1 type II secretion system F family protein Machinery gene
  LEO78_RS19020 (LEO78_19020) pilD 4178660..4179532 (+) 873 WP_226032469.1 A24 family peptidase Machinery gene
  LEO78_RS19025 (LEO78_19025) coaE 4179552..4180166 (+) 615 WP_226032470.1 dephospho-CoA kinase -
  LEO78_RS19030 (LEO78_19030) zapD 4180204..4180926 (+) 723 WP_024945431.1 cell division protein ZapD -
  LEO78_RS19035 (LEO78_19035) yacG 4180936..4181130 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  LEO78_RS19040 (LEO78_19040) mutT 4181191..4181604 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62880.40 Da        Isoelectric Point: 5.4342

>NTDB_id=610816 LEO78_RS19010 WP_180807586.1 4175565..4177280(+) (pilB) [Aeromonas hydrophila strain 71317]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMENDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=610816 LEO78_RS19010 WP_180807586.1 4175565..4177280(+) (pilB) [Aeromonas hydrophila strain 71317]
CTGCCCATAATGACATCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAAGCCAAGGCCCAGCGCAAGCCCTTTGTAACCTTTCTGATCGAAAACGAAATCCTCGACAGCA
AGGCACTGGCAGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAATACCTCAATCAGAAGCTGATTGAAAAACATCATGTTCTGCCCATCTATACCCAAGGGCACACCCTCTATATCGC
CATGTCGGATCCCACCAATGTGTCTGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAAGCGCTGCTGGTAG
AGGAAAACAAACTCACCACAGCCATCGGCAAGCTGATGGAGAACGATCAGGATGCCCTCGGCATGGAGGATATCGACGAG
TCCGAGATCTCCGAACTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAAAGCGTCAATACGGCCGACGATGACGCGCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGA
CCAAATACCGCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCGACTCCACCTGTCAATCTGGCCAACCGCTTC
TCGGCCCGCCTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTACCCCAGGATGGTCGCATCAAACTGAAGCT
GTCACGCAACAAGTCGATGGATATGCGGGTCAATACCCTACCCACCATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCG
ACTCCTCCGCCGCACGCCTCAACATTGAGCAGCTCGGTTTCGACGATCGCCAGAAGGCGCAATATCTGCGTGCCCTGTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCGCTCTATACCGGTCTCAATATTCT
CAATACCACCGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCA
ATCCCAAGGCAGGGCTGACCTTCGCCAGTGCCCTGCGCTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAA
ATCCGGGATCTGGAGACCGCAGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTACTCTCCACTCTGCACACCAA
CTCGGCGGCGGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCA
TGGCTCAGCGGCTGGCGCGTAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTGCCCGAGGCAGAACTGCTTGAGCTG
GGTTTTACCCAGCAGCAGCTGGCCGCAGGTTTGCGGCTGTTCAAACCAGTTGGCTGCAAAGAGTGCTCGGGTGGGTACAA
GGGCCGGGTCGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGC
AAATTGCCGCCATCGCCCAGAAGGAAGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACC
AGTCTGGCGGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

100

0.557
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.064

99.124

0.536
  pilB Vibrio cholerae strain A1552

53.982

98.949

0.534
  pilB Vibrio campbellii strain DS40M4

51.943

99.124

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.424

0.506
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

96.848

0.377
  pilF Thermus thermophilus HB27

40.152

92.469

0.371