Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LEO81_RS13430 Genome accession   NZ_CP084351
Coordinates   2988485..2989624 (+) Length   379 a.a.
NCBI ID   WP_017766703.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain 71431     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2983485..2994624
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEO81_RS13400 (LEO81_13400) - 2983667..2984047 (-) 381 WP_010633544.1 diacylglycerol kinase -
  LEO81_RS13405 (LEO81_13405) - 2984204..2985265 (+) 1062 WP_226013953.1 3-deoxy-7-phosphoheptulonate synthase -
  LEO81_RS13410 (LEO81_13410) - 2985345..2986130 (-) 786 WP_226013954.1 ATP-binding cassette domain-containing protein -
  LEO81_RS13415 (LEO81_13415) btuC 2986117..2987124 (-) 1008 WP_226013955.1 vitamin B12 ABC transporter permease BtuC -
  LEO81_RS13420 (LEO81_13420) - 2987224..2987955 (-) 732 WP_226013956.1 ABC transporter substrate-binding protein -
  LEO81_RS13425 (LEO81_13425) - 2988069..2988425 (+) 357 WP_005302630.1 DMT family protein -
  LEO81_RS13430 (LEO81_13430) pilU 2988485..2989624 (+) 1140 WP_017766703.1 type IVa pilus ATPase TapW Machinery gene
  LEO81_RS13435 (LEO81_13435) glgC 2989882..2991153 (+) 1272 WP_201882352.1 glucose-1-phosphate adenylyltransferase -
  LEO81_RS13440 (LEO81_13440) - 2991289..2991768 (+) 480 WP_065017832.1 WbuC family cupin fold metalloprotein -
  LEO81_RS13445 (LEO81_13445) - 2991880..2992122 (-) 243 WP_005302637.1 FeoC-like transcriptional regulator -
  LEO81_RS13450 (LEO81_13450) feoB 2992119..2994392 (-) 2274 WP_201882360.1 Fe(2+) transporter permease subunit FeoB -
  LEO81_RS13455 (LEO81_13455) - 2994389..2994616 (-) 228 WP_019840265.1 FeoA family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42171.19 Da        Isoelectric Point: 6.2561

>NTDB_id=610761 LEO81_RS13430 WP_017766703.1 2988485..2989624(+) (pilU) [Aeromonas dhakensis strain 71431]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=610761 LEO81_RS13430 WP_017766703.1 2988485..2989624(+) (pilU) [Aeromonas dhakensis strain 71431]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAAAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATTTGAAACTGCCCACCGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGCT
CGGGCAAATCCACCTCGCTCGCGGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATCGGCGAAATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCGGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACTACTGATGGCGGCCGCCGGGCGGCGGTAGAGATCATGCTGGGCACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGACGAGGAGGAGGCAGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361