Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LDO52_RS01165 Genome accession   NZ_CP084300
Coordinates   254144..255856 (-) Length   570 a.a.
NCBI ID   WP_005094394.1    Uniprot ID   A0A2H9YRE2
Organism   Acinetobacter pseudolwoffii strain XMC5X702     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 249144..260856
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO52_RS01130 (LDO52_01130) - 249261..249584 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  LDO52_RS01135 (LDO52_01135) rlmB 249721..250470 (+) 750 WP_005168959.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LDO52_RS01140 (LDO52_01140) - 250477..251400 (+) 924 WP_005094400.1 DMT family transporter -
  LDO52_RS01145 (LDO52_01145) coaE 251422..252021 (-) 600 WP_100329415.1 dephospho-CoA kinase -
  LDO52_RS16380 pilD 252023..252883 (-) 861 WP_074383650.1 prepilin peptidase Machinery gene
  LDO52_RS01160 (LDO52_01160) pilC 252883..254112 (-) 1230 WP_100329414.1 type II secretion system F family protein Machinery gene
  LDO52_RS01165 (LDO52_01165) pilB 254144..255856 (-) 1713 WP_005094394.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LDO52_RS01170 (LDO52_01170) tpiA 256134..256925 (+) 792 WP_005094392.1 triose-phosphate isomerase -
  LDO52_RS01175 (LDO52_01175) secG 256939..257268 (+) 330 WP_005094388.1 preprotein translocase subunit SecG -
  LDO52_RS01205 (LDO52_01205) rimP 258444..258968 (+) 525 WP_005094386.1 ribosome maturation factor RimP -
  LDO52_RS01210 (LDO52_01210) nusA 259003..260487 (+) 1485 WP_005094384.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63731.98 Da        Isoelectric Point: 4.7621

>NTDB_id=610503 LDO52_RS01165 WP_005094394.1 254144..255856(-) (pilB) [Acinetobacter pseudolwoffii strain XMC5X702]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLSPTVIAETISVEFGEPLFDISAYDPAQI
LRDIIDEKLITKHRILPIFKNSSILYIAISNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDFDSSDPSKEEDEKDKGEEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLATRLSS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGF
TEADLQQPEFQIYEPVGCNECREGYKGRVGVYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=610503 LDO52_RS01165 WP_005094394.1 254144..255856(-) (pilB) [Acinetobacter pseudolwoffii strain XMC5X702]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCTTTGGCACATGCCAAGCAAGAAAAAATAGATATTGTGGCGGAGCTGATTAACCAGCAACAGCTCTCCC
CAACCGTTATTGCTGAAACGATTTCAGTCGAATTTGGTGAGCCGCTGTTTGATATCAGTGCCTATGATCCTGCGCAAATT
TTGCGGGATATCATTGATGAGAAGCTGATTACCAAGCATCGCATCCTGCCAATTTTTAAAAATTCCAGTATTTTATATAT
TGCCATCAGTAATCCGACCAATATTGAAGCGATTGATGCCGTTCGCTTTTCAACCAAGCTCAATATTGAAACCATTATTG
TTGAACACAATAAACTGGAAAAACTGATCGAGCAGAATTTTACCGAAGAAAGCACTTTTGAGTTTGATGAAGACTTTGAT
CTGGATGTAGATTTCGATTCATCTGATCCGAGTAAAGAAGAAGATGAAAAAGACAAAGGTGAAGAAGAAGCACCGATTGT
CAAATATATCAATAAATTATTAATCGATGCGATTCGTATGGGTGCTTCGGATCTGCATTTTGAACCTTATGAAAAAATCT
ACCGGGTTCGTTATCGGGTCGATGGGGTATTACGCCAAATTGCAACACCGCCGCTGCAGTTGGCAACCCGCCTGTCTTCA
CGTTTAAAAGTCATGTCGCAAATGGACATTTCTGAAAAACGTGTTCCGCAAGATGGCCGTATCAAGCTGAAACTCTCAAA
GAACAAAGCCATTGATTTCCGTGTCAACTCATTACCGACCCTGTTTGGCGAAAAACTGGTACTGCGTATTCTAGATCCAT
CTAGCGCGATGCTGGGGATCGATGCACTCGGTTATGAACCGGAACAAAAAGAACTGTTTATGCAGGCGCTGGATAAACCG
CAAGGCATGCTGCTGATTACCGGGCCAACGGGTTCTGGTAAAACGGTTTCACTTTATACCGGTTTGAACATCCTGAATCG
TGAAGATACCAATATTTCCACCGCGGAAGATCCGGTCGAGATCAACCTGCAAGGAATTAACCAGGTCAACGTCAACAATA
AAGTCGGCCTGACCTTCTCTGCTGCCTTGAAATCATTCTTACGTCAAGATCCAGATATCGTGATGGTCGGTGAGATCCGG
GATCTGGAAACTGCAGAAATTGCCATTAAAGCGGCACAGACCGGTCATATGGTGATGTCAACGCTGCATACCAACAGTGC
GCCTGAGACCCTGACCCGTTTGCGCAATATGGGCGTGCCGTCTTTTAATATTGCCACTTCAGTGAATCTGGTCATTGCAC
AACGACTGGCACGTCGCCTGTGTTCACAATGTAAAAAACCTGCCGACATTCCACAGCAGAGTTTATTGGAAATGGGTTTT
ACCGAAGCAGATCTCCAGCAACCAGAGTTCCAGATCTATGAGCCGGTAGGTTGCAATGAATGTCGCGAAGGCTATAAGGG
TCGTGTAGGTGTTTATGAAGTCATGAAAGTGACGCCTGAAATTTCTAGAATTATTATGGAAGATGGCAATGCACTTGAAA
TTGCCGATGCTTCTGCCCGTGCAGGTTTTAACAATTTACGCCGATCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCT
TTACAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2H9YRE2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.86

100

0.8
  pilB Acinetobacter baylyi ADP1

76.708

100

0.768
  pilB Legionella pneumophila strain ERS1305867

51.59

99.298

0.512
  pilF Neisseria gonorrhoeae MS11

49.11

98.596

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

48.474

97.719

0.474
  pilB Vibrio campbellii strain DS40M4

48.115

97.719

0.47
  pilB Vibrio cholerae strain A1552

50.192

91.228

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

34.91

100

0.375
  pilF Thermus thermophilus HB27

36.926

99.298

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.204

100

0.363