Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CYCME_RS11035 Genome accession   NC_021917
Coordinates   2301726..2302946 (+) Length   406 a.a.
NCBI ID   WP_015005194.1    Uniprot ID   S5TZX8
Organism   Cycloclasticus zancles 78-ME     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2296726..2307946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYCME_RS11020 (CYCME_2263) - 2297774..2298940 (-) 1167 WP_020933063.1 nucleotide sugar dehydrogenase -
  CYCME_RS11025 (CYCME_2264) - 2298940..2299713 (-) 774 WP_020933064.1 glycosyltransferase family 2 protein -
  CYCME_RS11030 (CYCME_2265) pilB 2300011..2301720 (+) 1710 WP_020933065.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CYCME_RS11035 (CYCME_2266) pilC 2301726..2302946 (+) 1221 WP_015005194.1 type II secretion system F family protein Machinery gene
  CYCME_RS11040 (CYCME_2267) pilD 2302976..2303851 (+) 876 WP_020933066.1 prepilin peptidase Machinery gene
  CYCME_RS11045 (CYCME_2268) coaE 2303858..2304463 (+) 606 WP_020933067.1 dephospho-CoA kinase -
  CYCME_RS11050 (CYCME_2269) zapD 2304504..2305271 (+) 768 WP_020933068.1 cell division protein ZapD -
  CYCME_RS11055 (CYCME_2270) yacG 2305273..2305461 (+) 189 WP_020933069.1 DNA gyrase inhibitor YacG -
  CYCME_RS11060 (CYCME_2271) - 2305472..2306407 (-) 936 WP_020933070.1 Nudix family hydrolase -
  CYCME_RS11065 (CYCME_2272) argJ 2306435..2307655 (-) 1221 WP_020161771.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44340.29 Da        Isoelectric Point: 7.3558

>NTDB_id=60899 CYCME_RS11035 WP_015005194.1 2301726..2302946(+) (pilC) [Cycloclasticus zancles 78-ME]
MASSASKITYIWEGTDNKGNRSKGEINARSDALVKAELRRQGIRPLKVKKKPQALFGSMGQKPIVPKDIAVFSRQLATMM
ASGIPLVQSFDIIGKGHEKPAMQDMLANIKSDIEGGSTLAESLGKYPFQFDELFCNLVEAGEHAGILEDLLVKIAEYKEK
TESLKAKIKKALTYPISVLVVAVVVTAILLIFVVPQFESLFSGFGADLPAFTQMVVELSRFMQEWWWAVFGIIGGVVFVL
MQVKKRSRKFNHLLDRMILKMPIIGDIMNKAAIARYARTLSTMSAAGVPLVEALESVSGATGNIVYSEAVLQMREQVATG
IQLQQAMKNVGLFPHMVVQMVAIGEEAGSVESMLGKVADFYEEEVDNAVDSLSSLIEPLIMAILGILVGGLIVAMYLPIF
MLGSVF

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=60899 CYCME_RS11035 WP_015005194.1 2301726..2302946(+) (pilC) [Cycloclasticus zancles 78-ME]
ATGGCATCTAGTGCATCAAAAATTACCTATATTTGGGAAGGAACCGACAATAAAGGCAACCGCAGCAAAGGGGAAATAAA
TGCCCGAAGTGATGCCCTTGTTAAAGCAGAACTGAGGCGTCAAGGGATTCGCCCTCTTAAGGTTAAGAAAAAGCCTCAAG
CACTTTTTGGTAGCATGGGTCAAAAACCAATTGTTCCTAAAGATATCGCGGTTTTCAGCCGTCAATTAGCAACCATGATG
GCGTCCGGCATTCCATTAGTACAATCTTTTGATATTATTGGCAAAGGACACGAAAAGCCTGCCATGCAAGATATGCTTGC
CAACATTAAATCAGACATTGAGGGCGGGTCAACACTCGCTGAAAGTTTAGGAAAATACCCTTTTCAATTCGATGAGCTAT
TTTGTAATTTAGTAGAAGCTGGCGAACATGCCGGTATTCTCGAAGATTTACTAGTCAAAATTGCTGAATATAAAGAAAAA
ACAGAAAGCTTAAAAGCTAAAATCAAAAAAGCCCTTACCTATCCTATTTCAGTATTAGTAGTGGCCGTTGTTGTTACCGC
AATCTTATTGATTTTTGTTGTGCCTCAGTTTGAAAGCTTATTTAGCGGCTTTGGTGCAGATTTACCTGCGTTTACACAAA
TGGTCGTCGAACTGTCTCGTTTTATGCAAGAGTGGTGGTGGGCTGTCTTTGGCATAATAGGCGGTGTTGTATTTGTTCTA
ATGCAAGTTAAAAAACGTTCACGTAAATTTAACCACCTGCTCGATAGAATGATACTGAAAATGCCAATTATTGGCGACAT
CATGAACAAAGCAGCCATTGCTCGTTATGCTAGAACGTTGTCCACCATGTCTGCTGCTGGCGTACCCTTAGTAGAAGCAC
TAGAGTCTGTTTCAGGGGCAACCGGAAACATTGTTTACTCTGAAGCCGTTTTACAAATGAGAGAACAAGTGGCGACCGGC
ATCCAATTACAACAAGCCATGAAAAATGTAGGCCTTTTCCCTCATATGGTTGTGCAAATGGTCGCTATTGGCGAAGAAGC
AGGCTCGGTCGAAAGCATGCTTGGTAAAGTGGCTGATTTTTATGAAGAAGAGGTCGACAACGCTGTGGATTCCTTATCTA
GCCTTATTGAACCCTTAATTATGGCTATTCTCGGTATCTTAGTGGGTGGTTTAATTGTTGCCATGTACCTGCCTATCTTT
ATGTTAGGGTCTGTTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(277-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S5TZX8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

60.945

99.015

0.603
  pilC Pseudomonas stutzeri DSM 10701

56.158

100

0.562
  pilC Acinetobacter baumannii D1279779

56.171

97.783

0.549
  pilC Acinetobacter baylyi ADP1

54.054

100

0.542
  pilG Neisseria meningitidis 44/76-A

43.829

97.783

0.429
  pilG Neisseria gonorrhoeae MS11

43.577

97.783

0.426
  pilC Vibrio cholerae strain A1552

41.709

98.03

0.409
  pilC Vibrio campbellii strain DS40M4

40.05

99.015

0.397

Multiple sequence alignment