Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CYCME_RS11030 Genome accession   NC_021917
Coordinates   2300011..2301720 (+) Length   569 a.a.
NCBI ID   WP_020933065.1    Uniprot ID   -
Organism   Cycloclasticus zancles 78-ME     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2295011..2306720
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CYCME_RS11005 (CYCME_2260) - 2295256..2296182 (-) 927 WP_020933060.1 glycosyltransferase -
  CYCME_RS11010 (CYCME_2261) - 2296182..2296418 (-) 237 WP_020933061.1 hypothetical protein -
  CYCME_RS11015 (CYCME_2262) - 2296422..2297753 (-) 1332 WP_020933062.1 flippase -
  CYCME_RS11020 (CYCME_2263) - 2297774..2298940 (-) 1167 WP_020933063.1 nucleotide sugar dehydrogenase -
  CYCME_RS11025 (CYCME_2264) - 2298940..2299713 (-) 774 WP_020933064.1 glycosyltransferase family 2 protein -
  CYCME_RS11030 (CYCME_2265) pilB 2300011..2301720 (+) 1710 WP_020933065.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CYCME_RS11035 (CYCME_2266) pilC 2301726..2302946 (+) 1221 WP_015005194.1 type II secretion system F family protein Machinery gene
  CYCME_RS11040 (CYCME_2267) pilD 2302976..2303851 (+) 876 WP_020933066.1 prepilin peptidase Machinery gene
  CYCME_RS11045 (CYCME_2268) coaE 2303858..2304463 (+) 606 WP_020933067.1 dephospho-CoA kinase -
  CYCME_RS11050 (CYCME_2269) zapD 2304504..2305271 (+) 768 WP_020933068.1 cell division protein ZapD -
  CYCME_RS11055 (CYCME_2270) yacG 2305273..2305461 (+) 189 WP_020933069.1 DNA gyrase inhibitor YacG -
  CYCME_RS11060 (CYCME_2271) - 2305472..2306407 (-) 936 WP_020933070.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63010.40 Da        Isoelectric Point: 4.8072

>NTDB_id=60898 CYCME_RS11030 WP_020933065.1 2300011..2301720(+) (pilB) [Cycloclasticus zancles 78-ME]
MAVSDEKFYLGGLARRLVLDGLITEDVALKYSEEAFKKKRPFVSYLVDQKVIDSQTIALSASEEFGVPLFDITALSKEVI
PKGVVDEKLIRKHHALPIFKRGNKLFVAVSDPTNLQALDEIKFQTRMSTEAILVEEDKLARVIDTAMDDEDTSMSDLLDG
DLDNIDFDDPDADPIDDIDSEIDDAPVVRFVNKILLDAIKKGASDIHFEPYEKNFRIRTRQDGILKEVASPPMNISNKLT
SRIKVMSRMNISERRIPQDGRIKMALSKKRAIDFRVNTCPTLYGEKIVLRILDPTSAQVGIEQLGFDETQKEAFLTAIKK
PYGMILVTGPTGSGKTVSLYTALNLLNDPNINISTAEDPVEITVSGINQVNVNPKTGLTFAEALRAFLRQDPDIIMVGEI
RDLETAEISVKAAQTGHLVLSTLHTNDAPQTLNRLNQMGVPPYNIAASVLLIMAQRLARKLCDKCKKEETLPTELLIKEG
FSPEEAEGLTTYKAVGCDHCTNGYKGRIGIFQVMPVSEAMENLIIKGGLTTELEEQAAQEGVLTLRESGFKKVIAGITSI
EEINRITKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=60898 CYCME_RS11030 WP_020933065.1 2300011..2301720(+) (pilB) [Cycloclasticus zancles 78-ME]
ATGGCTGTATCCGACGAAAAATTTTATTTAGGTGGCTTAGCAAGACGGTTGGTCTTAGACGGTCTAATTACTGAAGACGT
TGCACTCAAATATTCTGAGGAGGCTTTTAAAAAGAAAAGGCCTTTCGTAAGCTATCTTGTAGATCAAAAAGTTATAGACA
GCCAAACCATAGCTCTCTCTGCTTCTGAGGAGTTTGGCGTTCCATTGTTTGATATTACCGCCCTCAGCAAAGAGGTTATA
CCCAAAGGTGTCGTTGACGAAAAGCTAATTCGTAAACACCATGCATTACCAATATTTAAACGTGGTAATAAGTTATTTGT
TGCTGTTTCCGACCCAACCAATCTACAAGCCCTCGATGAAATAAAATTTCAAACGAGAATGAGCACTGAAGCCATTCTTG
TTGAAGAAGATAAATTAGCTCGCGTTATTGACACTGCGATGGATGATGAAGACACATCCATGAGTGATTTATTAGACGGC
GACCTAGATAATATCGATTTCGATGATCCTGATGCCGATCCCATCGATGATATTGACAGTGAAATTGATGATGCCCCCGT
TGTAAGGTTTGTTAACAAAATATTATTAGATGCTATTAAAAAAGGTGCGTCGGATATTCATTTTGAGCCGTATGAGAAAA
ACTTTAGGATCCGAACACGACAAGATGGCATCTTAAAAGAAGTAGCCTCCCCTCCGATGAACATCTCAAATAAGTTAACC
TCACGCATTAAGGTGATGTCACGGATGAATATTTCAGAACGCCGTATTCCACAGGACGGTCGTATTAAAATGGCTTTATC
GAAAAAAAGAGCCATCGACTTCCGTGTTAATACTTGCCCAACTCTATATGGTGAAAAAATCGTGCTGCGTATTTTAGACC
CAACCAGCGCACAAGTCGGTATTGAGCAACTTGGGTTTGATGAAACTCAAAAGGAAGCTTTCCTAACCGCTATCAAAAAG
CCATACGGCATGATTTTGGTGACAGGACCTACCGGTAGTGGTAAGACCGTTAGCCTTTATACCGCGCTTAACTTATTAAA
CGACCCAAACATCAATATTTCAACAGCTGAAGACCCGGTTGAAATTACTGTATCGGGTATTAACCAAGTCAACGTAAACC
CAAAAACTGGCTTAACCTTTGCTGAAGCGCTTCGAGCTTTTTTACGCCAAGACCCGGATATAATCATGGTCGGTGAGATT
CGAGACCTTGAAACCGCTGAAATTTCTGTTAAAGCCGCACAAACCGGTCACCTTGTTCTATCTACATTACACACAAACGA
TGCCCCACAAACATTAAACCGTTTAAACCAAATGGGCGTTCCACCCTACAATATTGCGGCATCTGTGTTATTAATTATGG
CGCAACGTTTAGCAAGAAAACTATGCGATAAATGTAAAAAAGAAGAGACATTACCAACAGAGTTATTAATCAAAGAAGGC
TTTAGCCCTGAAGAAGCTGAAGGATTAACAACTTACAAGGCTGTAGGCTGTGATCATTGCACTAACGGCTATAAAGGGCG
TATTGGCATTTTCCAGGTAATGCCTGTTTCTGAAGCCATGGAAAACCTTATCATTAAAGGTGGTTTAACAACTGAACTTG
AAGAACAAGCTGCACAAGAGGGCGTTTTAACACTCAGGGAGTCTGGCTTTAAAAAGGTCATCGCTGGTATTACCAGCATA
GAAGAAATAAACCGAATCACTAAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.826

100

0.564
  pilB Acinetobacter baylyi ADP1

52.313

98.77

0.517
  pilB Acinetobacter baumannii D1279779

51.337

98.594

0.506
  pilF Neisseria gonorrhoeae MS11

48.754

98.77

0.482
  pilB Vibrio cholerae strain A1552

49.905

92.619

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

49.049

92.443

0.453
  pilB Vibrio campbellii strain DS40M4

47.909

92.443

0.443
  pilF Thermus thermophilus HB27

39.483

95.255

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

39.768

91.037

0.362

Multiple sequence alignment