Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LCH18_RS15485 Genome accession   NZ_CP083947
Coordinates   3195001..3196713 (+) Length   570 a.a.
NCBI ID   WP_183173595.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain CSUSB1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3190001..3201713
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH18_RS15440 (LCH18_15440) nusA 3190585..3192069 (-) 1485 WP_004692706.1 transcription termination factor NusA -
  LCH18_RS15445 (LCH18_15445) rimP 3192109..3192633 (-) 525 WP_004692708.1 ribosome maturation factor RimP -
  LCH18_RS15475 (LCH18_15475) secG 3193587..3193916 (-) 330 WP_004692710.1 preprotein translocase subunit SecG -
  LCH18_RS15480 (LCH18_15480) tpiA 3193930..3194721 (-) 792 WP_010325769.1 triose-phosphate isomerase -
  LCH18_RS15485 (LCH18_15485) pilB 3195001..3196713 (+) 1713 WP_183173595.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LCH18_RS15490 (LCH18_15490) pilC 3196744..3197970 (+) 1227 WP_010325767.1 type II secretion system F family protein Machinery gene
  LCH18_RS15495 (LCH18_15495) pilD 3197970..3198830 (+) 861 WP_224467628.1 prepilin peptidase Machinery gene
  LCH18_RS15500 (LCH18_15500) coaE 3198837..3199436 (+) 600 WP_178895931.1 dephospho-CoA kinase -
  LCH18_RS15505 (LCH18_15505) - 3199450..3200367 (-) 918 WP_224467629.1 DMT family transporter -
  LCH18_RS15510 (LCH18_15510) rlmB 3200400..3201149 (-) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LCH18_RS15515 (LCH18_15515) - 3201293..3201616 (-) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63623.95 Da        Isoelectric Point: 5.0082

>NTDB_id=608771 LCH18_RS15485 WP_183173595.1 3195001..3196713(+) (pilB) [Acinetobacter johnsonii strain CSUSB1]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENHKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=608771 LCH18_RS15485 WP_183173595.1 3195001..3196713(+) (pilB) [Acinetobacter johnsonii strain CSUSB1]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTGATTGAAAATCATAAACTCTCCC
CACAAATGATCGCTGAAACGATTTCTTTAGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCTGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTTATCACCAAGCATCGTGTATTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCTACTAGCAATCCAACCAACATGGATGCGATGGACGCGATTCGCTTCAATTCTAAAATGAATATTGAAGCGATTATTG
TTGAACATCCTCAACTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCAAATGACGAAGAAGATGACTCACCTCAAGGGGATGAAGCGCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCT
CGTTTGAAAGTCATGTCACAAATGGACATTTCTGAAAAGCGCGTGCCACAAGATGGCCGGATTAAATTAAAGCTGTCGAA
AAGTAAAGCCATTGACTTTCGTGTCAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCCT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGACCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCAGAAGACCCCGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAACGTGAACAATA
AAGTCGGTCTTACCTTCTCTGCTGCGCTGAAGTCATTCTTACGTCAAGATCCTGATATTGTGATGGTGGGTGAGATTCGT
GACCTTGAAACAGCGGAAATCGCGATTAAAGCCGCGCAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTAATTGCAC
AACGTTTAGCACGCCGCTTATGTAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGTTTGCTCGAAATGGGCTTT
ACTTCAACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGATGTACTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.228

100

0.812
  pilB Acinetobacter baylyi ADP1

76.667

100

0.767
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.849

97.895

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.41

100

0.374
  pilF Thermus thermophilus HB27

36.602

100

0.367