Detailed information NTG0608743

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LCH18_RS01215
• Genome accession: NZ_CP083947
• Coordinates: 273089..274147 (+)
• Length: 352 a.a.
• NCBI ID: WP_058870420.1
• Uniprot ID: A0A345B4L2
• Organism: Acinetobacter johnsonii strain CSUSB1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 268089..279147

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCH18_RS01200 (LCH18_01200)	mraY	268369..269487 (+)	1119	WP_130235301.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
LCH18_RS01205 (LCH18_01205)	-	269557..270363 (+)	807	WP_224468119.1	putative RNA methyltransferase	-
LCH18_RS01210 (LCH18_01210)	ponA	270395..272923 (-)	2529	WP_224468121.1	penicillin-binding protein PBP1a	-
LCH18_RS01215 (LCH18_01215)	pilM	273089..274147 (+)	1059	WP_058870420.1	pilus assembly protein PilM	Machinery gene
LCH18_RS01220 (LCH18_01220)	pilN	274147..274803 (+)	657	WP_004691498.1	PilN domain-containing protein	Machinery gene
LCH18_RS01225 (LCH18_01225)	pilO	274800..275522 (+)	723	WP_004691497.1	type 4a pilus biogenesis protein PilO	Machinery gene
LCH18_RS01230 (LCH18_01230)	pilP	275522..276049 (+)	528	WP_094148569.1	pilus assembly protein PilP	Machinery gene
LCH18_RS01235 (LCH18_01235)	pilQ	276069..278132 (+)	2064	WP_224468122.1	type IV pilus secretin PilQ family protein	Machinery gene
LCH18_RS01240 (LCH18_01240)	-	278221..278703 (+)	483	WP_265088291.1	shikimate kinase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38953.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=608743 LCH18_RS01215 WP_058870420.1 273089..274147(+) (pilM) [Acinetobacter johnsonii strain CSUSB1]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARSEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=608743 LCH18_RS01215 WP_058870420.1 273089..274147(+) (pilM) [Acinetobacter johnsonii strain CSUSB1]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAATAATCGGTATTGGGTGGAGAGCTATGCACTAGTCCCATTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCAGTTGGTGATGCCCTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACGTCCATGGTGATTACTAAAATCATCGAAATGGATGCCGATATGAACGATGACGAACGTGAAATCCAGAT
TCGTGATGATGCTGAGCAATATATTCCATTCCCATTAGATGAAGCGAGTTTGGACTTTGAAGTCCTTCCAGACCGTTTAG
CCAATCCAAATCGTGTCAATGTACTCCTCGTTGCAACCCGCATTGAAAATGTAGAGGCCCGATCCGAAGCATTGGAGTTA
GGTGGTTTAACGCCTAAGATTGCCGATGTTGAAAGTTTCGCCATTGAAAATGCATTCAAAGTCTTTTCAGATACGCTTCC
AATGGGTGTAAACACCGTTGGGATTCTGGACATTGGTCACACCATGACGACCTTATCTGTCATGCAGAATAATAAAGTCA
TTTATACGCGTGAGCAGGTGTTCGGTGGTAAACAGCTGACTCAAGAAATTCAAAACCGTTATGGTTTATCTTTTGAAGAA
GCTGGACGTGCCAAAAAAAGCCGTACTTTGCCTGACGACTATGATATTGAAGTGCTTGAGCCTTTTCTTGAAGCTGTGGT
GCAACAAGCAGCACGTTCTTTGCAGTTCTTCTTTTCGTCCTCTCAATTCAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTCGCGAAGTTATTACAACAAAAATTAGGTTATCGGGTCACTATTGCCAACCCATTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A345B4L2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412