Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LCH17_RS09275 Genome accession   NZ_CP083944
Coordinates   2040644..2042350 (+) Length   568 a.a.
NCBI ID   WP_146061427.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain CSUSB2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2035644..2047350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCH17_RS09250 (LCH17_09250) pdhR 2036790..2037554 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  LCH17_RS09255 (LCH17_09255) ampD 2037830..2038402 (-) 573 WP_171279817.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LCH17_RS09260 (LCH17_09260) - 2038550..2039020 (+) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  LCH17_RS09265 (LCH17_09265) nadC 2039026..2039883 (+) 858 WP_224481186.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LCH17_RS09270 (LCH17_09270) - 2040206..2040637 (+) 432 WP_041216993.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LCH17_RS09275 (LCH17_09275) pilB 2040644..2042350 (+) 1707 WP_146061427.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  LCH17_RS09280 (LCH17_09280) pilC 2042466..2043707 (+) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  LCH17_RS09285 (LCH17_09285) pilD 2043730..2044602 (+) 873 WP_224481187.1 A24 family peptidase Machinery gene
  LCH17_RS09290 (LCH17_09290) coaE 2044622..2045236 (+) 615 WP_049046581.1 dephospho-CoA kinase -
  LCH17_RS09295 (LCH17_09295) zapD 2045274..2045996 (+) 723 WP_162498668.1 cell division protein ZapD -
  LCH17_RS09300 (LCH17_09300) yacG 2046006..2046200 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  LCH17_RS09305 (LCH17_09305) mutT 2046261..2046674 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62481.87 Da        Isoelectric Point: 5.4342

>NTDB_id=608726 LCH17_RS09275 WP_146061427.1 2040644..2042350(+) (pilB) [Aeromonas hydrophila strain CSUSB2]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=608726 LCH17_RS09275 WP_146061427.1 2040644..2042350(+) (pilB) [Aeromonas hydrophila strain CSUSB2]
ATGACCTCCAGCCCGAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCCCAGCGCAAACCTTTTGTGACCTTTCTCATCGAAAACGAAATCCTCGATAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGTTGATTGAAAAACATCACGTCCTGCCCATCTACACTCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCTACCAATGTGTCGGCACTGGAGGACTTTGGCTTCAGCTTCGGGTTGCACACCGAGGCTCTGCTGGTCGAGGAGAACA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATT
TCCGAACTGGAGGTGTCGGATGAGGGGTCGCGACTCGACGAGAGCGTCAATACAGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGATAGCAACTCCACCGGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAACGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CTGCGCGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGGCAGAAGGCGCAATATCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTCTCACTCTATACCGGCCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCTGGCGTCAACCAGGTGCAGGTCAATCCCAAGG
CAGGACTGACCTTCGCGAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGACGTAGTCATGGTCGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGAGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCCGAGGCTGAACTGCTTGAGCTGGGATTTACC
CAGCAGCAGCTGGCCGCAGGCTTGAGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.246

98.944

0.507
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375