Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   JF634_RS04155 Genome accession   NZ_CP083930
Coordinates   842166..843440 (-) Length   424 a.a.
NCBI ID   WP_002641291.1    Uniprot ID   V9H9Q9
Organism   Simonsiella muelleri strain DSM 2579     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 837166..848440
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JF634_RS04125 (JF634_04125) - 837415..838827 (+) 1413 WP_002641285.1 dihydrolipoyl dehydrogenase -
  JF634_RS04130 (JF634_04130) - 838945..839151 (+) 207 WP_002641286.1 glycine zipper 2TM domain-containing protein -
  JF634_RS04135 (JF634_04135) - 839228..839845 (+) 618 WP_002641287.1 HAD family hydrolase -
  JF634_RS04140 (JF634_04140) zapD 839891..840640 (-) 750 WP_002641288.1 cell division protein ZapD -
  JF634_RS04145 (JF634_04145) coaE 840654..841259 (-) 606 WP_002641289.1 dephospho-CoA kinase -
  JF634_RS04150 (JF634_04150) pilD 841262..842164 (-) 903 WP_002641290.1 A24 family peptidase Machinery gene
  JF634_RS04155 (JF634_04155) pilG 842166..843440 (-) 1275 WP_002641291.1 type II secretion system F family protein Machinery gene
  JF634_RS04160 (JF634_04160) pilF 843540..845228 (-) 1689 WP_002641292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JF634_RS04165 (JF634_04165) - 845444..845887 (-) 444 WP_002641293.1 DUF4149 domain-containing protein -
  JF634_RS04170 (JF634_04170) greA 846019..846495 (-) 477 WP_002641294.1 transcription elongation factor GreA -
  JF634_RS04175 (JF634_04175) - 846699..847199 (-) 501 WP_002641295.1 hypothetical protein -
  JF634_RS04180 (JF634_04180) pssA 847398..848210 (-) 813 WP_002641296.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 46034.61 Da        Isoelectric Point: 10.1099

>NTDB_id=608558 JF634_RS04155 WP_002641291.1 842166..843440(-) (pilG) [Simonsiella muelleri strain DSM 2579]
MATLKKPVKKAPVKAKRPAAKPQKKERGNRYQFEGRNLQTESIVRGEVVARNEEEARQKLQRRQVKVLQIVKMKKAKNKK
ITSADIAVFTRQLCTMMKAGLPLMQAFDIVAKGHSNASMTNLLMSVRADVEQGTSMADAFSKHPKYFSKFYCNLVGAGEA
GGVLDSLLDKLATYMEKTEAIKKKVKSALTYPIAILVVAVVLVIVMMMFVLPEFKKVYDSMGAKLPPLTEFMMGISNAFV
AHGALIIIGLVGTVAGGIFIVKNSPALQKKLDALLLKLPIFGDIVMKATIARWSRTTATLFTAGVPLVEVLDSVAGASGN
IIYEEATNEIRSKVNQGISLTSGMQAAQIFPNMVVQMAAIGEEAGALDDMLNKVAEFYEEEVDTAVETLSSLMEPIIMVV
LGGIVGTILVAMYLPLFNLGQVVG

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=608558 JF634_RS04155 WP_002641291.1 842166..843440(-) (pilG) [Simonsiella muelleri strain DSM 2579]
ATGGCTACTCTAAAAAAACCTGTCAAAAAAGCACCCGTGAAAGCAAAAAGACCTGCTGCCAAACCACAAAAAAAAGAGCG
TGGTAATCGATATCAATTTGAAGGGCGAAATCTTCAAACGGAGAGTATTGTGCGCGGTGAGGTGGTTGCGCGTAATGAAG
AAGAAGCACGTCAGAAATTACAACGCCGTCAGGTAAAAGTCCTGCAAATTGTTAAAATGAAAAAGGCGAAAAATAAGAAA
ATTACTTCTGCTGATATTGCCGTTTTCACGCGCCAATTATGTACCATGATGAAGGCTGGTTTGCCATTAATGCAAGCTTT
TGATATTGTGGCAAAAGGGCATTCCAATGCGTCCATGACTAATTTGCTGATGTCGGTGCGTGCAGATGTGGAACAGGGCA
CGTCTATGGCAGACGCATTTTCTAAACACCCGAAATATTTCAGTAAATTTTATTGTAACCTAGTGGGTGCAGGTGAAGCG
GGTGGTGTATTGGATTCTTTGTTAGACAAATTAGCCACTTACATGGAAAAAACCGAAGCAATTAAGAAAAAAGTAAAATC
TGCTTTAACTTATCCAATTGCCATTTTGGTGGTGGCGGTGGTATTGGTTATTGTGATGATGATGTTCGTATTGCCAGAAT
TTAAAAAGGTATACGATAGCATGGGCGCAAAATTGCCACCATTGACTGAGTTTATGATGGGTATTTCCAATGCGTTTGTG
GCTCATGGTGCATTGATTATTATTGGTTTAGTAGGTACGGTTGCTGGTGGGATTTTTATTGTGAAAAACTCGCCTGCATT
ACAGAAGAAATTAGACGCTTTATTACTGAAATTGCCTATTTTTGGTGATATTGTGATGAAAGCAACCATTGCACGTTGGT
CGCGTACTACGGCCACGCTGTTTACGGCTGGCGTACCACTGGTGGAAGTATTGGATTCAGTGGCTGGTGCATCGGGTAAT
ATTATTTATGAAGAAGCGACTAATGAAATTCGTTCTAAAGTTAATCAGGGTATTTCTTTGACTTCAGGCATGCAGGCCGC
CCAAATTTTCCCCAATATGGTGGTGCAAATGGCGGCCATTGGTGAAGAGGCTGGTGCGCTTGATGATATGTTGAATAAAG
TGGCTGAATTCTATGAAGAAGAAGTAGATACAGCAGTGGAAACGTTGTCATCATTGATGGAACCCATCATCATGGTGGTA
TTGGGGGGTATTGTAGGGACTATTTTAGTGGCGATGTATCTGCCATTGTTCAACTTGGGTCAAGTGGTTGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(89-212)

Type II secretion system (T2SS), protein F (T2SSF)

(293-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V9H9Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

63

94.34

0.594
  pilG Neisseria meningitidis 44/76-A

62

94.34

0.585
  pilC Legionella pneumophila strain ERS1305867

48.492

93.868

0.455
  pilC Pseudomonas stutzeri DSM 10701

44.691

95.519

0.427
  pilC Acinetobacter baumannii D1279779

45.736

91.274

0.417
  pilC Acinetobacter baylyi ADP1

45.478

91.274

0.415
  pilC Vibrio cholerae strain A1552

38.903

94.575

0.368
  pilC Vibrio campbellii strain DS40M4

37.317

96.698

0.361