Detailed information NTG0608557

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: JF634_RS04150
• Genome accession: NZ_CP083930
• Coordinates: 841262..842164 (-)
• Length: 300 a.a.
• NCBI ID: WP_002641290.1
• Uniprot ID: V9HMH3
• Organism: Simonsiella muelleri strain DSM 2579
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 836262..847164

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JF634_RS04120 (JF634_04120)	-	836358..837107 (+)	750	WP_002641284.1	glutathione peroxidase	-
JF634_RS04125 (JF634_04125)	-	837415..838827 (+)	1413	WP_002641285.1	dihydrolipoyl dehydrogenase	-
JF634_RS04130 (JF634_04130)	-	838945..839151 (+)	207	WP_002641286.1	glycine zipper 2TM domain-containing protein	-
JF634_RS04135 (JF634_04135)	-	839228..839845 (+)	618	WP_002641287.1	HAD family hydrolase	-
JF634_RS04140 (JF634_04140)	zapD	839891..840640 (-)	750	WP_002641288.1	cell division protein ZapD	-
JF634_RS04145 (JF634_04145)	coaE	840654..841259 (-)	606	WP_002641289.1	dephospho-CoA kinase	-
JF634_RS04150 (JF634_04150)	pilD	841262..842164 (-)	903	WP_002641290.1	A24 family peptidase	Machinery gene
JF634_RS04155 (JF634_04155)	pilG	842166..843440 (-)	1275	WP_002641291.1	type II secretion system F family protein	Machinery gene
JF634_RS04160 (JF634_04160)	pilF	843540..845228 (-)	1689	WP_002641292.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JF634_RS04165 (JF634_04165)	-	845444..845887 (-)	444	WP_002641293.1	DUF4149 domain-containing protein	-
JF634_RS04170 (JF634_04170)	greA	846019..846495 (-)	477	WP_002641294.1	transcription elongation factor GreA	-

Sequence

Protein

• Length: 300 a.a.
• Molecular weight: 33636.15 Da
• Isoelectric Point: 8.9966

>NTDB_id=608557 JF634_RS04150 WP_002641290.1 841262..842164(-) (pilD) [Simonsiella muelleri strain DSM 2579]
MENMAIFMAVLFGLLVGSFLNVVIYRLPVMLNREWNEAAREQLKLDDEFIQTLPENIAQTLRQRIGLTPEQMGVFTLSRP
RSRCGNCGAPVRAWQNIPIISWLILRGKCHTCQTPISIRYPLVELLTGVLFGLVAWRYGWTEITVWGCLLTAMVIAMTFI
DADTQILPDDLTKPLVWLGLLFNWRMGFVSWQQALLGAVIGYMSLYLLNKIHVILRGIDGMGQGDFKLLAAIGAWVGVAN
LPIVVLMASVIGIVAALVKRVTKSQPMAFGPCLAIAGWFIFLFHQQATMAITWWLHKSGF

Nucleotide

• Length: 903 bp

>NTDB_id=608557 JF634_RS04150 WP_002641290.1 841262..842164(-) (pilD) [Simonsiella muelleri strain DSM 2579]
ATGGAGAATATGGCGATTTTTATGGCGGTATTGTTTGGGTTATTGGTTGGCAGCTTCCTGAACGTGGTGATTTACCGTCT
GCCCGTGATGCTCAATCGAGAGTGGAACGAGGCAGCGCGTGAACAACTTAAATTAGACGATGAATTTATCCAAACGCTGC
CTGAAAATATTGCCCAAACACTTCGCCAACGTATCGGCTTGACTCCTGAACAAATGGGCGTGTTTACCTTGTCGCGTCCA
CGCTCACGCTGTGGAAATTGTGGTGCACCTGTGCGAGCGTGGCAGAATATTCCAATTATTAGTTGGTTGATTTTGCGTGG
AAAATGTCATACTTGTCAAACGCCAATCAGTATTCGTTATCCTTTGGTAGAACTGCTGACGGGGGTGTTGTTTGGTTTAG
TGGCGTGGCGATACGGTTGGACGGAAATCACCGTGTGGGGCTGTTTACTCACAGCGATGGTTATTGCAATGACATTTATT
GACGCGGACACGCAAATTTTGCCTGATGATTTGACTAAACCTTTGGTTTGGTTGGGTTTGTTATTTAATTGGCGTATGGG
CTTTGTGTCGTGGCAACAAGCATTGTTGGGTGCTGTAATTGGTTATATGAGTTTGTATTTATTGAATAAAATTCATGTAA
TTTTGCGTGGCATAGATGGTATGGGGCAGGGCGATTTTAAATTATTGGCTGCGATTGGTGCGTGGGTTGGGGTGGCAAAC
TTGCCTATTGTGGTGTTGATGGCATCGGTTATTGGCATTGTGGCGGCGTTGGTGAAACGTGTTACCAAAAGTCAGCCTAT
GGCATTTGGTCCATGTTTGGCGATTGCTGGTTGGTTTATTTTTCTTTTTCATCAACAAGCTACAATGGCAATTACGTGGT
GGTTGCATAAATCTGGATTTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (149-256)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (11-138)

3D structure

• AlphaFold DB: V9HMH3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	53.559	98.333	0.527
pilD	Vibrio cholerae strain A1552	45.263	95	0.43
pilD	Acinetobacter nosocomialis M2	43.86	95	0.417
pilD	Vibrio campbellii strain DS40M4	43.357	95.333	0.413
pilD	Acinetobacter baumannii D1279779	43.509	95	0.413