Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NMD30_RS18700 Genome accession   NZ_CP100994
Coordinates   3788981..3790366 (+) Length   461 a.a.
NCBI ID   WP_240764977.1    Uniprot ID   -
Organism   Escherichia coli strain ET201     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3783981..3795366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD30_RS18675 (NMD30_18530) aroP 3784529..3785902 (+) 1374 WP_112826608.1 aromatic amino acid transporter AroP -
  NMD30_RS18680 (NMD30_18535) ampE 3785945..3786799 (-) 855 WP_000172008.1 beta-lactamase regulator AmpE -
  NMD30_RS18685 (NMD30_18540) ampD 3786796..3787347 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD30_RS18690 (NMD30_18545) nadC 3787435..3788328 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD30_RS18695 (NMD30_18550) pilA 3788531..3788971 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD30_RS18700 (NMD30_18555) pilB 3788981..3790366 (+) 1386 WP_240764977.1 type II secretion system protein GspE Machinery gene
  NMD30_RS18705 (NMD30_18560) hofC 3790356..3791558 (+) 1203 WP_000157259.1 protein transport protein HofC -
  NMD30_RS18710 (NMD30_18565) guaC 3791593..3792636 (-) 1044 WP_001217337.1 GMP reductase -
  NMD30_RS18715 (NMD30_18570) - 3792792..3792836 (-) 45 WP_120795372.1 protein YacM -
  NMD30_RS18720 (NMD30_18575) coaE 3792861..3793481 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  NMD30_RS18725 (NMD30_18580) zapD 3793481..3794224 (+) 744 WP_001194722.1 cell division protein ZapD -
  NMD30_RS18730 (NMD30_18585) yacG 3794234..3794431 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  NMD30_RS18735 (NMD30_18590) mutT 3794647..3795036 (-) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50651.36 Da        Isoelectric Point: 6.5396

>NTDB_id=608368 NMD30_RS18700 WP_240764977.1 3788981..3790366(+) (pilB) [Escherichia coli strain ET201]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDVPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCIHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=608368 NMD30_RS18700 WP_240764977.1 3788981..3790366(+) (pilB) [Escherichia coli strain ET201]
ATGAATATTCCACAGCTCACCGCCCTGTGTTTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTAGTCGATGTCCCTTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTGTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACTGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGGCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTATACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.478

83.948

0.382
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36