Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD30_RS18695 Genome accession   NZ_CP100994
Coordinates   3788531..3788971 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain ET201     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3783531..3793971
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD30_RS18675 (NMD30_18530) aroP 3784529..3785902 (+) 1374 WP_112826608.1 aromatic amino acid transporter AroP -
  NMD30_RS18680 (NMD30_18535) ampE 3785945..3786799 (-) 855 WP_000172008.1 beta-lactamase regulator AmpE -
  NMD30_RS18685 (NMD30_18540) ampD 3786796..3787347 (-) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD30_RS18690 (NMD30_18545) nadC 3787435..3788328 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD30_RS18695 (NMD30_18550) pilA 3788531..3788971 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NMD30_RS18700 (NMD30_18555) pilB 3788981..3790366 (+) 1386 WP_240764977.1 type II secretion system protein GspE Machinery gene
  NMD30_RS18705 (NMD30_18560) hofC 3790356..3791558 (+) 1203 WP_000157259.1 protein transport protein HofC -
  NMD30_RS18710 (NMD30_18565) guaC 3791593..3792636 (-) 1044 WP_001217337.1 GMP reductase -
  NMD30_RS18715 (NMD30_18570) - 3792792..3792836 (-) 45 WP_120795372.1 protein YacM -
  NMD30_RS18720 (NMD30_18575) coaE 3792861..3793481 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=608367 NMD30_RS18695 WP_000360904.1 3788531..3788971(+) (pilA) [Escherichia coli strain ET201]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=608367 NMD30_RS18695 WP_000360904.1 3788531..3788971(+) (pilA) [Escherichia coli strain ET201]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCAAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363