Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NMD37_RS19710 Genome accession   NZ_CP100966
Coordinates   4142480..4143865 (+) Length   461 a.a.
NCBI ID   WP_137481208.1    Uniprot ID   -
Organism   Escherichia coli strain ET350     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4137480..4148865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD37_RS19685 (NMD37_19590) aroP 4138028..4139401 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NMD37_RS19690 (NMD37_19595) ampE 4139444..4140298 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMD37_RS19695 (NMD37_19600) ampD 4140295..4140846 (-) 552 WP_097397835.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD37_RS19700 (NMD37_19605) nadC 4140934..4141827 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD37_RS19705 (NMD37_19610) pilA 4142030..4142470 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  NMD37_RS19710 (NMD37_19615) pilB 4142480..4143865 (+) 1386 WP_137481208.1 type II secretion system protein GspE Machinery gene
  NMD37_RS19715 (NMD37_19620) hofC 4143855..4145057 (+) 1203 WP_104773252.1 protein transport protein HofC -
  NMD37_RS19720 (NMD37_19625) guaC 4145092..4146135 (-) 1044 WP_001217338.1 GMP reductase -
  NMD37_RS19725 (NMD37_19630) - 4146291..4146335 (-) 45 WP_120795372.1 protein YacM -
  NMD37_RS19730 (NMD37_19635) coaE 4146360..4146980 (+) 621 WP_215260890.1 dephospho-CoA kinase -
  NMD37_RS19735 (NMD37_19640) zapD 4146980..4147723 (+) 744 WP_001194734.1 cell division protein ZapD -
  NMD37_RS19740 (NMD37_19645) yacG 4147733..4147930 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  NMD37_RS19745 (NMD37_19650) mutT 4148145..4148534 (-) 390 WP_000736013.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50637.27 Da        Isoelectric Point: 6.5405

>NTDB_id=607964 NMD37_RS19710 WP_137481208.1 4142480..4143865(+) (pilB) [Escherichia coli strain ET350]
MNIPQLTALCLRYQGVLLDTSEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=607964 NMD37_RS19710 WP_137481208.1 4142480..4143865(+) (pilB) [Escherichia coli strain ET350]
ATGAATATTCCACAGCTCACGGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATACCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CAGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGCCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCATTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.171

100

0.408
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36