Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD37_RS19705 Genome accession   NZ_CP100966
Coordinates   4142030..4142470 (+) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain ET350     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4137030..4147470
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD37_RS19685 (NMD37_19590) aroP 4138028..4139401 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  NMD37_RS19690 (NMD37_19595) ampE 4139444..4140298 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NMD37_RS19695 (NMD37_19600) ampD 4140295..4140846 (-) 552 WP_097397835.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD37_RS19700 (NMD37_19605) nadC 4140934..4141827 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD37_RS19705 (NMD37_19610) pilA 4142030..4142470 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  NMD37_RS19710 (NMD37_19615) pilB 4142480..4143865 (+) 1386 WP_137481208.1 type II secretion system protein GspE Machinery gene
  NMD37_RS19715 (NMD37_19620) hofC 4143855..4145057 (+) 1203 WP_104773252.1 protein transport protein HofC -
  NMD37_RS19720 (NMD37_19625) guaC 4145092..4146135 (-) 1044 WP_001217338.1 GMP reductase -
  NMD37_RS19725 (NMD37_19630) - 4146291..4146335 (-) 45 WP_120795372.1 protein YacM -
  NMD37_RS19730 (NMD37_19635) coaE 4146360..4146980 (+) 621 WP_215260890.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=607963 NMD37_RS19705 WP_000360901.1 4142030..4142470(+) (pilA) [Escherichia coli strain ET350]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=607963 NMD37_RS19705 WP_000360901.1 4142030..4142470(+) (pilA) [Escherichia coli strain ET350]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGGTTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTGAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363