Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K8374_RS22365 Genome accession   NZ_CP083746
Coordinates   4820897..4822183 (-) Length   428 a.a.
NCBI ID   WP_224457244.1    Uniprot ID   -
Organism   Pseudomonas sp. p1(2021b)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4815897..4827183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8374_RS22350 (K8374_22350) - 4817565..4819163 (-) 1599 WP_224457242.1 SPOR domain-containing protein -
  K8374_RS22355 (K8374_22355) aroB 4819179..4820276 (-) 1098 WP_224457243.1 3-dehydroquinate synthase -
  K8374_RS22360 (K8374_22360) aroK 4820374..4820892 (-) 519 WP_084853842.1 shikimate kinase AroK -
  K8374_RS22365 (K8374_22365) pilQ 4820897..4822183 (-) 1287 WP_224457244.1 type IV pilus secretin PilQ Machinery gene
  K8374_RS22370 (K8374_22370) - 4822089..4823105 (-) 1017 WP_224457245.1 pilus assembly protein PilP -
  K8374_RS22375 (K8374_22375) - 4823102..4823641 (-) 540 WP_224457246.1 PilN domain-containing protein -
  K8374_RS22380 (K8374_22380) pilM 4823638..4824498 (-) 861 WP_224457247.1 type IV pilus biogenesis protein PilM -
  K8374_RS22385 (K8374_22385) - 4824661..4827111 (+) 2451 WP_411969667.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 428 a.a.        Molecular weight: 46461.46 Da        Isoelectric Point: 5.2383

>NTDB_id=607621 K8374_RS22365 WP_224457244.1 4820897..4822183(-) (pilQ) [Pseudomonas sp. p1(2021b)]
MRSWMSVNGLGKWMLAILLTGQASMALASRNEPLSLNFQDIEVRAALQVLADFAGVNLVASDTVQGNLTLRLEGVPWEQA
LDLVLRSKGLARREEGNVLLVAPAAELAEQFREARLEQALDAQQQPMRRALLPIHHAKAADLAELLLAALADDGILTGRG
SLSVDARTNTLVVYQPADRLAELRQLVAQLDVPVRQVLIEARIVEANVDYEKSLGVKWGAPLQGEEARLGKDLFVDLGVE
RASSGVGLGLLRGGTLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSISFREASLSLEVTP
QITPDGKVIMAVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGQTIVIGGVYSTTQNKVVDKVPFLGDLPYVGRLFRR
DALQEKKSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1287 bp        

>NTDB_id=607621 K8374_RS22365 WP_224457244.1 4820897..4822183(-) (pilQ) [Pseudomonas sp. p1(2021b)]
ATGAGGTCATGGATGAGCGTGAACGGGCTGGGGAAATGGATGCTGGCGATACTCCTGACGGGCCAGGCCAGCATGGCGCT
GGCCAGTCGGAATGAGCCGCTCTCGCTGAACTTCCAGGACATAGAGGTGCGTGCGGCGCTGCAGGTGCTCGCCGACTTTG
CCGGTGTCAACCTGGTGGCCAGCGACACCGTGCAAGGCAACCTGACCCTGCGTCTGGAGGGGGTTCCCTGGGAGCAGGCC
CTTGACCTTGTGCTGCGCAGCAAGGGCTTGGCCCGACGTGAGGAGGGCAATGTCCTGCTGGTGGCACCGGCAGCCGAGTT
GGCCGAGCAATTTCGCGAAGCGCGCCTGGAGCAGGCGCTGGATGCGCAACAGCAGCCCATGCGCCGGGCATTGCTGCCGA
TTCACCACGCCAAGGCTGCGGACCTGGCCGAGCTGTTGCTGGCTGCACTGGCCGACGATGGCATCCTCACCGGGCGTGGC
AGCCTGAGCGTGGACGCCCGCACCAACACGTTGGTGGTCTACCAGCCTGCCGATCGGCTGGCTGAACTGCGACAGTTGGT
GGCGCAGTTGGACGTGCCGGTGCGCCAGGTGCTGATCGAGGCGCGTATCGTCGAGGCCAATGTCGACTATGAAAAGAGCC
TGGGGGTGAAATGGGGCGCGCCGCTGCAGGGCGAGGAGGCTCGCCTGGGCAAGGATTTGTTCGTCGATCTGGGCGTGGAG
CGGGCCAGTTCGGGTGTCGGGCTGGGCCTGCTACGTGGGGGCACGTTGCTGGACCTGGAACTCAGTGCCATGGAAAAGAG
CGGTAACGGCGAGATCATCTCCCAGCCGAAGGTGGTCACGGCGGACAAGGAAACGGCGCGGATCCTCAAGGGTACGGAGG
TCCCCTACCAGGAAACCAGCAAGAGCGGCGCCACCTCCATCTCGTTTCGCGAAGCATCGCTTTCATTGGAGGTCACGCCG
CAGATCACGCCGGACGGCAAGGTGATCATGGCGGTGAGGGTGACTAAGGACGAGCCTGACTACGTCAATGCACTGAACAA
CGTACCGCCGATCCGCAAGAACGAGGTCAACGCCAAGGTCCGGGTGGCCGATGGCCAGACCATCGTCATCGGTGGCGTCT
ACTCGACCACGCAAAACAAAGTGGTCGACAAGGTGCCATTTTTAGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGC
GATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAG
TCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)

Bacterial type II and III secretion system protein (Secretin)

(263-419)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.594

100

0.617
  comQ Acinetobacter baylyi ADP1

41.342

100

0.446
  pilQ Acinetobacter baumannii D1279779

40.618

100

0.43
  pilQ Acinetobacter baumannii strain A118

40.618

100

0.43
  pilQ Legionella pneumophila strain Lp02

44.31

96.495

0.428
  pilQ Legionella pneumophila strain ERS1305867

44.31

96.495

0.428
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.505

96.262

0.4
  pilQ Vibrio cholerae strain A1552

41.505

96.262

0.4
  pilQ Vibrio campbellii strain DS40M4

39.762

98.131

0.39