Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LA331_RS09310 Genome accession   NZ_CP083658
Coordinates   1874749..1876458 (+) Length   569 a.a.
NCBI ID   WP_005233533.1    Uniprot ID   N9NYN9
Organism   Acinetobacter variabilis strain FDAARGOS_1487     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1869749..1881458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA331_RS09265 (LA331_09265) nusA 1870326..1871810 (-) 1485 WP_004787201.1 transcription termination factor NusA -
  LA331_RS09270 (LA331_09270) rimP 1871846..1872370 (-) 525 WP_005233537.1 ribosome maturation factor RimP -
  LA331_RS09300 (LA331_09300) secG 1873335..1873664 (-) 330 WP_004787204.1 preprotein translocase subunit SecG -
  LA331_RS09305 (LA331_09305) tpiA 1873678..1874469 (-) 792 WP_005233535.1 triose-phosphate isomerase -
  LA331_RS09310 (LA331_09310) pilB 1874749..1876458 (+) 1710 WP_005233533.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LA331_RS09315 (LA331_09315) pilC 1876489..1877718 (+) 1230 WP_004787210.1 type II secretion system F family protein Machinery gene
  LA331_RS09320 (LA331_09320) pilD 1877718..1878578 (+) 861 WP_005233531.1 prepilin peptidase Machinery gene
  LA331_RS09325 (LA331_09325) coaE 1878580..1879185 (+) 606 WP_005233529.1 dephospho-CoA kinase -
  LA331_RS09330 (LA331_09330) - 1879224..1880141 (-) 918 WP_005233528.1 DMT family transporter -
  LA331_RS09335 (LA331_09335) rlmB 1880187..1880936 (-) 750 WP_005233526.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  LA331_RS09340 (LA331_09340) - 1881079..1881402 (-) 324 WP_004787221.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63437.44 Da        Isoelectric Point: 4.7923

>NTDB_id=607186 LA331_RS09310 WP_005233533.1 1874749..1876458(+) (pilB) [Acinetobacter variabilis strain FDAARGOS_1487]
MTALQGSPRFTGFIRRLVDEGVLSAEDMRTALANAKQEKTDIVAYLVSHHNLSPTTIAETISVEFGEPLFDIGAYDPGQI
LREDIDEKLITKHRILPIYKNSNVLYVATSNPTNIEATDAIRFATKLNIETVIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVNVDTADPTQEEDEKDKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGFT
ESDLQSPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLVKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=607186 LA331_RS09310 WP_005233533.1 1874749..1876458(+) (pilB) [Acinetobacter variabilis strain FDAARGOS_1487]
ATGACAGCATTGCAGGGGTCACCAAGATTTACGGGGTTTATTCGTCGCCTGGTCGATGAAGGGGTATTATCGGCTGAAGA
TATGCGTACTGCTTTAGCCAATGCCAAACAAGAAAAAACGGATATCGTGGCCTATTTGGTCAGTCACCATAATCTTTCCC
CTACCACCATTGCTGAAACGATCTCAGTAGAATTTGGTGAGCCTTTATTTGATATTGGCGCTTATGATCCAGGACAAATC
CTGCGTGAAGATATCGATGAAAAGCTAATTACTAAACATCGAATTTTGCCAATTTATAAGAATTCCAATGTTCTTTATGT
TGCCACGAGCAATCCAACCAATATTGAAGCAACGGATGCAATTCGTTTTGCCACCAAACTGAATATTGAAACCGTTATTG
TTGAACACAATAAACTCGAGAAACTGATCGAGCAAAACTTTACTGAAGAAAGTACTTTTGATTTCGACGATGATTTCGAT
CTGGATGTAAATGTCGATACAGCCGATCCGACTCAAGAAGAAGACGAAAAAGACAAAGGTGAAGAAGCCCCGATTGTCAA
ATATATTAATAAATTACTGATTGATGCCATTCGTATGGGCGCATCGGATCTACATTTTGAACCTTATGAAAAAATGTATC
GGGTACGCTATCGTGTTGATGGAGTCTTACGTCAAATTGCAACACCGCCGCTACAACTAGCAAACCGTTTGGCGTCACGT
TTAAAAGTTATGTCGCAAATGGACATTTCTGAGAAACGCGTGCCTCAAGACGGCCGTATTAAATTAAAGCTTTCAAAAAA
CAAAGCTATCGACTTCCGTGTTAACTCACTCCCTACCCTGTTTGGTGAAAAACTGGTTCTACGTATTCTAGATCCGTCTA
GTGCCATGCTGGGAATTGATGCTTTAGGTTATGAACCTGAGCAGAAAGAGTTATTCATGGAAGCCTTAGATAAACCGCAA
GGGATGTTACTGATTACCGGTCCTACTGGTTCCGGTAAAACCGTATCCCTTTATACGGGCTTAAACATCTTAAATCGTGA
AGATACCAATATTTCTACTGCGGAAGACCCAGTCGAGATTAATCTGCAAGGGATTAATCAGGTGAACGTCAACAATAAAG
TTGGCCTGACTTTCGCAGCGGCATTAAAATCCTTCTTGCGTCAGGATCCAGATATCGTCATGGTTGGTGAGATCCGAGAT
CTAGAAACAGCTGAAATTGCAATTAAAGCAGCCCAAACAGGTCATATGGTGATGTCAACCCTGCACACCAACAGTGCTCC
GGAAACCCTGACCCGTTTACGTAATATGGGCGTCCCTTCTTTCAACATTGCGACCTCGGTCAATCTAGTGATTGCGCAGC
GTCTGGCCCGTCGCTTGTGTTCACAATGTAAAAAGCCGGCTGATATTCCTCAGCAAAGTTTGCTGGAAATGGGCTTTACC
GAATCAGATTTACAAAGCCCTGAATTTCAGATTTATGAGCCAGTCGGCTGTAATGAATGCCGTGAAGGGTATAAGGGCCG
GGTCGGTATTTACGAAGTCATGAAAGTCACACCTGAAATTTCAAGAATTATTATGGAAGATGGCAATGCCATTGAAATTG
CTGAAGCTTCCGCGCGTGCAGGATTTAACAATTTACGCCGATCAGGGTTAGTGAAGGTTATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTAACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9NYN9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.053

100

0.812
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

51.413

99.473

0.511
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

49.013

97.891

0.48
  pilB Vibrio campbellii strain DS40M4

48.825

97.188

0.475
  pilB Vibrio cholerae strain A1552

50.192

91.388

0.459
  pilF Thermus thermophilus HB27

38.313

100

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

34.258

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.319

97.012

0.362