Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NMD49_RS19765 Genome accession   NZ_CP100927
Coordinates   3972765..3974150 (+) Length   461 a.a.
NCBI ID   WP_001025199.1    Uniprot ID   -
Organism   Escherichia coli strain ET729     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3967765..3979150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD49_RS19740 (NMD49_19580) aroP 3968313..3969686 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  NMD49_RS19745 (NMD49_19585) ampE 3969729..3970583 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  NMD49_RS19750 (NMD49_19590) ampD 3970580..3971131 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD49_RS19755 (NMD49_19595) nadC 3971219..3972112 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD49_RS19760 (NMD49_19600) pilA 3972315..3972755 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  NMD49_RS19765 (NMD49_19605) pilB 3972765..3974150 (+) 1386 WP_001025199.1 type II secretion system protein GspE Machinery gene
  NMD49_RS19770 (NMD49_19610) hofC 3974140..3975342 (+) 1203 WP_000157262.1 protein transport protein HofC -
  NMD49_RS19775 (NMD49_19615) guaC 3975377..3976420 (-) 1044 WP_001217338.1 GMP reductase -
  NMD49_RS19780 (NMD49_19620) - 3976576..3976620 (-) 45 WP_120795372.1 protein YacM -
  NMD49_RS19785 (NMD49_19625) coaE 3976645..3977265 (+) 621 WP_001269527.1 dephospho-CoA kinase -
  NMD49_RS19790 (NMD49_19630) zapD 3977265..3978008 (+) 744 WP_001194722.1 cell division protein ZapD -
  NMD49_RS19795 (NMD49_19635) yacG 3978018..3978215 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  NMD49_RS19800 (NMD49_19640) mutT 3978431..3978820 (-) 390 WP_000736009.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50610.29 Da        Isoelectric Point: 6.5405

>NTDB_id=607067 NMD49_RS19765 WP_001025199.1 3972765..3974150(+) (pilB) [Escherichia coli strain ET729]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGELIHIPDTVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=607067 NMD49_RS19765 WP_001025199.1 3972765..3974150(+) (pilB) [Escherichia coli strain ET729]
ATGAATATTCCACAGCTCACGGCCCTGTGCCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAGTTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTTAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACCGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGGCTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGTGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCTCATCCATATTCCAGACACTGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGTACGGCCTTATTTGAA
GTTCTGCCCATAACGCCGGTCATTCGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCACGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTAGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TACTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)

Type II/IV secretion system protein (T2SSE)

(88-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50.773

84.165

0.427
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Acinetobacter baylyi ADP1

39.655

100

0.399
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.753

83.514

0.39
  pilB Haemophilus influenzae 86-028NP

45.478

83.948

0.382
  pilB Haemophilus influenzae Rd KW20

44.703

83.948

0.375
  pilB Acinetobacter baumannii D1279779

43.846

84.599

0.371
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilF Thermus thermophilus HB27

41.542

87.202

0.362