Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NMD49_RS19760 Genome accession   NZ_CP100927
Coordinates   3972315..3972755 (+) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain ET729     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3967315..3977755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD49_RS19740 (NMD49_19580) aroP 3968313..3969686 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  NMD49_RS19745 (NMD49_19585) ampE 3969729..3970583 (-) 855 WP_000172014.1 beta-lactamase regulator AmpE -
  NMD49_RS19750 (NMD49_19590) ampD 3970580..3971131 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NMD49_RS19755 (NMD49_19595) nadC 3971219..3972112 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NMD49_RS19760 (NMD49_19600) pilA 3972315..3972755 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  NMD49_RS19765 (NMD49_19605) pilB 3972765..3974150 (+) 1386 WP_001025199.1 type II secretion system protein GspE Machinery gene
  NMD49_RS19770 (NMD49_19610) hofC 3974140..3975342 (+) 1203 WP_000157262.1 protein transport protein HofC -
  NMD49_RS19775 (NMD49_19615) guaC 3975377..3976420 (-) 1044 WP_001217338.1 GMP reductase -
  NMD49_RS19780 (NMD49_19620) - 3976576..3976620 (-) 45 WP_120795372.1 protein YacM -
  NMD49_RS19785 (NMD49_19625) coaE 3976645..3977265 (+) 621 WP_001269527.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=607066 NMD49_RS19760 WP_000360901.1 3972315..3972755(+) (pilA) [Escherichia coli strain ET729]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=607066 NMD49_RS19760 WP_000360901.1 3972315..3972755(+) (pilA) [Escherichia coli strain ET729]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGTGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363