Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LA358_RS00220 Genome accession   NZ_CP083612
Coordinates   51851..53557 (+) Length   568 a.a.
NCBI ID   WP_042025094.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain FDAARGOS_1536     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 46851..58557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA358_RS00195 (LA358_00195) pdhR 48079..48843 (-) 765 WP_224210522.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  LA358_RS00200 (LA358_00200) ampD 49110..49691 (-) 582 WP_224210523.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LA358_RS00205 (LA358_00205) - 49715..50185 (+) 471 WP_224210524.1 TIGR02281 family clan AA aspartic protease -
  LA358_RS00210 (LA358_00210) nadC 50189..51046 (+) 858 WP_224210525.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LA358_RS20080 - 51420..51851 (+) 432 WP_328706056.1 pilin -
  LA358_RS00220 (LA358_00220) pilB 51851..53557 (+) 1707 WP_042025094.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  LA358_RS00225 (LA358_00225) pilC 53779..55020 (+) 1242 WP_026456598.1 type II secretion system F family protein Machinery gene
  LA358_RS00230 (LA358_00230) pilD 55087..55962 (+) 876 WP_224210526.1 prepilin peptidase Machinery gene
  LA358_RS00235 (LA358_00235) coaE 55966..56580 (+) 615 WP_224210527.1 dephospho-CoA kinase -
  LA358_RS00240 (LA358_00240) zapD 56618..57340 (+) 723 WP_224210528.1 cell division protein ZapD -
  LA358_RS00245 (LA358_00245) yacG 57350..57544 (+) 195 WP_026457308.1 DNA gyrase inhibitor YacG -
  LA358_RS00250 (LA358_00250) mutT 57607..58002 (-) 396 WP_224211544.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62535.94 Da        Isoelectric Point: 5.5619

>NTDB_id=606617 LA358_RS00220 WP_042025094.1 51851..53557(+) (pilB) [Aeromonas enteropelogenes strain FDAARGOS_1536]
MTSSPHSGLAISLAASSLISEEDSQRYLSQAKAQRKPFVAFLVENQILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLLENETDVLGMEDIDESEI
SDLEVSDEGSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=606617 LA358_RS00220 WP_042025094.1 51851..53557(+) (pilB) [Aeromonas enteropelogenes strain FDAARGOS_1536]
ATGACCTCTAGCCCCCATAGCGGCCTGGCGATAAGCCTGGCCGCCTCTTCGTTGATCAGCGAAGAGGATTCCCAGCGCTA
CCTGAGTCAGGCCAAGGCCCAGCGCAAACCCTTCGTGGCATTCCTGGTGGAGAACCAGATCCTCGACAGCAAGGCGTTGG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACATCATGTGCTGCCCATCTACACCCAAGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAAGATTTCGGCTTCAGCTTCGGGCTCCATACCGAGGCGCTGCTGGTGGAAGAAAACA
AGCTCACGACCGCCATCGGCAAGCTGCTGGAAAACGAGACCGACGTGCTCGGCATGGAGGATATTGACGAGTCGGAGATC
TCGGATCTCGAGGTTTCCGACGAGGGCAGTCGCCTCGACGAGAGTGTCAACACCACGGACGATGACGCCCCCATCGTCAA
GTACATTAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGATCTGCACTTCGAGCCCTATGAGCAGAAGTACC
GCATCCGCTTTCGCATCGATGGCATCCTGCACGAAATCGCCACACCACCAGTCAATCTGGCCAACCGCTTCTCGGCCCGC
CTCAAGGTGATGGCGCGGCTCGATATCGCCGAGCGGCGCCTGCCCCAGGATGGGCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTGATCCGTCTGCTGGACTCCTCGG
CGGCGCGCCTCAATATCGAGCAGCTCGGCTTTGACGATCGGCAGAAAGCGCAATACCTGCGCGCCCTCTCCAAACCCCAG
GGGATGATCCTGGTGACCGGTCCCACCGGCTCGGGCAAAACGGTCTCGCTTTATACCGGCCTCAACATTCTCAACACCAC
AGAGGTGAATATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGGTCAATCCCAAAG
CAGGCCTCACCTTCGCCAGCGCACTGCGCTCCTTCCTGCGTCAGGATCCTGATGTTGTGATGGTCGGGGAAATACGGGAC
TTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACTAACTCCGCTGC
CGAAACTCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCTCGCAAGCTGTGCGATCACTGCAAAGCGCCCGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTTACC
CCGCAGCAACTGGCTGCGGGGCTGAGGTTGTTCAAGCCGGTGGGTTGCAAGGAGTGCTCGGGCGGCTACAAGGGACGGGT
CGGCATCTACGAAATCATGCTGATGTCGGAAAATATCGCAAAATTGATCATGCAGGGAGCCAACTCGCTGCAGATCGCCG
CCATCGCCCAGAAGGAAGGGATGCGAACCCTGCGCACCTCCGGGTTGGAAAAGGCCCGCCTAGGTGTCACCAGTCTGGCG
GAAGTGAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.415

100

0.565
  pilB Acinetobacter baylyi ADP1

55.81

100

0.558
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

53.996

99.12

0.535
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilB Vibrio campbellii strain DS40M4

50.348

100

0.509
  pilF Neisseria gonorrhoeae MS11

46.986

99.296

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

38.062

100

0.387
  pilF Thermus thermophilus HB27

40.341

92.958

0.375