Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K9838_RS16295 Genome accession   NZ_CP083575
Coordinates   3692960..3694669 (-) Length   569 a.a.
NCBI ID   WP_017155147.1    Uniprot ID   -
Organism   Xanthomonas phaseoli pv. manihotis strain CHN01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3687960..3699669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9838_RS16285 (K9838_16295) - 3689170..3690783 (+) 1614 WP_017155150.1 HAMP domain-containing sensor histidine kinase -
  K9838_RS16290 (K9838_16300) pilR 3691055..3692509 (+) 1455 WP_017155148.1 sigma-54 dependent transcriptional regulator Regulator
  K9838_RS16295 (K9838_16305) pilB 3692960..3694669 (-) 1710 WP_017155147.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K9838_RS16300 (K9838_16310) - 3694724..3695134 (-) 411 WP_033474088.1 pilin -
  K9838_RS16305 (K9838_16315) pilA/pilAI 3695238..3695666 (-) 429 WP_017155146.1 pilin Machinery gene
  K9838_RS16310 (K9838_16320) pilC 3695997..3697253 (+) 1257 WP_017155145.1 type II secretion system F family protein Machinery gene
  K9838_RS16315 (K9838_16325) - 3697260..3698123 (+) 864 WP_017155144.1 A24 family peptidase -
  K9838_RS16320 (K9838_16330) coaE 3698137..3698748 (+) 612 WP_017155143.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61658.61 Da        Isoelectric Point: 5.4503

>NTDB_id=606260 K9838_RS16295 WP_017155147.1 3692960..3694669(-) (pilB) [Xanthomonas phaseoli pv. manihotis strain CHN01]
MGITGIARRLVQDGALDESVARSAMDQAAAAKVPLPQWFAEKKLVSAAQLAAANAIEFGMPLMDVSAFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLGNDDEEMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDNALLAEGF
TPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKAANGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=606260 K9838_RS16295 WP_017155147.1 3692960..3694669(-) (pilB) [Xanthomonas phaseoli pv. manihotis strain CHN01]
ATGGGCATTACTGGCATCGCCCGCCGCCTGGTACAGGATGGCGCACTCGACGAATCCGTCGCCCGCAGTGCCATGGACCA
GGCTGCCGCGGCCAAGGTGCCATTGCCGCAATGGTTCGCCGAAAAAAAACTAGTATCGGCGGCGCAGTTGGCAGCAGCCA
ATGCCATTGAATTCGGCATGCCTCTAATGGACGTATCGGCGTTTGACGCCAGCCAGAACGCGGTGAAGCTGGTCAGCGAG
GAGTTGCTCCAGAAGCATCAGGTGCTGCCGCTGTTCAAGCGCGGTAACCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCATTCTTGTCGACGAGGATCAGATCCGCC
GGACGCTCGAACAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCGTCGCTTGGCAACGACGACGAGGAAATGGGGGATCTC
GACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGACGACT
ACCGGGTGCGCTTGCGCATCGACGGCCTGCTGAAGAATGTGGCCAAGGCGCCGGTGAAGCTGAATCAGCGCATCGCAGCA
CGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGTGAGAAGGTGGTGCTGCGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGTATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAGTTGTTCCTGGAGGCGATCCACAAGCCC
TATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTTGTATACCGCGCTGGGAATTCTCAACGA
CGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTGGCGTCAATCAGGTACAGCAGAACAACA
AGCGTGGCATGACCTTCGCCGCAGCCTTGCGCTCGTTCCTGCGACAGGATCCGGACATCATCATGGTTGGCGAAATCCGC
GACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCGACGTTGCACACCAACGATGC
GCCACAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCCTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGGCTGGCGCGGCGGTTGTGTAACAACTGCAAGCGCAAGTCGACGCTGCCTGATAATGCATTGCTGGCCGAAGGATTC
ACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTTGGCTGCGACGAATGCACCGAAGGCTACAAGGGGCG
TACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGCTGGAAGGCGGCAATGCAATGCAGATCG
CCGAGGCCGCGCAGGCGATCGGGATCCGCGATTTGCGTCAATCGGCGCTGATGAAGGCTGCGAATGGGGTGACCAGCCTG
GCGGAGATCAATCGAGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.338

99.824

0.562
  pilB Acinetobacter baylyi ADP1

55.282

99.824

0.552
  pilB Legionella pneumophila strain ERS1305867

52.724

100

0.527
  pilF Neisseria gonorrhoeae MS11

49.472

99.824

0.494
  pilB Vibrio cholerae strain A1552

50

97.364

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

98.418

0.462
  pilB Vibrio campbellii strain DS40M4

45.87

100

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.13

92.97

0.364
  pilF Thermus thermophilus HB27

39.423

91.388

0.36