Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K9838_RS14865 Genome accession   NZ_CP083575
Coordinates   3336595..3337725 (-) Length   376 a.a.
NCBI ID   WP_008571827.1    Uniprot ID   A0A3Q9Q1S7
Organism   Xanthomonas phaseoli pv. manihotis strain CHN01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3331595..3342725
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K9838_RS14845 (K9838_14855) - 3331890..3333722 (+) 1833 WP_017163753.1 DUF4153 domain-containing protein -
  K9838_RS14850 (K9838_14860) - 3333733..3334305 (+) 573 WP_017157669.1 DNA-3-methyladenine glycosylase I -
  K9838_RS14855 (K9838_14865) - 3334313..3335247 (+) 935 Protein_2904 DUF72 domain-containing protein -
  K9838_RS14860 (K9838_14870) - 3335415..3336089 (-) 675 WP_016851096.1 YitT family protein -
  K9838_RS14865 (K9838_14875) pilU 3336595..3337725 (-) 1131 WP_008571827.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K9838_RS14870 (K9838_14880) pilT 3337838..3338875 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  K9838_RS14875 (K9838_14885) - 3339294..3339986 (+) 693 WP_017157671.1 YggS family pyridoxal phosphate-dependent enzyme -
  K9838_RS14880 (K9838_14890) proC 3340030..3340884 (+) 855 WP_017157672.1 pyrroline-5-carboxylate reductase -
  K9838_RS14885 (K9838_14895) - 3341441..3341866 (+) 426 WP_017159922.1 HU family DNA-binding protein -
  K9838_RS14890 (K9838_14900) - 3341969..3342610 (+) 642 WP_017157674.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42014.24 Da        Isoelectric Point: 6.8286

>NTDB_id=606255 K9838_RS14865 WP_008571827.1 3336595..3337725(-) (pilU) [Xanthomonas phaseoli pv. manihotis strain CHN01]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=606255 K9838_RS14865 WP_008571827.1 3336595..3337725(-) (pilU) [Xanthomonas phaseoli pv. manihotis strain CHN01]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGATCTGTTCATCACCTCGGGGAT
GCCGCCGGCGATCAAGGTGCACGGTAAGATCAGCCCGATCACCCAGACGCCGTTGACCGCGCAGCAGAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTTCGTGTCAGCTGTTTCTACCAGCGTAACCAGGTGGGCATGGTGTTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCTTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGGACCGGTAAGTCGACCTCGCTGGCGGCGATGATTGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATTACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGATCGGCGCAACCAGCTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGCCGCGGCCGCCGCGTGGCGATGGAAATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGCTACGAAGACGCGTTGCGCTACGCCGACTCGCAGAACGA
AGTGCGTCTGCGCATCAAACTCTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGAGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9Q1S7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.03

97.606

0.654
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367