Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LAG74_RS01940 Genome accession   NZ_CP083461
Coordinates   397795..399510 (-) Length   571 a.a.
NCBI ID   WP_224969342.1    Uniprot ID   -
Organism   Aeromonas veronii strain SW3814     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 392795..404510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAG74_RS01910 (LAG74_01915) mutT 393305..393709 (+) 405 WP_224969339.1 8-oxo-dGTP diphosphatase MutT -
  LAG74_RS01915 (LAG74_01920) yacG 393787..393981 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  LAG74_RS01920 (LAG74_01925) zapD 393991..394713 (-) 723 WP_005336141.1 cell division protein ZapD -
  LAG74_RS01925 (LAG74_01930) coaE 394751..395365 (-) 615 WP_224969340.1 dephospho-CoA kinase -
  LAG74_RS01930 (LAG74_01935) pilD 395379..396251 (-) 873 WP_224969341.1 A24 family peptidase Machinery gene
  LAG74_RS01935 (LAG74_01940) pilC 396313..397554 (-) 1242 WP_111899845.1 type II secretion system F family protein Machinery gene
  LAG74_RS01940 (LAG74_01945) pilB 397795..399510 (-) 1716 WP_224969342.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  LAG74_RS01945 (LAG74_01950) - 399501..399908 (-) 408 WP_224970155.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LAG74_RS01950 (LAG74_01955) nadC 400235..401110 (-) 876 WP_224969343.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LAG74_RS01955 (LAG74_01960) - 401114..401584 (-) 471 WP_224969344.1 retropepsin-like aspartic protease -
  LAG74_RS01960 (LAG74_01965) ampD 401716..402291 (+) 576 WP_224969345.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LAG74_RS01965 (LAG74_01970) pdhR 402570..403334 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62915.37 Da        Isoelectric Point: 5.4298

>NTDB_id=606007 LAG74_RS01940 WP_224969342.1 397795..399510(-) (pilB) [Aeromonas veronii strain SW3814]
MPIMTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKHFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDE
SEISDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTPQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEVNRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=606007 LAG74_RS01940 WP_224969342.1 397795..399510(-) (pilB) [Aeromonas veronii strain SW3814]
TTGCCCATAATGACATCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTC
CCAGCGCTATCTGAGCCAGGCCAAGGCCCAGCGCAAGCACTTTGTCACCTTTCTGATCGAGAACGAGATCCTCGATAGCA
AGGCGCTGGCCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAGCACCACGTCCTGCCCATCTACACTCAGGGCCACACCCTCTATATCGC
CATGTCGGATCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAGGCGCTGCTGGTGG
AGGAGAACAAGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGATGCCCTCGGCATGGAGGATATCGACGAA
TCCGAGATTTCGGATCTCGAGGTCTCCGACGAGAACAGTCGGCTGGACGAAAGCGTCAATACAGCTGATGACGATGCTCC
CATCGTCAAGTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCATTTCGAGCCTTACGAGT
TCAAGTATCGGGTTCGCTTTCGCATCGACGGCATTCTGCATGAAATCGCCACACCGCCGGTCAACCTCTCCAACCGCTTC
TCAGCTCGCCTCAAGGTGATGGCGCGACTCGATATCGCCGAACGGCGGCTGCCGCAGGATGGCCGCATCAAGCTGAAGCT
GTCGCGCAACAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCG
ACTCCTCGGCGGCACGCCTCAATATCGAACAACTCGGCTTTGATGACCGGCAGAAAGCGCAGTACCTGCGTGCCCTCTCC
AAACCACAAGGAATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCT
CAACACCACCGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCA
ATCCCAAGGCGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGTGAA
ATACGGGATCTAGAAACCGCCGAAATCGCCATCAAGGCGGCACAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAA
CTCGGCTGCCGAAACCCTGACCCGAATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCA
TGGCCCAGCGGCTGGCCCGTAAACTGTGCGACCACTGCAAGGCACCGGAAGTGGTGCCGGAAGCAGAACTGCTGGAGCTG
GGCTTTACGCCGCAGCAGCTGGCAGCCGGGCTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAA
GGGGCGAGTCGGCATTTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAAGGGGCCAACTCCCTGC
AAATCGCCGCGATCGCCCAAAAAGAGGGGATGCGTACCCTGCGCACCTCGGGGCTGGAGAAGGCCCGGCTCGGGGTCACC
AGTCTGGCCGAGGTCAACCGGGTCACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.672

100

0.559
  pilB Acinetobacter baylyi ADP1

55.672

100

0.559
  pilB Legionella pneumophila strain ERS1305867

56.642

94.921

0.538
  pilB Vibrio cholerae strain A1552

54.758

97.548

0.534
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

98.774

0.51
  pilF Neisseria gonorrhoeae MS11

46.809

98.774

0.462
  pilF Thermus thermophilus HB27

39.421

96.848

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

96.848

0.378