Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K8O95_RS00205 Genome accession   NZ_CP082887
Coordinates   51638..53353 (+) Length   571 a.a.
NCBI ID   WP_223379347.1    Uniprot ID   -
Organism   Aeromonas enteropelogenes strain FDAARGOS_1459     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 46638..58353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8O95_RS00180 (K8O95_00180) pdhR 47865..48629 (-) 765 WP_026456603.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  K8O95_RS00185 (K8O95_00185) ampD 48896..49477 (-) 582 WP_124238861.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K8O95_RS00190 (K8O95_00190) - 49501..49971 (+) 471 WP_223379341.1 TIGR02281 family clan AA aspartic protease -
  K8O95_RS00195 (K8O95_00195) nadC 49975..50832 (+) 858 WP_223379343.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K8O95_RS00200 (K8O95_00200) - 51207..51647 (+) 441 WP_223379345.1 pilin -
  K8O95_RS00205 (K8O95_00205) pilB 51638..53353 (+) 1716 WP_223379347.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  K8O95_RS00210 (K8O95_00210) pilC 53573..54814 (+) 1242 WP_026456598.1 type II secretion system F family protein Machinery gene
  K8O95_RS00215 (K8O95_00215) pilD 54881..55756 (+) 876 WP_223379349.1 prepilin peptidase Machinery gene
  K8O95_RS00220 (K8O95_00220) coaE 55760..56374 (+) 615 WP_223379351.1 dephospho-CoA kinase -
  K8O95_RS00225 (K8O95_00225) zapD 56412..57134 (+) 723 WP_201980463.1 cell division protein ZapD -
  K8O95_RS00230 (K8O95_00230) yacG 57144..57338 (+) 195 WP_026457308.1 DNA gyrase inhibitor YacG -
  K8O95_RS00235 (K8O95_00235) mutT 57401..57796 (-) 396 WP_026457309.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62894.41 Da        Isoelectric Point: 5.5637

>NTDB_id=603354 K8O95_RS00205 WP_223379347.1 51638..53353(+) (pilB) [Aeromonas enteropelogenes strain FDAARGOS_1459]
MPIMTSSPHSGLAISLAASSLISEEDSQRYLSQAKAQRKPFVAFLVENQILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMENENDALGMEEIDE
SEISDLEVSDEGSRLDESVNTTDDDAPIVKYINKILMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTPQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEVNRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=603354 K8O95_RS00205 WP_223379347.1 51638..53353(+) (pilB) [Aeromonas enteropelogenes strain FDAARGOS_1459]
TTGCCCATAATGACCTCTAGCCCCCATAGCGGCCTGGCGATAAGCCTGGCCGCCTCTTCGTTGATCAGCGAAGAGGATTC
CCAGCGCTACCTGAGTCAGGCCAAAGCCCAGCGCAAACCCTTCGTGGCATTTCTGGTGGAGAACCAGATCCTCGACAGCA
AGGCGTTGGCAGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATCTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCGGATCCGACCAATGTGTCGGCACTGGAAGATTTCGGCTTCAGCTTCGGGCTCCATACCGAGGCGCTGCTGGTGG
AAGAAAACAAGCTCACCACCGCCATCGGCAAGCTGATGGAGAACGAAAACGACGCCCTCGGCATGGAGGAGATCGACGAG
TCCGAAATCTCGGATCTCGAAGTCTCCGACGAGGGCAGCCGCCTCGACGAGAGCGTCAATACCACGGACGATGACGCCCC
CATCGTCAAGTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAACCCTATGAGC
AGAAGTACCGCATCCGCTTTCGCATTGACGGCATCCTGCACGAAATCGCCACGCCACCGGTCAATCTGGCCAACCGCTTC
TCGGCCCGCCTCAAGGTGATGGCACGGCTCGATATCGCCGAACGGCGCCTGCCCCAGGATGGCCGCATCAAGCTGAAGCT
GTCACGCAACAAGTCGATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTGG
ACTCCTCGGCGGCGCGCCTCAATATCGAGCAGCTCGGCTTTGACGATCGGCAGAAAGCGCAATACCTGCGCGCCCTCTCC
AAACCCCAGGGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACAGTCTCGCTTTACACCGGCCTCAATATTCT
CAACACCACAGAGGTGAATATCTCCACCGCCGAGGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCA
ATCCCAAGGCGGGACTTACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGTCAGGATCCTGATGTGGTGATGGTCGGGGAA
ATACGGGACTTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAAACCGGCCACCTGGTGCTCTCCACCCTGCACACCAA
CTCAGCGGCCGAAACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCA
TGGCCCAGCGGCTGGCTCGCAAGCTGTGCGATCACTGCAAGGCTCCCGAAGTGGTGCCGGAGGCCGAATTGCTGGAGCTG
GGCTTTACCCCGCAGCAACTGGCAGCGGGGCTGCGGCTGTTCAAACCGGTAGGTTGCAAGGAGTGCTCTGGCGGCTACAA
GGGACGGGTCGGCATCTACGAAATCATGCTGATGTCGGAAAATATCGCAAAATTGATCATGCAGGGGGCCAACTCGCTGC
AGATCGCCGCCATCGCCCAGAAAGAGGGGATGCGGACCCTGCGCACCTCCGGGTTGGAGAAGGCACGCCTGGGTGTTACC
AGTCTGGCGGAAGTGAACCGGGTCACCACCAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.042

100

0.56
  pilB Acinetobacter baylyi ADP1

55.672

100

0.559
  pilB Legionella pneumophila strain ERS1305867

56.538

95.096

0.538
  pilB Vibrio cholerae strain A1552

54.352

98.599

0.536
  pilB Vibrio campbellii strain DS40M4

51.59

99.124

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

51.601

98.424

0.508
  pilF Neisseria gonorrhoeae MS11

46.986

98.774

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

38.07

99.825

0.38
  pilF Thermus thermophilus HB27

40.832

92.644

0.378