Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6750_RS02845 Genome accession   NZ_CP082313
Coordinates   584921..586606 (-) Length   561 a.a.
NCBI ID   WP_132936924.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain ZZV2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 579921..591606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6750_RS02815 (K6750_02815) rplS 579996..580349 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  K6750_RS02820 (K6750_02820) yacG 581004..581198 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  K6750_RS02825 (K6750_02825) zapD 581357..582097 (-) 741 WP_042521326.1 cell division protein ZapD -
  K6750_RS02830 (K6750_02830) coaE 582125..582739 (-) 615 WP_017635920.1 dephospho-CoA kinase -
  K6750_RS02835 (K6750_02835) pilD 582740..583609 (-) 870 WP_017635919.1 A24 family peptidase Machinery gene
  K6750_RS02840 (K6750_02840) pilC 583674..584897 (-) 1224 WP_238966838.1 type II secretion system F family protein Machinery gene
  K6750_RS02845 (K6750_02845) pilB 584921..586606 (-) 1686 WP_132936924.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6750_RS02850 (K6750_02850) - 586609..587094 (-) 486 WP_025768185.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K6750_RS02855 (K6750_02855) nadC 587357..588244 (-) 888 WP_047102656.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K6750_RS02860 (K6750_02860) ampD 588337..588888 (+) 552 WP_213899256.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K6750_RS02865 (K6750_02865) pdhR 589294..590061 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62061.95 Da        Isoelectric Point: 5.8428

>NTDB_id=601282 K6750_RS02845 WP_132936924.1 584921..586606(-) (pilB) [Vibrio alginolyticus strain ZZV2]
MLTNLSTVLRQAGILSFSQEESLLEQAKASGISMPEALLSCGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLTLAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRALGQEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSKQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=601282 K6750_RS02845 WP_132936924.1 584921..586606(-) (pilB) [Vibrio alginolyticus strain ZZV2]
ATGCTAACCAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGTCTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
TAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTGCTCAGTTGTGGCTTTTTCCAATCACATGAGCTGACTGAGCACCTTA
GCTCTATCTTTGGACTTTCCTGCACATCACTCAGTCAGTATGAGTACTCGTCGCTGTGCCAAAAGCTTGGCTTGCGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCCACATTAACACTCGCGGTTGCAGACCCAACTAACTT
ACAAGCGGAAGATGACTTTCGTTTTGCGACAGGGTTGCAAGTAGAGCTTGTTCTCGCTGATTTTCGCGAGCTGACCGCTG
CGATTCGGCGCTTATATGGCCGAGCACTTGGGCAAGAAAAGTCTGGATTAAAAGAGATCAACCAAGAAGAATTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGACGAATCTCCTGTTAGTCGTTATATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTTCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCCGAAAGGCGCTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCTACTCTATTTGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCAAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGGCCAACCGGGAGCGGAAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCCGATGTAGTAATGGTCGGTGAGATTCGTGATTTAGATACC
GCCGAAATCGCCATAAAAGCATCTCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

92.87

100

0.929
  pilB Vibrio parahaemolyticus RIMD 2210633

92.692

100

0.927
  pilB Vibrio cholerae strain A1552

74.377

100

0.745
  pilB Acinetobacter baumannii D1279779

51.731

92.692

0.48
  pilB Acinetobacter baylyi ADP1

47.67

99.465

0.474
  pilB Legionella pneumophila strain ERS1305867

48.803

96.791

0.472
  pilF Neisseria gonorrhoeae MS11

43.972

100

0.442
  pilB/pilB1 Synechocystis sp. PCC 6803

36.634

100

0.396
  pilF Thermus thermophilus HB27

36.299

100

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.315

99.643

0.362