Detailed information NTG0601280

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: K6750_RS02835
• Genome accession: NZ_CP082313
• Coordinates: 582740..583609 (-)
• Length: 289 a.a.
• NCBI ID: WP_017635919.1
• Uniprot ID: A0A2I3C9C8
• Organism: Vibrio alginolyticus strain ZZV2
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 577740..588609

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6750_RS02800 (K6750_02800)	rpsP	578339..578587 (+)	249	WP_005379962.1	30S ribosomal protein S16	-
K6750_RS02805 (K6750_02805)	rimM	578617..579165 (+)	549	WP_005379964.1	ribosome maturation factor RimM	-
K6750_RS02810 (K6750_02810)	trmD	579193..579954 (+)	762	WP_005385420.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
K6750_RS02815 (K6750_02815)	rplS	579996..580349 (+)	354	WP_005379971.1	50S ribosomal protein L19	-
K6750_RS02820 (K6750_02820)	yacG	581004..581198 (-)	195	WP_017635922.1	DNA gyrase inhibitor YacG	-
K6750_RS02825 (K6750_02825)	zapD	581357..582097 (-)	741	WP_042521326.1	cell division protein ZapD	-
K6750_RS02830 (K6750_02830)	coaE	582125..582739 (-)	615	WP_017635920.1	dephospho-CoA kinase	-
K6750_RS02835 (K6750_02835)	pilD	582740..583609 (-)	870	WP_017635919.1	A24 family peptidase	Machinery gene
K6750_RS02840 (K6750_02840)	pilC	583674..584897 (-)	1224	WP_238966838.1	type II secretion system F family protein	Machinery gene
K6750_RS02845 (K6750_02845)	pilB	584921..586606 (-)	1686	WP_132936924.1	type IV-A pilus assembly ATPase PilB	Machinery gene
K6750_RS02850 (K6750_02850)	-	586609..587094 (-)	486	WP_025768185.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
K6750_RS02855 (K6750_02855)	nadC	587357..588244 (-)	888	WP_047102656.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32563.76 Da
• Isoelectric Point: 6.8450

>NTDB_id=601280 K6750_RS02835 WP_017635919.1 582740..583609(-) (pilD) [Vibrio alginolyticus strain ZZV2]
MEVFQYYPWLFVVFATIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIEPPKEVLTLSVPRSSCQKCATPIRIR
DNIPVISWLLLKGKCHNCQATISARYPLIELLTAFCSGFIAYYFGFSYFTVALLFFTFILIAATFIDLDTMLLPDQLTLP
LTWAGIALALVEISPVTLQDSVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGI
VFGIIQLRLQKQGIEKAFPFGPYLAIAGWISLIWGDQILNWYFTSVLGV

Nucleotide

• Length: 870 bp

>NTDB_id=601280 K6750_RS02835 WP_017635919.1 582740..583609(-) (pilD) [Vibrio alginolyticus strain ZZV2]
ATGGAAGTATTCCAGTACTACCCTTGGCTATTCGTTGTGTTTGCCACCATCTTCGGTCTGATCGTGGGTAGCTTCCTCAA
TGTGGTTATTTATCGACTACCTAAAATCATGGAACTAGAATGGCGACGCGAATGTGCCGAGTCATTTCCTGAATACAAAA
TAGAACCTCCTAAAGAAGTGCTGACCTTGAGCGTTCCACGCTCCTCCTGCCAGAAATGCGCAACACCGATACGTATTCGA
GACAACATTCCTGTGATTAGCTGGCTGCTTTTAAAAGGCAAATGTCACAACTGTCAGGCAACCATTAGTGCACGCTATCC
ACTGATTGAACTGCTCACCGCGTTCTGTTCAGGCTTTATCGCTTATTACTTTGGTTTTAGTTACTTTACCGTTGCCCTAC
TCTTTTTTACTTTTATCTTAATCGCCGCAACATTTATCGATCTCGATACTATGTTGTTACCTGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCCTTAGCATTAGTCGAAATAAGTCCTGTTACTCTACAAGATTCTGTCATTGGTGCTATTGC
TGGTTACCTATGTCTCTGGTCCGTTTACTGGGGCTTTAAGTTACTTACTGGTAAAGAAGGAATGGGGTATGGTGATTTTA
AACTACTAGCGGCACTAGGAGCTTGGCTAGGTTGGCAGTCACTGCCGATGATCATTCTACTTTCTTCTGTGGTTGGCATC
GTATTTGGCATCATTCAACTACGCTTGCAAAAGCAGGGAATTGAAAAAGCTTTCCCTTTCGGTCCTTACTTAGCAATTGC
TGGTTGGATAAGCTTAATTTGGGGCGACCAGATTTTAAACTGGTACTTTACTTCAGTTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A2I3C9C8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	91.349	100	0.913
pilD	Vibrio cholerae strain A1552	73.264	99.654	0.73
pilD	Acinetobacter baumannii D1279779	52.222	93.426	0.488
pilD	Acinetobacter nosocomialis M2	51.852	93.426	0.484
pilD	Neisseria gonorrhoeae MS11	47.653	95.848	0.457