Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K6I41_RS17435 Genome accession   NZ_CP082274
Coordinates   3683293..3685005 (+) Length   570 a.a.
NCBI ID   WP_005301829.1    Uniprot ID   -
Organism   Acinetobacter sp. AS23     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3678293..3690005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6I41_RS17400 (K6I41_17285) nusA 3679180..3680664 (-) 1485 WP_003654584.1 transcription termination factor NusA -
  K6I41_RS17405 (K6I41_17290) rimP 3680701..3681228 (-) 528 WP_004795010.1 ribosome maturation factor RimP -
  K6I41_RS17425 (K6I41_17310) secG 3681865..3682194 (-) 330 WP_004795012.1 preprotein translocase subunit SecG -
  K6I41_RS17430 (K6I41_17315) tpiA 3682207..3683001 (-) 795 WP_250374853.1 triose-phosphate isomerase -
  K6I41_RS17435 (K6I41_17320) pilB 3683293..3685005 (+) 1713 WP_005301829.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K6I41_RS17440 (K6I41_17325) pilC 3685033..3686259 (+) 1227 WP_049837982.1 type II secretion system F family protein Machinery gene
  K6I41_RS19175 (K6I41_17330) pilD 3686259..3687119 (+) 861 WP_284453895.1 A24 family peptidase Machinery gene
  K6I41_RS17455 (K6I41_17335) coaE 3687121..3687717 (+) 597 WP_049837984.1 dephospho-CoA kinase -
  K6I41_RS17460 (K6I41_17340) - 3687714..3688628 (-) 915 WP_250374854.1 DMT family transporter -
  K6I41_RS17465 (K6I41_17345) rlmB 3688664..3689413 (-) 750 WP_004795029.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  K6I41_RS17470 (K6I41_17350) - 3689516..3689842 (-) 327 WP_004795031.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63507.87 Da        Isoelectric Point: 4.9650

>NTDB_id=600774 K6I41_RS17435 WP_005301829.1 3683293..3685005(+) (pilB) [Acinetobacter sp. AS23]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQAALDAAKRAKQDTVAYLIEEVNLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLVQRGQILYVATSNPSNIDAIDAIRFNSKLMVEPVIVEHHKLEKILVQQFTEESNFEFSDEEF
DLDVDLDTSTAPEEDEDTPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKVPTDIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=600774 K6I41_RS17435 WP_005301829.1 3683293..3685005(+) (pilB) [Acinetobacter sp. AS23]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGACGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAGCAGCACTAGATGCAGCAAAAAGAGCTAAACAGGATACTGTTGCGTACCTGATTGAAGAGGTTAATCTCTCCC
CTTCTTTATTAGCTGAGACAATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACCAGCCAGATT
CCTAAAGATTTGGTCGACCAGAAACTGATTTTAAAACATCGGATCTTGCCTCTCGTACAAAGAGGACAGATTTTATATGT
TGCAACCAGTAATCCCAGCAATATTGATGCTATTGATGCTATTCGCTTCAACAGCAAGCTTATGGTTGAGCCAGTTATTG
TCGAGCACCATAAACTCGAGAAAATTCTGGTACAGCAATTTACTGAAGAAAGTAATTTCGAGTTTAGTGATGAAGAGTTT
GATCTAGATGTAGATCTTGATACCTCAACTGCTCCAGAAGAAGATGAGGATACTCCGCAAGGCGATGAAGCACCTATTGT
TAAATATATTAACAAGTTATTGATTGATGCGATTCGTATGGGGGCTTCTGATTTACATTTCGAACCTTATGAAAAATCGT
ATCGGGTCCGTTATCGGGTGGATGGCGTTTTGCGTCAAATTGCAAATCCACCTCTGCAACTGGCTAATCGTTTGGCTTCA
CGCTTAAAAGTGATGTCTCAAATGGACATTTCTGAAAAGCGTGTCCCTCAAGATGGTCGTATCAAGCTCAAGCTATCAAA
ATCTAAAGCAATTGATTTTCGTGTGAACTCTCTTCCGACCTTATTTGGTGAAAAGCTAGTTCTACGTATTCTCGATCCAT
CGAGTGCTATGCTGGGAATTGATGCTCTAGGATATGAAGAAGAGCAAAAAGCGCTCTTTATGGAAGCATTAGATAAGCCA
CAAGGTATGCTTTTAATCACAGGTCCGACAGGTTCTGGTAAGACTGTATCTTTATATACAGGCCTCAATATCTTAAATAC
TGAAAGTTCTAATATTTCTACTGCCGAAGATCCAGTCGAAATTAACCTTGAAGGCATTAATCAGGTTAACGTGAACCCTA
AGGTAGGTCTGACTTTTTCAGCTGCACTTAAGTCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GATTTAGAAACAGCTGAAATCGCGATTAAAGCGGCTCAGACAGGTCATATGGTGATGTCCACGCTACATACCAACAGTGC
GCCTGAAACACTGACTCGTCTACGCAATATGGGGGTTCCGTCTTTCAATATTGCGACATCGGTCAATCTGGTCATTGCTC
AGCGTTTGGCACGTCGACTATGTTCTCAATGTAAAGTACCTACTGACATTCCTAAACAAAGCCTATTGGAAATGGGTTTT
ACCGAACAAGATCTGACACATCCCGATTTTCAAGTTTTTCAACCAGTCGGTTGTCCAGAATGTCGTGAGGGTTACAAAGG
TCGAGTTGGTATTTATGAAGTAATGAAAGTCACACCTGAGATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCCGCTTCTGAAAAACTGGGGTTTAATAATCTACGTCGCTCAGGTTTAAAGAAAGTTATGCAAGGTGTGACTTCT
TTACAAGAAGTCAATCGCGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

94.561

100

0.946
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

51.937

99.649

0.518
  pilF Neisseria gonorrhoeae MS11

49.11

98.596

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

51.901

92.281

0.479
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.342

100

0.381
  pilF Thermus thermophilus HB27

36.964

98.246

0.363