Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   K6978_RS13350 Genome accession   NZ_CP082214
Coordinates   3014354..3015484 (-) Length   376 a.a.
NCBI ID   WP_104604006.1    Uniprot ID   -
Organism   Xanthomonas cucurbitae strain OH_261     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3009354..3020484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K6978_RS13325 (K6978_13280) ruvX 3009843..3010310 (-) 468 WP_104605335.1 Holliday junction resolvase RuvX -
  K6978_RS13330 (K6978_13285) - 3010303..3010869 (-) 567 WP_104605333.1 YqgE/AlgH family protein -
  K6978_RS13335 (K6978_13290) - 3010947..3012800 (+) 1854 WP_104605331.1 DUF4153 domain-containing protein -
  K6978_RS13340 (K6978_13295) - 3012797..3013336 (+) 540 WP_274396372.1 DNA-3-methyladenine glycosylase I -
  K6978_RS13345 (K6978_13300) - 3013452..3014126 (-) 675 WP_170067021.1 YitT family protein -
  K6978_RS13350 (K6978_13305) pilU 3014354..3015484 (-) 1131 WP_104604006.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  K6978_RS13355 (K6978_13310) pilT 3015599..3016636 (-) 1038 WP_104604005.1 type IV pilus twitching motility protein PilT Machinery gene
  K6978_RS13360 (K6978_13315) - 3016880..3017572 (+) 693 WP_159407309.1 YggS family pyridoxal phosphate-dependent enzyme -
  K6978_RS13365 (K6978_13320) proC 3017621..3018472 (+) 852 WP_104604004.1 pyrroline-5-carboxylate reductase -
  K6978_RS13370 (K6978_13325) - 3018925..3019347 (+) 423 WP_104604003.1 HU family DNA-binding protein -
  K6978_RS13375 (K6978_13330) - 3019504..3019686 (-) 183 WP_104604002.1 hypothetical protein -
  K6978_RS13380 (K6978_13335) - 3019721..3020317 (+) 597 WP_274397262.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41923.21 Da        Isoelectric Point: 6.8287

>NTDB_id=600231 K6978_RS13350 WP_104604006.1 3014354..3015484(-) (pilU) [Xanthomonas cucurbitae strain OH_261]
MSSIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPAQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGAGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=600231 K6978_RS13350 WP_104604006.1 3014354..3015484(-) (pilU) [Xanthomonas cucurbitae strain OH_261]
ATGAGCAGCATCGACTTCACCTCTTTTCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCCGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACACCGCTGACCGCGCAGCAGAGCCGCGACCTGG
TGCTGAACGTGATGACGCCAGCGCAGCGCGAGGAGTTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGTGTGTCGGGC
GTGGGCCGATTCCGTGTGAGCTGCTTCTATCAACGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATTCC
CACCGTCGAAGAACTGAGCCTGCCGCCGGTCATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTGCCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGCGAAGTCGGCATCGACACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTCATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAATGCCAACCAGGCGATG
GATCGCATCATCAACTTCTTCCCGGAGGATCGTCGCAGCCAGTTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCCCAGCAGCTGATCCCCACCCCGGATGGGCGCAGCCGCCGCGTGGCGATGGAAATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGTGATGGTGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAGATCAGCTACGAAGATGCCTTGCGTTATGCCGATTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGGGATGCCAAGACCCTGGCGCAGGGACTGGACGGGGTGGAGATCGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.485

97.606

0.649
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364