Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5Q02_RS00725 Genome accession   NZ_CP081942
Coordinates   167155..168861 (+) Length   568 a.a.
NCBI ID   WP_225835414.1    Uniprot ID   -
Organism   Pseudomonas sp. MM211     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 162155..173861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5Q02_RS00710 (K5Q02_00705) tfpZ 163803..164537 (-) 735 WP_225835402.1 TfpX/TfpZ family type IV pilin accessory protein -
  K5Q02_RS00715 (K5Q02_00710) - 165025..166398 (-) 1374 WP_225835405.1 O-antigen ligase family protein -
  K5Q02_RS00720 (K5Q02_00715) pilA/pilAI 166477..166896 (-) 420 WP_225835412.1 pilin Machinery gene
  K5Q02_RS00725 (K5Q02_00720) pilB 167155..168861 (+) 1707 WP_225835414.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5Q02_RS00730 (K5Q02_00725) pilC 168864..170084 (+) 1221 WP_225835416.1 type II secretion system F family protein Machinery gene
  K5Q02_RS00735 (K5Q02_00730) pilD 170088..170960 (+) 873 WP_225835417.1 A24 family peptidase Machinery gene
  K5Q02_RS00740 (K5Q02_00735) coaE 171111..171719 (+) 609 WP_225835418.1 dephospho-CoA kinase -
  K5Q02_RS00745 (K5Q02_00740) yacG 171716..171916 (+) 201 WP_225835420.1 DNA gyrase inhibitor YacG -
  K5Q02_RS00750 (K5Q02_00745) - 171972..172478 (+) 507 WP_225835422.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62513.41 Da        Isoelectric Point: 5.6172

>NTDB_id=598545 K5Q02_RS00725 WP_225835414.1 167155..168861(+) (pilB) [Pseudomonas sp. MM211]
MSENIPLSGLAKQVVLAELLDEKAAQQAQAQAKRNKLSLVTYLAQNKLVKSRALAELAADQFGVAYFDLSALDKESQPKD
LVSEKLVRQHRTLPLWRRGNKLFVGISDPSNHQAVTDIQFSTGLSTEALLVEDDKLGDAIDKFFDTAHSGLDDLGDVDLD
GLETESVEDDKKINDGSGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGILHETARPPIQLAPRISA
RLKVMAGLDISERRKPQDGRIKMKISKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEESQKDLYMAALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIR
DLDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNSCKKEIPIPDEALLEEGF
PQDKLGTFKIYGPVGCENCKNGYKGRVGIYEVVKNTPSLQRIIMEEGNSIDISIQMRKDGFNDLRTSALLKAMQGVTSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=598545 K5Q02_RS00725 WP_225835414.1 167155..168861(+) (pilB) [Pseudomonas sp. MM211]
ATGAGCGAGAACATTCCACTTTCCGGCCTGGCCAAGCAGGTGGTACTGGCTGAGCTGTTGGACGAGAAGGCCGCTCAGCA
GGCGCAAGCGCAGGCCAAGCGCAATAAACTGTCGCTGGTCACCTACCTTGCACAGAACAAACTGGTAAAGAGTCGCGCGC
TCGCGGAGCTAGCCGCAGATCAGTTTGGCGTAGCTTATTTCGACCTCAGCGCACTGGACAAGGAAAGTCAGCCCAAGGAT
CTAGTCAGCGAAAAGCTTGTCCGCCAGCATCGAACATTGCCCCTTTGGCGGCGTGGCAACAAGTTGTTCGTAGGTATATC
AGACCCGTCAAATCATCAAGCCGTCACGGACATTCAGTTCAGTACTGGCCTTTCCACGGAAGCGCTGCTGGTCGAGGATG
ACAAGCTGGGCGATGCCATCGATAAGTTCTTCGATACCGCCCATAGCGGCTTGGACGATCTGGGCGATGTAGATCTCGAT
GGCCTAGAGACCGAGTCGGTCGAAGATGACAAGAAGATCAACGATGGCAGCGGCCAGGATGCTGATGATGCGCCAGTGGT
GCGCTTCGTCAATAAAATGCTGCTCGATGCGATCAAAGGCGGCTCATCCGATCTCCACTTCGAACCCTATGAAAAAGCTT
ACCGCGTGCGTTTTCGTACCGACGGCATTCTGCATGAGACGGCACGCCCGCCGATTCAGTTGGCACCGCGTATATCTGCA
CGTTTGAAGGTTATGGCTGGCCTGGACATCTCCGAACGGCGCAAGCCGCAGGACGGCCGTATAAAGATGAAAATATCCAA
AAGCAAGTCCATCGATTTCCGCGTCAACACTCTGCCCACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGATTCCT
CCAGCGCGCAAATGGGGATCGATGCCCTGGGCTACGAGGAAAGCCAGAAAGACCTATATATGGCTGCACTCAAGCAACCA
CAGGGAATGATTCTGGTAACGGGCCCAACAGGGTCTGGCAAAACGGTCTCTCTCTACACTGGCCTCAACATCCTCAATAC
CCCGGACGTGAATATTTCCACGGCGGAAGATCCGGTGGAGATCAACCTGGAAGGGATCAATCAGGTCAACGTCAACCCCA
AGCAGGGCATGGACTTCACTCAGGCACTGCGTGCGTTCCTGCGCCAAGACCCTGACATCATCATGGTCGGCGAGATTCGC
GACTTGGACACGGCCTCCATTGCTATCAAGGCCGCACAGACCGGCCATATGGTGATGTCGACCCTGCACACCAACAGCGC
AGCGGAAACCCTGACCCGTTTGCGCAATATGGGCGTGCCCTCCTTCAACATCGCCACCTCGGTAAACCTGATCATTGCCC
AGCGCTTGGCGCGTAAGCTCTGCAATAGCTGCAAGAAAGAAATACCGATTCCTGACGAGGCTTTGCTGGAGGAAGGTTTC
CCCCAGGACAAGCTTGGAACCTTCAAAATTTACGGCCCGGTTGGCTGTGAAAATTGCAAGAACGGTTACAAAGGTCGTGT
CGGTATTTATGAAGTGGTTAAAAACACGCCGAGCCTGCAGAGGATTATCATGGAGGAAGGCAACTCCATCGATATTTCCA
TACAGATGCGAAAAGACGGCTTCAACGACCTGCGTACTTCAGCGCTGCTGAAGGCCATGCAAGGCGTTACCAGCCTTGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.649

100

0.599
  pilB Acinetobacter baylyi ADP1

57.746

100

0.577
  pilB Legionella pneumophila strain ERS1305867

55.323

100

0.558
  pilB Vibrio parahaemolyticus RIMD 2210633

51.391

94.894

0.488
  pilB Vibrio campbellii strain DS40M4

49.726

96.303

0.479
  pilB Vibrio cholerae strain A1552

49.091

96.831

0.475
  pilF Neisseria gonorrhoeae MS11

47.518

99.296

0.472
  pilF Thermus thermophilus HB27

36.268

100

0.363