Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NBY12_RS04245 Genome accession   NZ_CP098223
Coordinates   879287..880672 (-) Length   461 a.a.
NCBI ID   WP_032302388.1    Uniprot ID   -
Organism   Escherichia coli strain Z0117EC0005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 874287..885672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY12_RS04210 (NBY12_04215) mutT 874622..875020 (+) 399 WP_000736034.1 8-oxo-dGTP diphosphatase MutT -
  NBY12_RS04215 (NBY12_04220) yacG 875222..875419 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  NBY12_RS04220 (NBY12_04225) zapD 875429..876172 (-) 744 WP_001194743.1 cell division protein ZapD -
  NBY12_RS04225 (NBY12_04230) coaE 876172..876792 (-) 621 WP_001269517.1 dephospho-CoA kinase -
  NBY12_RS04230 (NBY12_04235) - 876817..876861 (+) 45 WP_120795372.1 protein YacM -
  NBY12_RS04235 (NBY12_04240) guaC 877017..878060 (+) 1044 WP_001217338.1 GMP reductase -
  NBY12_RS04240 (NBY12_04245) hofC 878095..879297 (-) 1203 WP_032302387.1 protein transport protein HofC -
  NBY12_RS04245 (NBY12_04250) pilB 879287..880672 (-) 1386 WP_032302388.1 type II secretion system protein GspE Machinery gene
  NBY12_RS04250 (NBY12_04255) pilA 880682..881122 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NBY12_RS04255 (NBY12_04260) nadC 881325..882218 (-) 894 WP_032183211.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NBY12_RS04260 (NBY12_04265) ampD 882306..882857 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NBY12_RS04265 (NBY12_04270) ampE 882854..883708 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NBY12_RS04270 (NBY12_04275) aroP 883751..885124 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50666.39 Da        Isoelectric Point: 6.4549

>NTDB_id=598487 NBY12_RS04245 WP_032302388.1 879287..880672(-) (pilB) [Escherichia coli strain Z0117EC0005]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCQGGEKVVLRLLQQVSQALDINTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRLQQGEPIHIPDNVWPSPLPRWQAPGCVHCYHGFYGRTALFE
VLPITPFIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=598487 NBY12_RS04245 WP_032302388.1 879287..880672(-) (pilB) [Escherichia coli strain Z0117EC0005]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACCCAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGTGTATTGCATCCTTTACCGGATGTTTTACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGCAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGCCAGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATATCAACACGCTGGGGATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGTCTTACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACAGCAGAGATCGCTATTAAAGCGGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTCTCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCTGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCGCTGGCAAGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGTTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGAATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.505

100

0.419
  pilB Legionella pneumophila strain ERS1305867

50

82.863

0.414
  pilB Vibrio campbellii strain DS40M4

48.705

83.731

0.408
  pilB Haemophilus influenzae 86-028NP

39.915

100

0.408
  pilB Vibrio cholerae strain A1552

47.179

84.599

0.399
  pilB Vibrio parahaemolyticus RIMD 2210633

46.923

84.599

0.397
  pilB Acinetobacter baylyi ADP1

45.478

83.948

0.382
  pilB Acinetobacter baumannii D1279779

44.33

84.165

0.373
  pilF Neisseria gonorrhoeae MS11

44.675

83.514

0.373
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilF Thermus thermophilus HB27

41.687

87.419

0.364