Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NBY12_RS04250 Genome accession   NZ_CP098223
Coordinates   880682..881122 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain Z0117EC0005     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 875682..886122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NBY12_RS04225 (NBY12_04230) coaE 876172..876792 (-) 621 WP_001269517.1 dephospho-CoA kinase -
  NBY12_RS04230 (NBY12_04235) - 876817..876861 (+) 45 WP_120795372.1 protein YacM -
  NBY12_RS04235 (NBY12_04240) guaC 877017..878060 (+) 1044 WP_001217338.1 GMP reductase -
  NBY12_RS04240 (NBY12_04245) hofC 878095..879297 (-) 1203 WP_032302387.1 protein transport protein HofC -
  NBY12_RS04245 (NBY12_04250) pilB 879287..880672 (-) 1386 WP_032302388.1 type II secretion system protein GspE Machinery gene
  NBY12_RS04250 (NBY12_04255) pilA 880682..881122 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  NBY12_RS04255 (NBY12_04260) nadC 881325..882218 (-) 894 WP_032183211.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NBY12_RS04260 (NBY12_04265) ampD 882306..882857 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NBY12_RS04265 (NBY12_04270) ampE 882854..883708 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  NBY12_RS04270 (NBY12_04275) aroP 883751..885124 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=598488 NBY12_RS04250 WP_000360904.1 880682..881122(-) (pilA) [Escherichia coli strain Z0117EC0005]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=598488 NBY12_RS04250 WP_000360904.1 880682..881122(-) (pilA) [Escherichia coli strain Z0117EC0005]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTTCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363