Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K4A76_RS22175 Genome accession   NZ_CP081491
Coordinates   5000062..5001330 (-) Length   422 a.a.
NCBI ID   WP_274411148.1    Uniprot ID   -
Organism   Pseudomonas sp. NEEL19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4995062..5006330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K4A76_RS22160 (K4A76_22065) - 4996739..4998328 (-) 1590 WP_054881140.1 AAA family ATPase -
  K4A76_RS22165 (K4A76_22070) aroB 4998344..4999441 (-) 1098 WP_274411147.1 3-dehydroquinate synthase -
  K4A76_RS22170 (K4A76_22075) aroK 4999539..5000057 (-) 519 WP_009684951.1 shikimate kinase AroK -
  K4A76_RS22175 (K4A76_22080) pilQ 5000062..5001330 (-) 1269 WP_274411148.1 type IV pilus secretin PilQ family protein Machinery gene
  K4A76_RS22180 (K4A76_22085) - 5001254..5002252 (-) 999 WP_274412319.1 pilus assembly protein PilP -
  K4A76_RS22185 (K4A76_22090) - 5002264..5002800 (-) 537 WP_274411149.1 PilN domain-containing protein -
  K4A76_RS22190 (K4A76_22095) pilM 5002797..5003678 (-) 882 WP_274411150.1 pilus assembly protein PilM -
  K4A76_RS22195 (K4A76_22100) - 5003857..5006310 (+) 2454 WP_182389444.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 46026.76 Da        Isoelectric Point: 4.9166

>NTDB_id=596984 K4A76_RS22175 WP_274411148.1 5000062..5001330(-) (pilQ) [Pseudomonas sp. NEEL19]
MRTRMIYLLAALLPMPLATATPYQGEPLSLHFQDVEIRSVLQVLADYAGVNLVASDDVQGNVTLRLQDVPWDQALDLVLR
SKSLARRQEGNVLLVAPAATLAAQTAEARAGQVLEAQFQPMRRELLPVHHAKAAELAELLKTSLADESQLAGWGSLSVDE
RTNTLVAYLPAERLDELRQLVAQLDVPVRQVAIEARIVEANVDYEKALGVRWGSPLYGDNPLPGKSLFVDLGVERAGSSI
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETTKSGATSVSFREASLSLEVTPQITPDN
KVIMAVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFLGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=596984 K4A76_RS22175 WP_274411148.1 5000062..5001330(-) (pilQ) [Pseudomonas sp. NEEL19]
ATGAGAACGAGGATGATCTACCTGCTGGCGGCCCTGCTGCCCATGCCTCTGGCGACCGCGACGCCTTACCAGGGTGAGCC
GCTGTCGCTGCACTTTCAGGATGTCGAGATTCGCTCGGTTTTGCAGGTACTCGCCGACTATGCCGGCGTCAACCTGGTCG
CCAGCGACGACGTGCAGGGCAATGTCACCCTGCGCTTGCAGGATGTGCCGTGGGATCAGGCGCTGGACCTGGTCTTGCGC
AGCAAAAGCCTCGCCCGGCGCCAGGAGGGCAATGTGCTGCTGGTGGCGCCGGCGGCCACGCTGGCTGCGCAAACCGCCGA
AGCGCGTGCGGGGCAGGTGCTGGAGGCGCAGTTCCAGCCCATGCGCCGGGAGCTGTTGCCTGTTCACCATGCCAAGGCTG
CCGAACTTGCGGAACTGCTCAAGACCAGCCTGGCCGACGAAAGCCAGCTCGCCGGTTGGGGCAGCCTGAGTGTCGATGAG
CGCACCAATACCCTGGTGGCCTACCTGCCGGCCGAACGCCTCGATGAACTGCGGCAACTGGTGGCGCAGCTGGATGTGCC
GGTGCGTCAGGTGGCGATCGAGGCGCGCATCGTCGAGGCCAACGTCGATTACGAGAAAGCCCTTGGGGTGCGCTGGGGCA
GCCCTTTGTATGGCGACAACCCGCTGCCGGGCAAGTCGCTGTTCGTCGACCTGGGTGTCGAGCGGGCTGGCAGCAGCATC
GGCCTTGGCCTGCTGCGCGGGGATGTGCTGCTGGACCTCGAGCTCAGCGCCATGGAAAAAAGCGGCAATGGCGAGATCAT
TTCGCAACCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAGGGCACCGAGGTGCCCTACCAGGAAACCA
CCAAGAGCGGCGCTACTTCGGTGTCGTTCCGCGAGGCCTCGCTGTCGTTGGAGGTGACCCCGCAAATTACCCCGGACAAC
AAGGTAATCATGGCTGTCAGGGTGACCAAGGACGAACCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTGAATGCCAAGGTTCGCGTGGCCGACGGCGAAACGATCGTGATTGGCGGCGTGTACTCGACCTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTGGGTGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(50-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.404

100

0.614
  pilQ Acinetobacter baumannii strain A118

41.649

100

0.455
  pilQ Acinetobacter baumannii D1279779

41.649

100

0.455
  comQ Acinetobacter baylyi ADP1

42.762

100

0.455
  pilQ Legionella pneumophila strain ERS1305867

42.105

99.052

0.417
  pilQ Legionella pneumophila strain Lp02

42.105

99.052

0.417
  pilQ Vibrio campbellii strain DS40M4

41.274

100

0.415
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.625

98.578

0.4
  pilQ Vibrio cholerae strain A1552

40.625

98.578

0.4