Detailed information NTG0596443

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: A237_RS04345
• Genome accession: NZ_CP081457
• Coordinates: 941159..942376 (-)
• Length: 405 a.a.
• NCBI ID: WP_005614794.1
• Uniprot ID: A0A3M4KPU8
• Organism: Pseudomonas syringae pv. actinidifoliorum ICMP 18803
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	922076..948714	941159..942376	within	0

Gene organization within MGE regions

Location: 922076..948714

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A237_RS04275 (A237_004255)	-	922076..922687 (+)	612	WP_017700649.1	hypothetical protein	-
A237_RS04280 (A237_004260)	-	922883..924283 (+)	1401	WP_017700650.1	TolC family outer membrane protein	-
A237_RS04285 (A237_004265)	-	924430..926592 (+)	2163	WP_017700651.1	type I secretion system permease/ATPase	-
A237_RS04290 (A237_004270)	-	926585..928006 (+)	1422	WP_017700652.1	HlyD family type I secretion periplasmic adaptor subunit	-
A237_RS04295 (A237_004275)	-	928068..929573 (+)	1506	WP_017700653.1	tetratricopeptide repeat protein	-
A237_RS04300 (A237_004280)	-	929809..934365 (+)	4557	WP_264297527.1	calcium-binding protein	-
A237_RS04305 (A237_004285)	-	934316..934855 (+)	540	WP_264297528.1	calcium-binding protein	-
A237_RS04310 (A237_004290)	-	934806..935312 (+)	507	WP_264297529.1	calcium-binding protein	-
A237_RS04315	-	935297..936940 (+)	1644	WP_264297530.1	calcium-binding protein	-
A237_RS04320 (A237_004310)	tnpB	937063..937422 (+)	360	WP_005621010.1	IS66 family insertion sequence element accessory protein TnpB	-
A237_RS04325 (A237_004315)	tnpC	937440..938972 (+)	1533	WP_032607211.1	IS66 family transposase	-
A237_RS04330 (A237_004320)	yacG	939459..939668 (-)	210	WP_017700940.1	DNA gyrase inhibitor YacG	-
A237_RS04335 (A237_004325)	coaE	939665..940288 (-)	624	WP_017700941.1	dephospho-CoA kinase	-
A237_RS04340 (A237_004330)	pilD	940285..941157 (-)	873	WP_017700942.1	A24 family peptidase	Machinery gene
A237_RS04345 (A237_004335)	pilC	941159..942376 (-)	1218	WP_005614794.1	type II secretion system F family protein	Machinery gene
A237_RS04350 (A237_004340)	pilB	942379..944073 (-)	1695	WP_017700943.1	type IV-A pilus assembly ATPase PilB	Machinery gene
A237_RS04355 (A237_004345)	-	944299..944685 (+)	387	WP_020320879.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
A237_RS04360 (A237_004350)	-	945171..946358 (+)	1188	WP_375233760.1	O-antigen ligase family protein	-
A237_RS04365 (A237_004355)	-	946467..947753 (+)	1287	WP_017700945.1	polysaccharide biosynthesis C-terminal domain-containing protein	-
A237_RS28115 (A237_004360)	-	947679..947858 (-)	180	WP_077528389.1	DUF1523 family protein	-
A237_RS04375 (A237_004365)	-	948127..948714 (+)	588	WP_017700946.1	hypothetical protein	-

Sequence

Protein

• Length: 405 a.a.
• Molecular weight: 43893.71 Da
• Isoelectric Point: 10.1097

>NTDB_id=596443 A237_RS04345 WP_005614794.1 941159..942376(-) (pilC) [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
MASKAVKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPIKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVNSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
ERLKAKIKKAMTYPAAVLIVAIIVSGILLIKVVPQFQTVFAGFGAQLPAFTLMVIGLSGFVEQWWLAIIGLFFAGIFLFK
RAYKASQNFRDSVDRFLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFT
LGNAV

Nucleotide

• Length: 1218 bp

>NTDB_id=596443 A237_RS04345 WP_005614794.1 941159..942376(-) (pilC) [Pseudomonas syringae pv. actinidifoliorum ICMP 18803]
ATGGCCAGTAAGGCAGTTAAAGTCATCGTTTACACTTGGGAAGGTGTCGACAAAAAGGGCACCAAAACCAGCGGCGAATT
GAGCGGCCATAACTTGGCACTCGTAAAAGCCCAGTTGCGCAAGCAAGGCATCAATCCGATTAAGGTGCGCAAGAAATCCG
TATCGATCTTCGGCAAGGGAAAAAAAATCAAGCCTCTGGATATAGCTTTTTTTTCTAGGCAAATGGCAACGATGATGAAA
GCAGGTGTGCCCCTGCTTCAGTCATTCGATATCATCAGCGAAGGTGCCGAGAACCCTAACATGCGCGCACTGGTCAACTC
GCTCAAACAAGAAGTTTCTGCAGGCAACAGCTTTGCAACAGCCTTACGACAAAAACCTGAGTACTTCGACGACCTATTTT
GCAATCTAGTAGATGCCGGCGAACAAGCCGGAGCTCTTGAAAGCCTGCTAGACCGTGTCGCAAGCTATAAGGAAAAGACA
GAAAGACTTAAGGCAAAAATCAAAAAAGCAATGACCTATCCCGCAGCCGTGTTGATAGTAGCGATTATAGTTTCAGGAAT
TTTGCTTATAAAGGTAGTGCCACAATTTCAGACTGTGTTCGCTGGCTTTGGCGCGCAGCTGCCTGCATTTACACTAATGG
TCATCGGGCTTTCAGGCTTTGTGGAGCAATGGTGGCTGGCCATTATCGGTTTATTCTTCGCAGGTATTTTTTTGTTCAAA
CGAGCCTACAAGGCGTCACAAAACTTCCGTGACAGCGTTGATCGTTTTTTGTTGAAAGTTCCGATCATTGGACCCTTGAT
TTTCAAGTCATCTGTAGCTCGGTACGCCCGCACTCTTGCAACGACTTTTGCTGCAGGCGTGCCATTGGTCGAAGCTTTGG
ACTCCGTAGCAGGCGCCACTGGGAACGTGGTTTTCAAAAATGCTGTTAACAAGGTCAAGCAAGATGTGTCCACGGGTATG
CAATTGAATTTTTCCATGCGCTCGACGGGTGTCTTCCCCAGCTTGGCGATCCAGATGACTGCGATCGGCGAAGAGTCTGG
TGCGCTGGACACCATGCTGGATAAAGTCGCAACCTATTATGAAGATGAGGTTGATAACATGGTCGACAGTTTGACTAGTC
TGATGGAACCAATGATCATGGCGGTGCTGGGAGTTATAGTGGGCGGTCTTGTAATCGCCATGTATCTCCCCATTTTCACA
CTTGGAAATGCAGTCTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (71-194)

• Type II secretion system (T2SS), protein F (T2SSF) (276-397)

3D structure

• AlphaFold DB: A0A3M4KPU8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	73.333	100	0.733
pilC	Acinetobacter baylyi ADP1	59.804	100	0.602
pilC	Acinetobacter baumannii D1279779	59.406	99.753	0.593
pilC	Legionella pneumophila strain ERS1305867	50.985	100	0.511
pilG	Neisseria gonorrhoeae MS11	43.325	98.025	0.425
pilG	Neisseria meningitidis 44/76-A	43.325	98.025	0.425
pilC	Vibrio campbellii strain DS40M4	42.424	97.778	0.415
pilC	Vibrio cholerae strain A1552	41.646	99.012	0.412
pilC	Thermus thermophilus HB27	38.903	99.012	0.385