Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABEKA_RS06740 Genome accession   NZ_CP080636
Coordinates   1125294..1127003 (-) Length   569 a.a.
NCBI ID   WP_222110694.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain EK30A     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1120294..1132003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEKA_RS06710 (ABEKA_1094) - 1120414..1120737 (+) 324 WP_004281089.1 pyrimidine/purine nucleoside phosphorylase -
  ABEKA_RS06715 (ABEKA_1095) rlmB 1120872..1121621 (+) 750 WP_005101440.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ABEKA_RS06720 (ABEKA_1096) - 1121625..1122551 (+) 927 WP_222110690.1 DMT family transporter -
  ABEKA_RS06725 (ABEKA_1097) coaE 1122572..1123171 (-) 600 WP_222110691.1 dephospho-CoA kinase -
  ABEKA_RS17320 (ABEKA_1098) pilD 1123173..1124033 (-) 861 WP_222110692.1 A24 family peptidase Machinery gene
  ABEKA_RS06735 (ABEKA_1099) pilC 1124033..1125262 (-) 1230 WP_222110693.1 type II secretion system F family protein Machinery gene
  ABEKA_RS06740 (ABEKA_1100) pilB 1125294..1127003 (-) 1710 WP_222110694.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABEKA_RS06745 (ABEKA_1101) tpiA 1127280..1128071 (+) 792 WP_005094392.1 triose-phosphate isomerase -
  ABEKA_RS06750 (ABEKA_1102) secG 1128085..1128414 (+) 330 WP_222110695.1 preprotein translocase subunit SecG -
  ABEKA_RS06780 (ABEKA_1108) rimP 1129483..1130007 (+) 525 WP_222110696.1 ribosome maturation factor RimP -
  ABEKA_RS06785 (ABEKA_1109) nusA 1130042..1131526 (+) 1485 WP_004281105.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63512.70 Da        Isoelectric Point: 4.8339

>NTDB_id=594620 ABEKA_RS06740 WP_222110694.1 1125294..1127003(-) (pilB) [Acinetobacter lwoffii strain EK30A]
MTALQGSPRFTGFIRRLVEEGVISAEDMRSALAHAKQEKIDIVAELINQQQLSPTVIAETISVEFGEPLFDISAYDPAQI
LRDAIDEKLITKHRILPIFKNSSILYVATSNPTNIEAIDAVRFSTKLNIETIIVEHNKLEKLIEQNFTEESTFEFDEDFD
LDVDVESADPNKEEDDSNKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKIYRVRYRVDGVLRQIATPPLQLANRLSSR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPKQSLLEMGFT
ETDLQQPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNALEIADASARAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=594620 ABEKA_RS06740 WP_222110694.1 1125294..1127003(-) (pilB) [Acinetobacter lwoffii strain EK30A]
ATGACAGCATTACAGGGGTCGCCAAGATTTACCGGATTTATTCGTCGTTTGGTCGAGGAAGGCGTCATCTCGGCTGAAGA
TATGCGCAGTGCTTTGGCACATGCCAAGCAAGAAAAAATAGATATTGTGGCGGAGCTGATTAACCAGCAGCAGCTTTCTC
CGACCGTCATTGCTGAAACGATTTCAGTTGAATTTGGCGAACCGCTGTTTGATATCAGTGCCTATGATCCTGCCCAAATT
TTGCGGGATGCGATTGATGAAAAGCTGATTACCAAGCATCGCATCCTGCCAATTTTTAAAAATTCCAGTATTTTATATGT
TGCGACCAGTAACCCCACCAATATCGAAGCAATTGATGCAGTCCGTTTTTCTACCAAACTCAATATTGAAACCATTATTG
TTGAACACAATAAACTTGAAAAACTGATCGAGCAGAATTTTACCGAAGAAAGCACCTTTGAGTTTGATGAAGACTTTGAT
CTGGATGTTGATGTCGAATCTGCTGATCCGAATAAAGAAGAGGATGACAGTAACAAGGGTGAAGAAGCCCCTATTGTTAA
ATATATTAATAAATTGCTGATTGATGCGATCCGTATGGGGGCTTCAGACTTACACTTTGAACCTTATGAAAAAATCTATC
GGGTACGTTACCGGGTCGATGGGGTGCTACGTCAGATTGCAACCCCGCCCTTACAGCTGGCAAATCGCCTGTCTTCGCGT
CTTAAGGTTATGTCGCAAATGGACATTTCGGAAAAGCGTGTACCTCAAGATGGTCGTATTAAGTTAAAACTTTCTAAGAA
TAAAGCCATTGATTTCCGTGTTAACTCTCTCCCTACCCTGTTTGGCGAGAAGCTGGTACTGCGTATTCTGGATCCATCCA
GCGCGATGCTGGGGATTGATGCTCTGGGCTATGAACCGGAACAAAAAGAACTGTTTATGCAGGCGCTGGACAAACCGCAA
GGGATGCTCCTAATCACTGGCCCGACAGGTTCTGGTAAAACAGTTTCTCTTTATACCGGTCTGAATATTTTAAACCGTGA
AGATACCAATATTTCGACAGCGGAAGATCCGGTCGAGATTAACTTACAAGGGATTAATCAGGTCAACGTCAATAATAAAG
TTGGCCTGACCTTCTCTGCGGCACTAAAGTCCTTCTTACGTCAAGACCCGGATATCGTCATGGTCGGTGAGATCCGGGAT
CTGGAAACTGCCGAGATTGCGATTAAAGCTGCGCAGACCGGTCATATGGTGATGTCGACGCTGCATACCAATAGTGCGCC
TGAGACTTTGACCCGTTTACGCAATATGGGGGTGCCTTCTTTCAATATTGCCACTTCAGTCAACCTGGTCATTGCACAAC
GGTTGGCACGTCGTTTGTGTTCACAATGTAAAAAACCTGCCGATATTCCGAAACAAAGCTTACTGGAGATGGGTTTTACC
GAAACCGACTTGCAGCAACCTGAGTTCCAAATTTATGAACCGGTGGGCTGCAATGAATGCCGTGAAGGCTATAAGGGTCG
TGTAGGTATTTATGAAGTTATGAAAGTAACACCTGAAATTTCCAGAATTATTATGGAGGATGGCAATGCACTTGAAATTG
CCGATGCTTCCGCACGTGCAGGTTTTAACAATTTACGCCGGTCAGGTTTAATCAAGGTGATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTCACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.526

100

0.807
  pilB Acinetobacter baylyi ADP1

76.667

100

0.768
  pilB Legionella pneumophila strain ERS1305867

51.59

99.473

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

49.195

98.243

0.483
  pilF Neisseria gonorrhoeae MS11

48.485

98.594

0.478
  pilB Vibrio campbellii strain DS40M4

48.566

98.067

0.476
  pilB Vibrio cholerae strain A1552

50.965

91.037

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

35.4

100

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

35.932

100

0.373
  pilF Thermus thermophilus HB27

37.345

99.297

0.371