Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K0H81_RS17885 Genome accession   NZ_CP080417
Coordinates   4139489..4141195 (+) Length   568 a.a.
NCBI ID   WP_220059207.1    Uniprot ID   -
Organism   Shewanella halotolerans strain FJAT-53555     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4134489..4146195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H81_RS17865 (K0H81_17865) ampE 4135256..4136107 (-) 852 WP_220059203.1 beta-lactamase regulator AmpE -
  K0H81_RS17870 (K0H81_17870) ampD 4136164..4136709 (-) 546 WP_220059204.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K0H81_RS17875 (K0H81_17875) nadC 4137156..4138037 (+) 882 WP_220059205.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0H81_RS17880 (K0H81_17880) - 4138975..4139385 (+) 411 WP_220059206.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K0H81_RS17885 (K0H81_17885) pilB 4139489..4141195 (+) 1707 WP_220059207.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0H81_RS17890 (K0H81_17890) pilC 4141222..4142487 (+) 1266 WP_220059208.1 type II secretion system F family protein Machinery gene
  K0H81_RS17895 (K0H81_17895) pilD 4142545..4143456 (+) 912 WP_220059209.1 A24 family peptidase Machinery gene
  K0H81_RS17900 (K0H81_17900) coaE 4143477..4144082 (+) 606 WP_220059210.1 dephospho-CoA kinase -
  K0H81_RS17905 (K0H81_17905) zapD 4144091..4144825 (+) 735 WP_220059211.1 cell division protein ZapD -
  K0H81_RS17910 (K0H81_17910) yacG 4144874..4145086 (+) 213 WP_041407493.1 DNA gyrase inhibitor YacG -
  K0H81_RS17915 (K0H81_17915) mutT 4145087..4145476 (+) 390 WP_220059212.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62884.88 Da        Isoelectric Point: 4.9897

>NTDB_id=592746 K0H81_RS17885 WP_220059207.1 4139489..4141195(+) (pilB) [Shewanella halotolerans strain FJAT-53555]
MPTTGLHLGLSTLFIRKQLLSEEQISSAITKSRQSKQSLVSTIVSEKLISAREIAELCYEEYGTPLLDLNEFDISGIPED
FINKKLIEKHKCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLTVALEKVLEEDISALDLDGIDEDS
LSGIEITDTDKRQEEQGDASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLAGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRALYEEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECRHPEEVPEHELINLGFT
QAQIDNGFTVYKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKEQGMRDLRESGLRKVIAGVTSIA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=592746 K0H81_RS17885 WP_220059207.1 4139489..4141195(+) (pilB) [Shewanella halotolerans strain FJAT-53555]
ATGCCAACAACTGGTCTGCATCTCGGTCTATCAACGTTATTTATTCGCAAACAATTGTTAAGCGAAGAGCAAATTTCATC
AGCAATAACCAAATCTCGCCAGTCAAAACAATCTTTAGTTTCAACCATAGTATCTGAAAAGCTTATTTCAGCTAGAGAGA
TAGCAGAGCTATGCTATGAGGAGTACGGTACTCCCCTACTCGATCTTAACGAATTTGATATCAGTGGTATTCCAGAAGAC
TTCATCAACAAAAAGTTAATTGAAAAACATAAGTGTCTTCCATTATTCAAGCGTGGAAACCGTCTGTATATAGGGACTTC
CGATCCTACCAATATTGCAGCATTAGAAGACTTTCAATTTAGTGCAGGGTTACATGCGGAGGCGATTCTCGTTGAAGATG
ACAAACTAACAGTCGCTCTTGAGAAGGTGTTAGAAGAAGATATCTCTGCGCTCGATTTAGATGGCATAGATGAAGATTCA
CTATCCGGCATTGAAATTACAGATACCGACAAGCGCCAGGAAGAACAAGGCGATGCTAGTGACGATGCTCCTATCGTCAT
CTATATCAATAAAATCCTCACAGATGCCATACGAAAGGGTGCTTCTGACTTACACTTTGAGCCATATGAAAAACGCTATC
GCATTAGATTTCGTATTGACGGCATTCTACACGAAGTCTCTGAACCCCCCGTTAATTTAGCAGGACGTATCTCCGCGCGT
CTAAAAGTAATGTCTAAGCTCGATATTGCAGAGCGCCGCGTCCCTCAAGATGGTCGAATCAAGATGAAACTGTCTCGCAC
AAAGTCTATCGACTTTCGTGTCAGCACCTTGCCCACCATCTGGGGTGAAAAAATCGTAATGCGTATCTTGGACTCATCTT
CTGCACAGCTAGGTATCGAAAAGCTCGGATATGAAGATGACCAAAGGGCACTGTACGAGGAAATGTTAGCCAAACCGCAA
GGAATGATCTTAGTCACAGGGCCAACAGGTTCGGGAAAAACGGTCTCCCTTTACACTGGACTCAATATCCTAAATACAGA
AGAACGCAACATATCTACAGCAGAAGATCCTGTTGAAATAAACTTGGAAGGCGTGAACCAAGTTCACATTAACCTAAAAG
CAGGCCTTACTTTCGCCTCAGCTCTACGCTCTTTCTTACGTCAAGATCCAGATGTGGTGATGGTGGGTGAGATACGTGAT
TTAGAAACGGCAGAAATTGCGATCAAGGCTGCTCAAACGGGTCACTTGGTATTATCAACACTCCATACCAACTCCGCCGC
AGAAACCCTCACACGTCTGATCAATATGGGGGTCCCCGGATATAACATTGCTAGCTCAGTCAACCTGATTATTGCTCAAC
GTCTCGCCCGAAGACTCTGCACCGAATGTCGTCACCCAGAAGAAGTACCGGAACATGAACTAATCAATTTGGGCTTTACC
CAGGCTCAAATTGATAATGGATTCACTGTTTATAAACCCGTTGGCTGTGACCTCTGCTCCGGTGGATACAAAGGCCGCGT
GGGTATTTACGAAGTAATGAAGATGTCAGATGAAATTGCCCGTACTATTATGGAAGGAGGCAATTCACTGCAAATAGCCA
GCCAAGCAAAAGAACAAGGCATGCGAGATCTTAGAGAGTCTGGATTACGCAAGGTCATTGCAGGCGTGACGAGTATCGCC
GAAATAAACCGTGTTACAAGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.002

98.768

0.583
  pilB Acinetobacter baumannii D1279779

57.876

99.472

0.576
  pilB Legionella pneumophila strain ERS1305867

55.83

99.648

0.556
  pilB Vibrio cholerae strain A1552

51.872

98.768

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50.794

99.824

0.507
  pilB Vibrio campbellii strain DS40M4

50.446

98.768

0.498
  pilF Neisseria gonorrhoeae MS11

47.08

99.472

0.468
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.628

97.535

0.377
  pilF Thermus thermophilus HB27

37.143

98.592

0.366
  pilB/pilB1 Synechocystis sp. PCC 6803

39.619

92.43

0.366