Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K0H80_RS17540 Genome accession   NZ_CP080416
Coordinates   4044817..4046526 (+) Length   569 a.a.
NCBI ID   WP_220072429.1    Uniprot ID   -
Organism   Shewanella aegiceratis strain FJAT-53532     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4039817..4051526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H80_RS17520 (K0H80_17520) ampD 4039928..4040473 (-) 546 WP_220072425.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K0H80_RS17525 (K0H80_17525) nadC 4040759..4041640 (+) 882 WP_220072426.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0H80_RS19780 (K0H80_17530) - 4042575..4042928 (+) 354 WP_220072427.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K0H80_RS17535 (K0H80_17535) - 4043105..4044475 (-) 1371 WP_220072428.1 O-antigen ligase family protein -
  K0H80_RS17540 (K0H80_17540) pilB 4044817..4046526 (+) 1710 WP_220072429.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0H80_RS17545 (K0H80_17545) pilC 4046563..4047831 (+) 1269 WP_011867235.1 type II secretion system F family protein Machinery gene
  K0H80_RS17550 (K0H80_17550) pilD 4047869..4048780 (+) 912 WP_220072430.1 A24 family peptidase Machinery gene
  K0H80_RS17555 (K0H80_17555) coaE 4048800..4049405 (+) 606 WP_220072431.1 dephospho-CoA kinase -
  K0H80_RS17560 (K0H80_17560) zapD 4049414..4050148 (+) 735 WP_144202495.1 cell division protein ZapD -
  K0H80_RS17565 (K0H80_17565) yacG 4050197..4050409 (+) 213 WP_041407493.1 DNA gyrase inhibitor YacG -
  K0H80_RS17570 (K0H80_17570) mutT 4050410..4050799 (+) 390 WP_011867240.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62980.20 Da        Isoelectric Point: 5.1148

>NTDB_id=592722 K0H80_RS17540 WP_220072429.1 4044817..4046526(+) (pilB) [Shewanella aegiceratis strain FJAT-53532]
MPSTGLHLGLSTLFIRKGLLSEEQLSEAVALSRKNKQPLVSTLIATKVLSAREVAELCYEEYGTPLLDLNEFDISSIPEA
FLNKKLIEKHKCLPLFKRGNRLYIGTADPTNIAALEDFQFSAGLHAEAILVEDDKLTIALEKVLEEDISALDLDGIDEDS
LSGIEITDTDKRQEEQQGDASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSGRISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYENDQRKLYEEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECRQPEEVPEHELLKLGF
TQAQIDEGFTVYKPVGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATQAKEQGMRDLRESGLRKVIAGVTSI
AEINRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=592722 K0H80_RS17540 WP_220072429.1 4044817..4046526(+) (pilB) [Shewanella aegiceratis strain FJAT-53532]
ATGCCTTCAACCGGTCTACATTTGGGATTATCGACCCTTTTTATTCGCAAAGGACTACTTAGCGAAGAGCAATTGTCAGA
AGCCGTAGCTCTATCCCGTAAAAACAAGCAACCTCTAGTCAGTACTTTAATTGCTACCAAAGTGCTTTCAGCTAGGGAAG
TGGCAGAGCTATGCTATGAAGAGTATGGTACGCCTTTGTTGGACTTAAATGAATTTGATATCAGCAGCATTCCGGAAGCA
TTCCTAAATAAAAAGCTAATTGAAAAACACAAGTGTTTACCACTATTCAAACGTGGAAATCGCCTTTATATTGGAACAGC
AGATCCTACAAACATCGCGGCTCTAGAGGACTTTCAATTCAGTGCCGGATTGCATGCGGAAGCAATTCTTGTAGAGGATG
ATAAGCTTACAATTGCATTAGAAAAAGTTCTTGAAGAAGATATCTCTGCGCTCGACCTAGACGGTATAGATGAAGACTCT
CTATCTGGCATCGAGATTACCGATACAGACAAGCGCCAGGAAGAACAACAGGGCGACGCAAGTGACGATGCTCCTATCGT
CATATATATAAACAAGATACTCACCGACGCCATACGTAAAGGCGCGTCAGACTTACACTTCGAACCTTATGAAAAACGCT
ATCGTATTCGATTTCGCATCGATGGTATTTTACATGAAGTATCAGAACCTCCTGTTAACTTATCAGGACGAATTTCAGCG
CGTCTAAAAGTAATGTCAAAACTCGATATCGCCGAACGTCGAGTTCCTCAAGATGGCCGAATTAAGATGAAGCTGTCTCG
CACTAAATCTATCGATTTTCGTGTCAGCACCTTGCCGACAATTTGGGGCGAAAAGATCGTGATGCGTATCTTAGACTCCT
CTTCTGCCCAACTCGGTATTGAAAAGCTCGGCTACGAAAATGATCAACGCAAACTCTATGAAGAAATGTTGGCAAAACCT
CAAGGCATGATCTTGGTAACCGGCCCTACAGGCTCAGGTAAAACGGTATCCCTTTATACCGGCCTTAATATTCTCAATAC
CGAAGAGCGCAACATTTCAACCGCTGAAGACCCAGTCGAAATTAACTTAGAAGGCGTCAATCAGGTTCATATTAACTTGA
AGGCTGGACTCACCTTCGCCTCGGCGCTGCGCTCTTTCTTACGTCAAGATCCCGATGTGGTCATGGTGGGTGAGATACGT
GACTTGGAAACCGCCGAAATTGCCATTAAAGCAGCACAAACCGGTCACTTGGTACTTTCGACCCTTCATACCAACTCGGC
TGCCGAAACGCTAACTCGTCTGATAAATATGGGCGTCCCAGGTTACAACATTGCCAGCTCAGTTAACCTAATTATCGCCC
AACGTCTCGCCAGAAGGCTTTGTACCGAATGCCGTCAACCGGAAGAAGTCCCCGAACACGAACTACTGAAACTCGGTTTT
ACACAAGCACAAATTGATGAAGGCTTTACCGTCTATAAGCCAGTTGGCTGTGACCTTTGCTCTGGCGGTTATAAGGGACG
TGTCGGTATTTATGAAGTGATGAAGATGTCAGATGAGATTGCCCGTACTATTATGGAAGGAGGAAATTCTTTGCAAATTG
CCACCCAGGCAAAAGAGCAAGGAATGCGAGATCTCAGAGAATCTGGGCTACGAAAGGTTATTGCCGGCGTAACAAGTATT
GCCGAAATAAATCGTGTAACTAGCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.111

98.594

0.573
  pilB Acinetobacter baumannii D1279779

57.726

98.946

0.571
  pilB Legionella pneumophila strain ERS1305867

55.477

99.473

0.552
  pilB Vibrio campbellii strain DS40M4

51.59

99.473

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

50.441

99.649

0.503
  pilB Vibrio cholerae strain A1552

54.064

92.97

0.503
  pilF Neisseria gonorrhoeae MS11

47.173

99.473

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

37.521

100

0.399
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.503

98.594

0.38
  pilF Thermus thermophilus HB27

37.699

99.297

0.374