Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K0H61_RS01590 Genome accession   NZ_CP080413
Coordinates   344593..346302 (-) Length   569 a.a.
NCBI ID   WP_220051031.1    Uniprot ID   -
Organism   Shewanella acanthi strain FJAT-51860     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 339593..351302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K0H61_RS01560 (K0H61_01560) mutT 339908..340303 (-) 396 WP_220051026.1 8-oxo-dGTP diphosphatase MutT -
  K0H61_RS01565 (K0H61_01565) yacG 340405..340614 (-) 210 WP_011621218.1 DNA gyrase inhibitor YacG -
  K0H61_RS01570 (K0H61_01570) zapD 340736..341470 (-) 735 WP_220051027.1 cell division protein ZapD -
  K0H61_RS01575 (K0H61_01575) coaE 341463..342080 (-) 618 WP_220051028.1 dephospho-CoA kinase -
  K0H61_RS01580 (K0H61_01580) pilD 342139..343074 (-) 936 WP_220051029.1 A24 family peptidase Machinery gene
  K0H61_RS01585 (K0H61_01585) pilC 343246..344511 (-) 1266 WP_220051030.1 type II secretion system F family protein Machinery gene
  K0H61_RS01590 (K0H61_01590) pilB 344593..346302 (-) 1710 WP_220051031.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K0H61_RS01595 (K0H61_01595) - 346360..346851 (-) 492 WP_286670284.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  K0H61_RS01600 (K0H61_01600) nadC 347958..348839 (-) 882 WP_220051032.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K0H61_RS01605 (K0H61_01605) ampD 349292..349834 (+) 543 WP_220051033.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K0H61_RS01610 (K0H61_01610) ampE 349990..350841 (+) 852 WP_220051034.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62763.02 Da        Isoelectric Point: 5.7179

>NTDB_id=592640 K0H61_RS01590 WP_220051031.1 344593..346302(-) (pilB) [Shewanella acanthi strain FJAT-51860]
MPTSGLHLGLSTLFIRKGLLNEEQMATAIAKSRQSKQALVSTLVQTQLISSRSIAELCYEEYGTPLLDLAEFDIGAIPDE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEEFQFSAGLHAEAILVEEDKLAKALEKVLEEDLSSLDLGGMDEAA
LAGIEVTDTDKRPEDNADGSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRLSAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCTECKQEEHIPEHELLRLGFT
QDQISQGFKTYKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIADQAKSQGMRDLRESGLLKVVQGVTSIA
EVNRVTSFG

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=592640 K0H61_RS01590 WP_220051031.1 344593..346302(-) (pilB) [Shewanella acanthi strain FJAT-51860]
ATGCCTACATCCGGTCTTCATTTAGGTTTATCAACTCTCTTTATTCGTAAGGGTCTGCTTAATGAAGAGCAGATGGCCAC
TGCTATTGCCAAATCGAGGCAATCAAAGCAAGCATTAGTAAGTACTCTAGTACAAACCCAGCTGATTTCATCCCGTAGCA
TTGCCGAGCTTTGCTATGAAGAATATGGCACCCCGCTACTGGATTTGGCCGAATTTGATATTGGCGCCATCCCCGATGAG
TTCCTGAATAAAAAACTAATAGAGAAACACCGCTGTTTACCATTATTTAAGCGCGGTAACCGCCTCTATATTGCCACATC
CGATCCCACCAATATTGCCGCCCTCGAAGAATTTCAATTTAGTGCAGGTTTGCACGCTGAAGCGATTTTGGTGGAAGAGG
ATAAGCTAGCCAAAGCCTTAGAAAAGGTACTCGAAGAAGATTTATCCAGTCTCGATTTAGGTGGAATGGATGAGGCGGCA
CTTGCGGGAATAGAAGTTACCGATACCGACAAACGGCCAGAAGATAATGCCGATGGCAGCGATGATGCACCCATTGTTAT
CTATATCAATAAAATCCTAACAGATGCCATCCGTAAGGGCGCATCGGATTTGCATTTCGAGCCCTACGAGAAGCGCTACC
GTATTCGTTTTCGCATCGACGGTATTTTGCATGAAGTGTCTGAACCACCTATTAGCTTATCAGGACGCTTATCAGCGCGC
TTAAAGGTGATGTCGAAACTCGATATTGCTGAGCGCCGAGTCCCCCAAGATGGCCGAATTAAGATGAAGCTTTCCCGCAC
TAAGTCCATCGACTTTCGGGTAAGTACACTTCCGACCCTCTGGGGTGAAAAGATTGTAATGCGGATTTTGGATTCCTCCT
CCGCACAATTGGGCATCGAAAAACTGGGCTATGAGCCGGACCAAGAAAAGCTTTACCTTGAGATGCTAGCCAAACCCCAA
GGGATGATCTTAGTCACTGGCCCTACGGGTTCGGGTAAAACAGTATCTCTATATACTGGTTTAAATATTTTAAATACCAG
CGAACGTAATATCTCCACCGCTGAGGACCCGGTCGAAATTAACCTTGAAGGGGTTAACCAAGTTCATATTAACTTAAAGG
CGGGCTTAACCTTCGCCTCTGCGCTACGCTCATTTTTACGTCAAGACCCCGATGTAGTGATGGTTGGGGAAATTCGTGAC
CTTGAAACCGCAGAGATTGCCATTAAGGCGGCACAAACGGGTCACTTGGTGTTATCGACGCTGCATACCAACTCCGCTGC
CGAAACCCTCACCCGTTTAATCAACATGGGTGTACCTGGTTATAATATCGCAAGCTCCGTAAACCTGATCATTGCCCAGC
GTCTTGCACGGCGTTTATGTACTGAGTGTAAGCAAGAAGAGCATATTCCCGAGCATGAACTGCTTCGTTTAGGCTTTACC
CAAGATCAAATTTCCCAAGGCTTTAAAACCTATAAACCCGTAGGTTGCGAGCACTGCTCCGGCGGCTATAAGGGCCGAGT
CGGTATTTACGAAGTGATGAAAATGTCCGATGAAATTGCCCGAACTATTATGGAAGGCGGCAATTCACTGCAGATTGCCG
ATCAAGCCAAATCACAGGGAATGCGCGATCTGCGTGAATCTGGCTTATTAAAAGTAGTTCAGGGCGTAACCAGTATCGCC
GAAGTGAATCGCGTCACTAGTTTTGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.715

98.594

0.589
  pilB Acinetobacter baumannii D1279779

58.377

99.649

0.582
  pilB Legionella pneumophila strain ERS1305867

56.228

98.77

0.555
  pilB Vibrio cholerae strain A1552

52.899

97.012

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.15

99.297

0.508
  pilF Neisseria gonorrhoeae MS11

49.825

100

0.499
  pilB Vibrio campbellii strain DS40M4

50.356

98.77

0.497
  pilF Thermus thermophilus HB27

38.448

99.649

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.309

97.715

0.374
  pilB/pilB1 Synechocystis sp. PCC 6803

41.968

87.522

0.367