Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCA10_RS04775 Genome accession   NC_021499
Coordinates   1026433..1028139 (-) Length   568 a.a.
NCBI ID   WP_016490897.1    Uniprot ID   S6AFQ2
Organism   Metapseudomonas resinovorans NBRC 106553     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1021433..1033139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCA10_RS04735 (PCA10_09550) - 1021608..1022030 (+) 423 WP_016490889.1 GNAT family N-acetyltransferase -
  PCA10_RS04740 (PCA10_09560) - 1022035..1022502 (+) 468 WP_041770136.1 hypothetical protein -
  PCA10_RS04745 (PCA10_09570) - 1022499..1023188 (+) 690 WP_016490891.1 energy-coupling factor ABC transporter permease -
  PCA10_RS04750 (PCA10_09580) - 1023308..1023523 (+) 216 WP_016490892.1 hypothetical protein -
  PCA10_RS04755 (PCA10_09590) yacG 1023533..1023733 (-) 201 WP_016490893.1 DNA gyrase inhibitor YacG -
  PCA10_RS04760 (PCA10_09600) coaE 1023730..1024341 (-) 612 WP_016490894.1 dephospho-CoA kinase -
  PCA10_RS04765 (PCA10_09610) pilD 1024338..1025210 (-) 873 WP_016490895.1 A24 family peptidase Machinery gene
  PCA10_RS04770 (PCA10_09620) pilC 1025213..1026430 (-) 1218 WP_016490896.1 type II secretion system F family protein Machinery gene
  PCA10_RS04775 (PCA10_09630) pilB 1026433..1028139 (-) 1707 WP_016490897.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCA10_RS04780 (PCA10_09640) pilA2 1028411..1028857 (+) 447 WP_016490898.1 pilin Machinery gene
  PCA10_RS04785 (PCA10_09650) - 1028913..1030628 (+) 1716 WP_041770137.1 PglL family O-oligosaccharyltransferase -
  PCA10_RS04795 (PCA10_09660) nadC 1030831..1031679 (-) 849 WP_016490900.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62812.84 Da        Isoelectric Point: 5.5944

>NTDB_id=59196 PCA10_RS04775 WP_016490897.1 1026433..1028139(-) (pilB) [Metapseudomonas resinovorans NBRC 106553]
MNETAPTLSGLAKQLVLTNLLDERSALQAHTQAQRNKIPLITYLVQNKLLKSRDLAEMAAEQFGVAFLDLGSVDKESQPR
DLVSEKLIRQHRALPLWRRGNKLFVAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIEKFFDSATSGMEELGDVDL
DGVDVEAVDDDKKDTPGGVDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERSYRVRFRTDGMLHEVAKPPIQLASRISA
RLKVMAALDISERRKPQDGRIKMKISKTKSIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYLAALKQP
QGMILVTGPTGSGKTVSLYTGINILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSHCKKEHEVPRETLLKEGF
PEEKIGSFKLYSPVGCDNCNQGYKGRVGIYEVVKITPALQQLIMEEGNSIQIAQQARRDGFNDLRTSGLVKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=59196 PCA10_RS04775 WP_016490897.1 1026433..1028139(-) (pilB) [Metapseudomonas resinovorans NBRC 106553]
ATGAACGAGACCGCTCCTACGCTATCCGGCCTGGCCAAACAACTGGTGCTGACCAACCTCCTCGATGAGCGAAGCGCCCT
CCAGGCCCATACCCAGGCACAGCGCAACAAGATCCCGCTGATCACCTACCTGGTACAGAACAAGCTGCTCAAGAGCCGTG
ATCTGGCGGAGATGGCGGCGGAACAGTTCGGCGTGGCCTTTCTCGATCTCGGCAGTGTCGACAAAGAGAGCCAGCCACGT
GATCTGGTCAGCGAAAAGCTGATCCGCCAGCACCGCGCCCTTCCCCTCTGGCGTCGTGGCAACAAGCTCTTCGTTGCCAT
CTCCGACCCCACCAACCATCAGGCCGTGACCGATATCCAGTTCAGCACCGGGCTGAATACCGAAGCCATCCTTGTAGAGG
ACGACAAGCTCGGCGATGCCATCGAGAAGTTCTTCGACTCCGCCACTAGCGGAATGGAAGAACTGGGCGATGTCGACCTG
GATGGCGTGGACGTCGAAGCAGTCGATGATGACAAAAAGGATACTCCTGGCGGCGTTGATGCCGACGACGCCCCCGTGGT
GCGCTTCGTCAACAAGATGCTGCTGGATGCCATTCGCGGCGGCTCCTCTGACCTGCACTTCGAGCCCTACGAGCGCTCCT
ACCGCGTACGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGTCGCATCTCCGCA
CGCCTGAAGGTGATGGCCGCACTGGATATTTCCGAGCGCCGCAAGCCCCAGGACGGCCGGATCAAGATGAAGATTTCCAA
GACGAAATCCATCGACTTCCGGGTCAACACCCTGCCGACCCTCTGGGGCGAGAAGATCGTGATGCGGATCCTCGACCCTA
CCAGCGCGCAGATGGGTATCGATGCACTGGGCTATGAGGAAGAGCAGAAAGAACTCTACCTGGCGGCGCTGAAGCAGCCC
CAGGGCATGATCCTCGTAACCGGCCCTACAGGTTCCGGCAAGACGGTATCCCTCTACACCGGGATCAATATCCTCAATAC
GGTGGATATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTCGAGGGCATCAACCAGGTCAACGTCAATCCCA
AGCAGGGCATGGACTTCGCCCAGGCCCTGCGGGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGC
GACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCATATGGTGCTATCCACCTTGCACACCAACAGCGC
CGCGGAAACCCTGACGCGCCTGCGCAATATGGGTGTGCCAGCCTTCAACATCGCCACGTCGGTCAACCTGATCATCGCCC
AGCGCCTGGCACGCAAATTGTGTAGTCATTGCAAGAAGGAACACGAGGTCCCTAGGGAAACCCTGCTCAAGGAAGGCTTC
CCTGAAGAGAAGATCGGCAGCTTCAAACTCTACTCCCCGGTGGGCTGCGACAATTGCAACCAGGGTTACAAGGGCCGAGT
CGGTATTTATGAAGTGGTTAAAATCACGCCGGCCTTGCAGCAGCTTATTATGGAAGAAGGCAACTCCATCCAGATCGCCC
AGCAGGCGCGCCGTGATGGCTTCAATGACCTGCGAACCTCGGGCCTGGTAAAAGCCATGAATGGCATTACCAGCCTGGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S6AFQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.319

100

0.586
  pilB Acinetobacter baylyi ADP1

58.377

99.824

0.583
  pilB Legionella pneumophila strain ERS1305867

57.597

99.648

0.574
  pilB Vibrio parahaemolyticus RIMD 2210633

50.266

99.12

0.498
  pilB Vibrio campbellii strain DS40M4

49.823

99.296

0.495
  pilF Neisseria gonorrhoeae MS11

49.734

99.12

0.493
  pilB Vibrio cholerae strain A1552

52.481

92.254

0.484
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.134

0.368
  pilF Thermus thermophilus HB27

38.868

93.31

0.363

Multiple sequence alignment