Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MYF74_RS18610 Genome accession   NZ_CP096837
Coordinates   3945565..3946950 (+) Length   461 a.a.
NCBI ID   WP_001025189.1    Uniprot ID   -
Organism   Escherichia coli strain PS10-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3940565..3951950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYF74_RS18585 (MYF74_18585) aroP 3941113..3942486 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  MYF74_RS18590 (MYF74_18590) ampE 3942529..3943383 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  MYF74_RS18595 (MYF74_18595) ampD 3943380..3943931 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MYF74_RS18600 (MYF74_18600) nadC 3944019..3944912 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MYF74_RS18605 (MYF74_18605) pilA 3945115..3945555 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  MYF74_RS18610 (MYF74_18610) pilB 3945565..3946950 (+) 1386 WP_001025189.1 type II secretion system protein GspE Machinery gene
  MYF74_RS18615 (MYF74_18615) hofC 3946940..3948142 (+) 1203 WP_218075111.1 protein transport protein HofC -
  MYF74_RS18620 (MYF74_18620) guaC 3948177..3949220 (-) 1044 WP_001217338.1 GMP reductase -
  MYF74_RS18625 (MYF74_18625) - 3949376..3949420 (-) 45 WP_120795372.1 protein YacM -
  MYF74_RS18630 (MYF74_18630) coaE 3949445..3950065 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  MYF74_RS18635 (MYF74_18635) zapD 3950065..3950808 (+) 744 WP_001194734.1 cell division protein ZapD -
  MYF74_RS18640 (MYF74_18640) yacG 3950818..3951015 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  MYF74_RS18645 (MYF74_18645) mutT 3951337..3951735 (-) 399 WP_000736016.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50634.31 Da        Isoelectric Point: 6.6268

>NTDB_id=590978 MYF74_RS18610 WP_001025189.1 3945565..3946950(+) (pilB) [Escherichia coli strain PS10-3]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQKLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TTETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=590978 MYF74_RS18610 WP_001025189.1 3945565..3946950(+) (pilB) [Escherichia coli strain PS10-3]
ATGAATATTCCACAGCTCACCGCCCTGTGTTTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAAGTGGTTCATGT
TGCGGTGGTCGATGCCCCCTCACATGAATTGCTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGCCAGGCACTGGATGTCAACACGCTGGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CGCAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAAAGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATTCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATTATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCACTGAAACGCTGGTACGTTTACAGCAAATGGGGGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCACATTGTCGCCGCCAGCAAGGGGAGCCCATCCACATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCATTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACTGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

40.385

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Acinetobacter baumannii D1279779

43.909

85.466

0.375
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36