Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MYF74_RS18605 Genome accession   NZ_CP096837
Coordinates   3945115..3945555 (+) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain PS10-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3940115..3950555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYF74_RS18585 (MYF74_18585) aroP 3941113..3942486 (+) 1374 WP_000969912.1 aromatic amino acid transporter AroP -
  MYF74_RS18590 (MYF74_18590) ampE 3942529..3943383 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  MYF74_RS18595 (MYF74_18595) ampD 3943380..3943931 (-) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MYF74_RS18600 (MYF74_18600) nadC 3944019..3944912 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MYF74_RS18605 (MYF74_18605) pilA 3945115..3945555 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  MYF74_RS18610 (MYF74_18610) pilB 3945565..3946950 (+) 1386 WP_001025189.1 type II secretion system protein GspE Machinery gene
  MYF74_RS18615 (MYF74_18615) hofC 3946940..3948142 (+) 1203 WP_218075111.1 protein transport protein HofC -
  MYF74_RS18620 (MYF74_18620) guaC 3948177..3949220 (-) 1044 WP_001217338.1 GMP reductase -
  MYF74_RS18625 (MYF74_18625) - 3949376..3949420 (-) 45 WP_120795372.1 protein YacM -
  MYF74_RS18630 (MYF74_18630) coaE 3949445..3950065 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=590977 MYF74_RS18605 WP_000360901.1 3945115..3945555(+) (pilA) [Escherichia coli strain PS10-3]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=590977 MYF74_RS18605 WP_000360901.1 3945115..3945555(+) (pilA) [Escherichia coli strain PS10-3]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363