Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KW568_RS27170 Genome accession   NZ_CP079827
Coordinates   5923788..5925050 (-) Length   420 a.a.
NCBI ID   WP_080634927.1    Uniprot ID   -
Organism   Pseudomonas sp. HD6515     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5918788..5930050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW568_RS27155 (KW568_26925) - 5920454..5922055 (-) 1602 WP_003249151.1 SPOR domain-containing protein -
  KW568_RS27160 (KW568_26930) aroB 5922071..5923168 (-) 1098 WP_004575493.1 3-dehydroquinate synthase -
  KW568_RS27165 (KW568_26935) aroK 5923265..5923783 (-) 519 WP_003249147.1 shikimate kinase AroK -
  KW568_RS27170 (KW568_26940) pilQ 5923788..5925050 (-) 1263 WP_080634927.1 type IV pilus secretin PilQ Machinery gene
  KW568_RS27175 (KW568_26945) - 5924974..5925975 (-) 1002 WP_004575491.1 pilus assembly protein PilP -
  KW568_RS27180 (KW568_26950) - 5925987..5926523 (-) 537 WP_004575490.1 PilN domain-containing protein -
  KW568_RS27185 (KW568_26955) pilM 5926520..5927401 (-) 882 WP_004575489.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45670.35 Da        Isoelectric Point: 4.9839

>NTDB_id=589828 KW568_RS27170 WP_080634927.1 5923788..5925050(-) (pilQ) [Pseudomonas sp. HD6515]
MKTWMKYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQVLDAQLQPLRRELLTIHHAKAADLAQLLLASLEGGGISTGSLSVDERT
NTLVVYLPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=589828 KW568_RS27170 WP_080634927.1 5923788..5925050(-) (pilQ) [Pseudomonas sp. HD6515]
ATGAAAACGTGGATGAAATACCTGCTGGCAACCCTGCTGTCGATCCCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTAGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCCGGCGTCAACCTGGTCG
CCAGTGATGCGGTACAAGGCAGCGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCACTTGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGCCAAGTGCTGGACGCGCAGCTGCAGCCATTGCGTCGGGAACTGCTGACCATTCACCACGCCAAGGCCG
CCGATTTGGCGCAGTTGCTGCTGGCAAGTCTGGAGGGTGGCGGCATCTCCACGGGTAGCCTGAGTGTCGATGAGCGCACC
AATACGCTGGTGGTGTACCTGCCCGCTGACCGCCTGGCCGAGTTACGCCAGCTGGTGGCGCAGTTGGATGTGCCGGTGCG
TCAGGTAGCGATCGAAGCGCGTATCGTCGAAGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTTGTCGACCTCGGTCTGGAGCGAGCCGGCAGCAGTATCGGCCTG
GGTTTGTTGCGCGGTGACGTGTTGCTGGATCTGGAACTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCTGACAAGGAAACGGCGAGGATCCTCAAGGGTACCGAGGTGCCTTACCAGGAAACCAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACTCCGGACAACAAGGTG
ATCATGACCGTCCGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTTCGTGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(126-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.633

100

0.619
  comQ Acinetobacter baylyi ADP1

42.889

100

0.46
  pilQ Acinetobacter baumannii strain A118

40.998

100

0.45
  pilQ Acinetobacter baumannii D1279779

40.998

100

0.45
  pilQ Legionella pneumophila strain Lp02

43.269

99.048

0.429
  pilQ Legionella pneumophila strain ERS1305867

43.269

99.048

0.429
  pilQ Vibrio campbellii strain DS40M4

41.014

100

0.424
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

98.81

0.419
  pilQ Vibrio cholerae strain A1552

42.41

98.81

0.419