Detailed information NTG0589536

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EGT72_RS14335
• Genome accession: NZ_CP079792
• Coordinates: 2888755..2889813 (-)
• Length: 352 a.a.
• NCBI ID: WP_004978085.1
• Uniprot ID: N9D0Y2
• Organism: Acinetobacter johnsonii strain AJ_082
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2883755..2894813

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGT72_RS18465	-	2884184..2884666 (-)	483	WP_252715852.1	shikimate kinase	-
EGT72_RS14315 (EGT72_014315)	pilQ	2884755..2886833 (-)	2079	WP_125280898.1	type IV pilus secretin PilQ	Machinery gene
EGT72_RS14320 (EGT72_014320)	pilP	2886853..2887380 (-)	528	WP_004691496.1	pilus assembly protein PilP	Machinery gene
EGT72_RS14325 (EGT72_014325)	pilO	2887380..2888102 (-)	723	WP_004691497.1	type 4a pilus biogenesis protein PilO	Machinery gene
EGT72_RS14330 (EGT72_014330)	pilN	2888099..2888755 (-)	657	WP_004691498.1	PilN domain-containing protein	Machinery gene
EGT72_RS14335 (EGT72_014335)	pilM	2888755..2889813 (-)	1059	WP_004978085.1	pilus assembly protein PilM	Machinery gene
EGT72_RS14340 (EGT72_014340)	ponA	2889979..2892507 (+)	2529	WP_125280897.1	penicillin-binding protein PBP1a	-
EGT72_RS14345 (EGT72_014345)	-	2892539..2893345 (-)	807	WP_125280896.1	putative RNA methyltransferase	-
EGT72_RS14350 (EGT72_014350)	mraY	2893415..2894533 (-)	1119	WP_125280895.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38937.50 Da
• Isoelectric Point: 4.4181

>NTDB_id=589536 EGT72_RS14335 WP_004978085.1 2888755..2889813(-) (pilM) [Acinetobacter johnsonii strain AJ_082]
MLRLYRKPSKGLIGVDISSTSVKVLELSVKNNRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAVNLANVQSTDVAL
AVPTSMVITKIIEMDADMNDDEREIQIRDDAEQYIPFPLDEASLDFEVLPDRLANPNRVNVLLVATRIENVEARAEALEL
GGLTPKIADVESFAIENAFKVFSDTLPMGVNTVGILDIGHTMTTLSVMQNNKVIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDIEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDIKKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=589536 EGT72_RS14335 WP_004978085.1 2888755..2889813(-) (pilM) [Acinetobacter johnsonii strain AJ_082]
GTGCTCAGGTTATATCGTAAGCCAAGTAAGGGGTTAATAGGAGTAGATATTAGTTCGACTTCTGTTAAGGTATTGGAACT
TTCTGTTAAAAACAATCGGTATTGGGTGGAGAGCTATGCACTAGTCCCATTACCAGAAGGAAGTGTGGTCGAAAAAAATA
TATTGAATCCAGAAGCAGTTGGTGATGCACTTGAGCGTGCGGTAAATTTGGCCAATGTTCAATCGACAGACGTAGCACTG
GCAGTTCCGACGTCCATGGTGATTACTAAAATCATCGAAATGGATGCCGATATGAACGATGACGAACGTGAAATTCAGAT
TCGTGATGATGCTGAACAATATATTCCATTCCCATTAGACGAAGCGAGTTTGGACTTTGAAGTCCTTCCAGACCGCTTAG
CCAATCCCAATCGTGTCAATGTACTCCTCGTTGCAACCCGCATTGAAAATGTAGAGGCGCGTGCCGAAGCATTGGAGTTG
GGTGGTTTAACGCCTAAGATTGCCGATGTTGAAAGTTTTGCCATTGAAAATGCATTCAAAGTCTTTTCAGATACGCTTCC
AATGGGTGTAAACACCGTTGGGATTCTGGACATTGGTCACACCATGACGACCTTATCTGTCATGCAGAATAATAAAGTCA
TTTATACCCGTGAACAGGTGTTCGGTGGTAAACAGCTGACTCAAGAAATTCAAAACCGTTATGGTTTGTCTTTTGAAGAA
GCTGGACGTGCCAAAAAAAGCCGTACCTTGCCTGACGACTATGATATTGAAGTACTTGAACCTTTTCTTGAGGCTGTGGT
GCAACAAGCAGCACGTTCTTTGCAGTTCTTTTTTTCGTCCTCTCAATTTAATGAGATCGACCATATTTTATTGGCAGGGG
GGAATGCCAATATTCCAGGACTGGCGAAGTTATTACAACAAAAATTAGGTTATCGGGTCACCATTGCCAACCCTTTTTTA
CAGATGGGCTTTTCTCCACAAATCGACATTAAAAAAATTGAAAATGATGCTTCATCACTCATGGTGGCATGTGGATTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9D0Y2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	85.227	100	0.852
comM	Acinetobacter nosocomialis M2	85.227	100	0.852
comM	Acinetobacter baylyi ADP1	80.398	100	0.804
pilM	Legionella pneumophila strain ERS1305867	40.96	100	0.412