Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EGT72_RS14325 Genome accession   NZ_CP079792
Coordinates   2887380..2888102 (-) Length   240 a.a.
NCBI ID   WP_004691497.1    Uniprot ID   A0A380U8X3
Organism   Acinetobacter johnsonii strain AJ_082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2882380..2893102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT72_RS14310 (EGT72_014310) aroB 2883068..2884153 (-) 1086 WP_125280900.1 3-dehydroquinate synthase -
  EGT72_RS18465 - 2884184..2884666 (-) 483 WP_252715852.1 shikimate kinase -
  EGT72_RS14315 (EGT72_014315) pilQ 2884755..2886833 (-) 2079 WP_125280898.1 type IV pilus secretin PilQ Machinery gene
  EGT72_RS14320 (EGT72_014320) pilP 2886853..2887380 (-) 528 WP_004691496.1 pilus assembly protein PilP Machinery gene
  EGT72_RS14325 (EGT72_014325) pilO 2887380..2888102 (-) 723 WP_004691497.1 type 4a pilus biogenesis protein PilO Machinery gene
  EGT72_RS14330 (EGT72_014330) pilN 2888099..2888755 (-) 657 WP_004691498.1 PilN domain-containing protein Machinery gene
  EGT72_RS14335 (EGT72_014335) pilM 2888755..2889813 (-) 1059 WP_004978085.1 pilus assembly protein PilM Machinery gene
  EGT72_RS14340 (EGT72_014340) ponA 2889979..2892507 (+) 2529 WP_125280897.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 240 a.a.        Molecular weight: 27004.43 Da        Isoelectric Point: 4.9937

>NTDB_id=589534 EGT72_RS14325 WP_004691497.1 2887380..2888102(-) (pilO) [Acinetobacter johnsonii strain AJ_082]
MSRDEFEELTSENHAVTKKKMTVDKFFQQFNTLDPNNYGSWPFSVKLTCWIFIILVIGALGYFVVIKPKLDAISNAHAQE
QNLLNEFREKDSKLRNLQQYQAQLQEMEANFNQQLEQLPKETEIPGLVEDINVSGVNSGLKFKNIRLEPEIRQEFFIEQP
ISIEATGDYHSFGSFVSGIAALPRIVTLHDFDISASSNNEKKTDIPQISYSVKAKTYRYVGATAAASVENTAKADAGGAQ

Nucleotide


Download         Length: 723 bp        

>NTDB_id=589534 EGT72_RS14325 WP_004691497.1 2887380..2888102(-) (pilO) [Acinetobacter johnsonii strain AJ_082]
ATGAGTCGCGATGAATTTGAAGAATTAACATCAGAAAACCATGCTGTAACTAAGAAGAAAATGACGGTTGATAAGTTTTT
TCAACAGTTCAATACTTTGGATCCCAATAATTACGGCAGTTGGCCCTTTTCAGTCAAATTGACTTGTTGGATCTTTATCA
TTCTAGTGATTGGGGCATTGGGTTATTTTGTCGTGATTAAGCCGAAGCTTGATGCGATTTCAAATGCACATGCACAAGAA
CAAAATTTACTGAATGAGTTTCGTGAAAAAGATTCAAAATTACGTAATTTGCAGCAATATCAAGCGCAATTGCAAGAAAT
GGAAGCCAATTTTAATCAGCAGTTAGAACAGCTGCCGAAAGAAACTGAAATTCCTGGTTTGGTTGAAGATATCAACGTGA
GCGGCGTAAATTCTGGATTAAAATTTAAAAATATTCGTTTAGAGCCAGAAATTCGCCAAGAGTTCTTTATCGAACAGCCG
ATTTCCATTGAAGCGACGGGTGATTATCATTCTTTTGGTTCATTTGTCAGTGGAATTGCAGCTTTACCGCGTATTGTGAC
CTTGCATGACTTCGATATCTCAGCATCAAGTAATAATGAGAAAAAGACGGATATTCCGCAAATTTCATACAGTGTAAAGG
CTAAAACTTACCGCTATGTCGGGGCGACAGCTGCTGCAAGTGTGGAAAATACAGCTAAAGCAGATGCAGGAGGTGCTCAG
TAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(74-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A380U8X3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

72.385

99.583

0.721
  comO Acinetobacter baylyi ADP1

67.755

100

0.692