Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EGT72_RS01605 Genome accession   NZ_CP079792
Coordinates   329614..331326 (-) Length   570 a.a.
NCBI ID   WP_125280996.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ_082     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 324614..336326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT72_RS01575 (EGT72_001575) - 324712..325035 (+) 324 WP_125280995.1 pyrimidine/purine nucleoside phosphorylase -
  EGT72_RS01580 (EGT72_001580) rlmB 325178..325927 (+) 750 WP_004978746.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  EGT72_RS01585 (EGT72_001585) - 325959..326876 (+) 918 WP_171428537.1 DMT family transporter -
  EGT72_RS01590 (EGT72_001590) coaE 326891..327490 (-) 600 WP_125273378.1 dephospho-CoA kinase -
  EGT72_RS01595 (EGT72_001595) pilD 327497..328357 (-) 861 WP_125273377.1 A24 family peptidase Machinery gene
  EGT72_RS01600 (EGT72_001600) pilC 328357..329583 (-) 1227 WP_004692713.1 type II secretion system F family protein Machinery gene
  EGT72_RS01605 (EGT72_001605) pilB 329614..331326 (-) 1713 WP_125280996.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGT72_RS01610 (EGT72_001610) tpiA 331606..332397 (+) 792 WP_010325769.1 triose-phosphate isomerase -
  EGT72_RS01615 (EGT72_001615) secG 332411..332740 (+) 330 WP_125280997.1 preprotein translocase subunit SecG -
  EGT72_RS01645 (EGT72_001645) rimP 333690..334214 (+) 525 WP_004692708.1 ribosome maturation factor RimP -
  EGT72_RS01650 (EGT72_001650) nusA 334254..335738 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63612.11 Da        Isoelectric Point: 5.0100

>NTDB_id=589507 EGT72_RS01605 WP_125280996.1 329614..331326(-) (pilB) [Acinetobacter johnsonii strain AJ_082]
MSVSNGLPKFTGFIRRLVEDGRMTAENMQQAMLSAKKAQQDIVPYLIENYKLSPQMIAETISLEFGEPVFDLSVYDSAQI
LRDVVDIKLITKHRILPIFRRGNILYLATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKFIEQNFAQANSFEFADDDI
DLDLEQDFEKPNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGVNQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCAECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=589507 EGT72_RS01605 WP_125280996.1 329614..331326(-) (pilB) [Acinetobacter johnsonii strain AJ_082]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCATGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTAATTGAAAATTATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGTGAACCAGTTTTTGACCTAAGTGTCTATGACTCAGCACAAATT
CTACGAGATGTTGTTGATATTAAGCTCATCACTAAACATCGTATTCTACCTATCTTCCGCCGCGGTAATATTCTTTATCT
CGCAACCAGCAATCCGACCAATATGGATGCCATGGATGCAATTCGCTTCAACTCTAAAATGAACATTGAAGCAATTATTG
TTGAACACCCTCAACTGGAAAAATTTATTGAGCAAAATTTTGCACAAGCAAATAGTTTCGAATTTGCTGATGATGATATT
GATTTAGATCTAGAACAAGATTTTGAAAAGCCGAATGATGAGGAGGATGACTCCCCGCAAGGCGATGAAGCGCCAATTGT
AAAATACATCAATAAACTATTGATTGATGCGATTCGTATGGGCGCTTCAGACTTACACTTTGAGCCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCACCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGAATTAAATTAAAGCTGTCGAA
AAGCAAAGCCATCGACTTCCGTGTCAACTCACTTCCAACCTTATTTGGCGAAAAGCTGGTTCTACGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGAGCAAAAAGAGCTGTTTATGCAGGCTTTAGACAAACCA
CAAGGTATGCTGCTAATTACTGGCCCGACGGGTTCAGGGAAAACGGTGTCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCGGAAGATCCCGTTGAGATTAACTTAGAAGGGGTTAATCAAGTCAACGTGAACAATA
AAGTTGGACTGACCTTTTCTGCAGCACTAAAGTCCTTCTTACGTCAAGATCCTGATATTGTGATGGTGGGTGAGATTCGT
GACCTTGAAACGGCTGAAATCGCAATTAAAGCTGCACAGACAGGTCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACCCTGACACGCTTACGTAATATGGGGGTTCCCTCTTTTAACATTGCGACATCGGTCAATTTGGTGATTGCAC
AGCGTTTGGCACGCCGCTTATGCAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGTTTGCTCGAAATGGGCTTT
ACTTCCACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGGTGTGCTGAGTGCCGTGAAGGTTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACGCCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.877

100

0.809
  pilB Acinetobacter baylyi ADP1

77.193

100

0.772
  pilB Legionella pneumophila strain ERS1305867

53.358

94.035

0.502
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

49.286

98.246

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

50.29

90.702

0.456
  pilB Vibrio campbellii strain DS40M4

49.807

90.877

0.453
  pilF Thermus thermophilus HB27

36.538

100

0.367
  pilB/pilB1 Synechocystis sp. PCC 6803

33.659

100

0.363