Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KYK33_RS02590 Genome accession   NZ_CP079742
Coordinates   554404..556110 (-) Length   568 a.a.
NCBI ID   WP_224431931.1    Uniprot ID   -
Organism   Aeromonas rivuli strain 20-VB00005     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 549404..561110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KYK33_RS02560 (KYK33_02555) mutT 549960..550370 (+) 411 WP_224431928.1 8-oxo-dGTP diphosphatase MutT -
  KYK33_RS02565 (KYK33_02560) yacG 550446..550640 (-) 195 WP_042041799.1 DNA gyrase inhibitor YacG -
  KYK33_RS02570 (KYK33_02565) zapD 550702..551424 (-) 723 WP_042041798.1 cell division protein ZapD -
  KYK33_RS02575 (KYK33_02570) coaE 551462..552076 (-) 615 WP_042041797.1 dephospho-CoA kinase -
  KYK33_RS02580 (KYK33_02575) pilD 552143..553015 (-) 873 WP_224431929.1 A24 family peptidase Machinery gene
  KYK33_RS02585 (KYK33_02580) pilC 553100..554341 (-) 1242 WP_224431930.1 type II secretion system F family protein Machinery gene
  KYK33_RS02590 (KYK33_02585) pilB 554404..556110 (-) 1707 WP_224431931.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  KYK33_RS02595 (KYK33_02590) - 556114..556530 (-) 417 WP_224431932.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KYK33_RS02600 (KYK33_02595) nadC 556955..557824 (-) 870 WP_224431933.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KYK33_RS02605 (KYK33_02600) - 557830..558306 (-) 477 WP_042041791.1 TIGR02281 family clan AA aspartic protease -
  KYK33_RS02610 (KYK33_02605) ampD 558418..558993 (+) 576 WP_224432928.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KYK33_RS02615 (KYK33_02610) pdhR 559267..560031 (+) 765 WP_224431934.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62437.64 Da        Isoelectric Point: 5.5250

>NTDB_id=589103 KYK33_RS02590 WP_224431931.1 554404..556110(-) (pilB) [Aeromonas rivuli strain 20-VB00005]
MTSSPHSGLALSLAASSLLSESDSQHYLSQARAQRKPFVTYLIENDILASKTLAEFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIDKHHVLPIYTQGHTLYIAMSDPTNVAALEDFGFSFGLHTEALLVEEDKLSQAIGKLLESEHDALGMDDIDEAEI
SALEVADDSSRLDESVNTADDDAPIVKYINKILMDAIRQGASDLHFEPYEFKYRIRFRIDGILHEASTPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKLSRNRSMDMRVNTLPTMWGEKVVIRLLDSSAARLNIEQLGFDETQKQQYLTALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTLEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKEPEAIPDSELTALGFS
TQQVTAGVRLFKPKGCKECTGGYKGRVGIYEILLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLS
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=589103 KYK33_RS02590 WP_224431931.1 554404..556110(-) (pilB) [Aeromonas rivuli strain 20-VB00005]
ATGACCTCTAGCCCCCATAGCGGCCTGGCCCTGAGCCTGGCCGCTTCCTCCCTGCTAAGTGAGTCTGACTCACAGCACTA
TCTGAGCCAGGCCCGCGCCCAGCGCAAACCCTTTGTCACCTACCTTATCGAGAACGACATCCTCGCCAGCAAGACGCTGG
CCGAGTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGTTGATCGACAAGCACCATGTGCTGCCCATCTACACCCAGGGCCATACCCTCTATATCGCTATGTCCGA
CCCGACCAATGTGGCGGCCCTGGAAGATTTCGGCTTCAGCTTCGGCCTGCATACCGAGGCCCTGCTGGTCGAAGAGGACA
AACTGAGCCAGGCCATCGGCAAGCTGCTCGAGAGCGAGCATGACGCCCTCGGCATGGACGATATTGATGAGGCCGAGATC
TCGGCACTCGAAGTGGCAGACGACAGCAGTCGCCTTGACGAAAGCGTCAACACCGCCGACGACGATGCCCCCATCGTCAA
ATACATCAACAAGATCTTGATGGACGCCATTCGCCAGGGGGCATCCGACCTGCACTTCGAACCCTATGAGTTCAAGTACC
GCATCCGCTTTCGTATCGATGGCATATTGCACGAAGCCTCCACCCCGCCGGTCAATCTGGCCAACCGCTTCGCCGCCCGC
CTCAAGGTCATGGCCAGACTAGACATTGCCGAACGGCGCCTGCCTCAGGATGGTCGCATCAAGCTCAAGCTGTCCCGTAA
CCGCTCCATGGACATGCGGGTCAATACCCTGCCGACCATGTGGGGCGAGAAGGTCGTCATCCGGTTGCTTGACTCCTCGG
CGGCCCGGCTCAACATCGAGCAACTGGGCTTTGACGAGACGCAGAAACAGCAATACCTGACCGCACTGAGTAAACCACAG
GGCATGATCCTGGTGACAGGACCCACCGGCTCCGGCAAGACGGTTTCTCTCTATACCGGGCTCAACATACTCAATACCCT
TGAGGTCAATATCTCCACCGCCGAAGATCCGGTAGAGATCAACCTGCCCGGGGTCAATCAGGTGCAGGTCAATCCCAAGG
CAGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTTCGCCAGGACCCGGATATCGTCATGGTGGGGGAGATCCGGGAT
CTAGAAACCGCCGAGATAGCCATCAAGGCGGCCCAGACCGGCCACCTGGTACTCTCTACCCTGCACACCAACTCGGCAGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCGTCGGTAACGCTCATCATGGCTCAAC
GGCTCGCCCGCAAGCTGTGCGATCACTGCAAGGAACCGGAAGCTATCCCCGACTCCGAACTGACCGCGCTGGGCTTCAGT
ACTCAGCAAGTGACCGCTGGGGTAAGGCTGTTCAAACCCAAGGGTTGCAAGGAGTGTACCGGGGGTTACAAGGGGCGGGT
CGGCATTTATGAAATACTGTTGATGTCAGAAAATATCGCCAAACTCATCATGCAGGGGGCGAACTCGTTGCAGATTGCCG
CCATCGCCCAGAAGGAGGGGATGCGAACCCTGCGCATATCTGGCCTGGAAAAAGCCCGTCTCGGCGTCACCAGTCTGAGC
GAAATCAATAGGATCACCACCAACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.009

100

0.551
  pilB Legionella pneumophila strain ERS1305867

55.044

99.472

0.548
  pilB Acinetobacter baylyi ADP1

54.049

100

0.54
  pilB Vibrio cholerae strain A1552

53.654

98.768

0.53
  pilB Vibrio parahaemolyticus RIMD 2210633

52.135

98.944

0.516
  pilB Vibrio campbellii strain DS40M4

50.444

99.12

0.5
  pilF Neisseria gonorrhoeae MS11

46.277

99.296

0.46
  pilF Thermus thermophilus HB27

41.288

92.958

0.384
  pilB/pilB1 Synechocystis sp. PCC 6803

39.234

96.479

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361