Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KW115_RS13360 Genome accession   NZ_CP079095
Coordinates   2746417..2748132 (+) Length   571 a.a.
NCBI ID   WP_218806195.1    Uniprot ID   -
Organism   Methylococcus sp. Mc7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2741417..2753132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW115_RS13345 (KW115_13345) - 2741589..2743082 (+) 1494 WP_218806192.1 leucyl aminopeptidase -
  KW115_RS13350 (KW115_13350) - 2743079..2743510 (+) 432 WP_218806193.1 DNA polymerase III subunit chi -
  KW115_RS13355 (KW115_13355) - 2743605..2746367 (+) 2763 WP_218806194.1 valine--tRNA ligase -
  KW115_RS13360 (KW115_13360) pilB 2746417..2748132 (+) 1716 WP_218806195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KW115_RS13365 (KW115_13365) pilC 2748137..2749351 (+) 1215 WP_218806196.1 type II secretion system F family protein Machinery gene
  KW115_RS13370 (KW115_13370) pilD 2749362..2750240 (+) 879 WP_218806197.1 A24 family peptidase Machinery gene
  KW115_RS13375 (KW115_13375) coaE 2750248..2750844 (+) 597 WP_218806198.1 dephospho-CoA kinase -
  KW115_RS13380 (KW115_13380) zapD 2750946..2751713 (+) 768 WP_218806199.1 cell division protein ZapD -
  KW115_RS13385 (KW115_13385) yacG 2751724..2751939 (+) 216 WP_218806200.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63105.82 Da        Isoelectric Point: 5.8943

>NTDB_id=587636 KW115_RS13360 WP_218806195.1 2746417..2748132(+) (pilB) [Methylococcus sp. Mc7]
MAAVSGVQQLSGLAKSLVKAGMLSEADAQAHFEDARRKKTPFVSYLVSNKILDGLQIAQVAHQEFGLPLLDLDAVVFDIP
PTQWVSEKLIRRHHILPLLRRGNHLFVAVSDPTNFLGLDEITFQTRLVTDCILVEENKLSRCIEAVLDAADTSLKQLLEE
DLDSLQITAGDDEGSHAEPEISGIDDAPIVRFVNRLLLDAVRKGASDIHIEPYEKFLRIRFRHDGILHEVANPPAALGIK
ICARIKVMSRMDIAEHRIPQDGRIKMTLSRSRAIDFRVNTCPTLFGEKIVLRILDPGSAQIGVEMLGFEPEQRDNFLRAL
EKPYGMILVTGPTGSGKTVTLYTGLNLLNRVEANISTVEDPVEITVPGINQVNVNYKTGLTFAEALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIASSVLLIMAQRLARRLCPHCKREEKLPPEVLLA
SGFREEDIGNFTLYGPGGCEQCVKGYKGRVGIYQVMPISEEINRIILEGGHVMALTEQAHAEGIADLRESGLKKVRAGIT
SLEEIDRVTRD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=587636 KW115_RS13360 WP_218806195.1 2746417..2748132(+) (pilB) [Methylococcus sp. Mc7]
ATGGCCGCGGTTTCCGGCGTCCAGCAACTGAGCGGCCTGGCGAAAAGCCTGGTCAAGGCCGGGATGCTTTCCGAGGCGGA
TGCCCAGGCACATTTCGAGGATGCGCGCCGGAAGAAGACCCCTTTCGTCAGCTATCTCGTCTCCAACAAGATCCTCGACG
GTCTCCAGATCGCCCAGGTCGCCCACCAGGAGTTCGGGCTGCCGCTGCTCGATCTGGACGCGGTCGTCTTCGATATTCCC
CCGACCCAGTGGGTCAGCGAGAAGCTGATACGGCGCCATCACATCCTGCCCTTGCTGCGGCGTGGCAACCATCTGTTCGT
CGCGGTCTCGGATCCGACCAATTTTCTGGGGCTGGACGAAATCACCTTCCAGACCCGTCTCGTCACCGATTGCATCCTGG
TCGAGGAGAACAAACTCTCCCGCTGCATCGAAGCGGTCCTGGACGCGGCCGACACCAGCCTCAAGCAACTGCTCGAGGAG
GACCTGGACAGTCTCCAGATCACCGCCGGGGACGACGAAGGCTCGCATGCCGAACCGGAAATTTCCGGCATCGACGATGC
GCCGATCGTGCGTTTCGTCAACCGGCTCCTGCTGGACGCGGTGCGAAAAGGGGCGTCGGACATCCATATCGAGCCCTACG
AAAAATTCCTGCGCATCCGGTTCCGGCACGACGGCATCCTGCACGAGGTGGCGAACCCGCCGGCGGCGCTGGGAATCAAG
ATCTGCGCCCGCATCAAGGTGATGTCGCGCATGGACATCGCGGAACACCGGATACCCCAGGACGGCCGCATCAAGATGAC
GTTGTCGCGTTCCCGCGCCATCGATTTCCGTGTCAATACCTGCCCGACGCTGTTCGGGGAAAAGATCGTTCTGCGTATCC
TCGATCCGGGTTCGGCCCAGATCGGCGTCGAAATGCTGGGATTCGAACCGGAGCAGCGGGACAATTTCCTGCGTGCCCTG
GAAAAACCCTACGGCATGATTCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCGTCACTCTCTACACCGGCCTGAACCT
GCTCAATCGCGTCGAAGCCAACATCTCCACGGTGGAGGACCCGGTCGAAATCACGGTTCCGGGGATCAATCAGGTCAACG
TCAACTACAAGACCGGCCTCACGTTCGCCGAGGCGCTGCGCGCATTTCTGCGCCAGGACCCCGACATCATCATGGTGGGC
GAGATCCGCGACCTGGAGACCGCCGAGATCGCCGTCAAGGCGGCCCAGACCGGCCATCTGGTGCTTTCGACCCTGCATAC
CAACGATGCCCCTCAGACCTTGAACCGTCTGATGCAGATGGGCATCCCGGCGTTCAACATCGCCTCCTCCGTCCTCCTGA
TCATGGCGCAGCGGCTGGCCCGCAGGCTTTGTCCGCACTGCAAGCGGGAGGAGAAGCTGCCGCCCGAGGTTCTGCTCGCG
TCCGGATTCCGGGAGGAAGATATCGGCAATTTCACGCTTTACGGACCGGGGGGTTGCGAGCAGTGCGTGAAAGGCTACAA
GGGGCGGGTCGGCATCTACCAGGTCATGCCGATTTCGGAGGAAATCAACCGCATCATCCTGGAAGGCGGGCACGTGATGG
CCCTGACCGAGCAGGCGCATGCAGAAGGCATCGCCGATCTGAGGGAGTCCGGCTTGAAGAAAGTGAGGGCGGGCATCACC
AGCCTCGAGGAAATCGACCGCGTGACGAGGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.174

100

0.566
  pilB Acinetobacter baylyi ADP1

52.941

100

0.536
  pilB Acinetobacter baumannii D1279779

51.832

100

0.52
  pilB Vibrio cholerae strain A1552

48.85

98.949

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

47.865

98.424

0.471
  pilF Neisseria gonorrhoeae MS11

47.34

98.774

0.468
  pilB Vibrio campbellii strain DS40M4

46.536

98.599

0.459
  pilF Thermus thermophilus HB27

38.313

99.65

0.382
  pilB/pilB1 Synechocystis sp. PCC 6803

38.779

97.548

0.378