Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KW115_RS13370 Genome accession   NZ_CP079095
Coordinates   2749362..2750240 (+) Length   292 a.a.
NCBI ID   WP_218806197.1    Uniprot ID   -
Organism   Methylococcus sp. Mc7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2744362..2755240
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KW115_RS13360 (KW115_13360) pilB 2746417..2748132 (+) 1716 WP_218806195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KW115_RS13365 (KW115_13365) pilC 2748137..2749351 (+) 1215 WP_218806196.1 type II secretion system F family protein Machinery gene
  KW115_RS13370 (KW115_13370) pilD 2749362..2750240 (+) 879 WP_218806197.1 A24 family peptidase Machinery gene
  KW115_RS13375 (KW115_13375) coaE 2750248..2750844 (+) 597 WP_218806198.1 dephospho-CoA kinase -
  KW115_RS13380 (KW115_13380) zapD 2750946..2751713 (+) 768 WP_218806199.1 cell division protein ZapD -
  KW115_RS13385 (KW115_13385) yacG 2751724..2751939 (+) 216 WP_218806200.1 DNA gyrase inhibitor YacG -
  KW115_RS13390 (KW115_13390) cysG 2751950..2753377 (-) 1428 WP_218806201.1 siroheme synthase CysG -
  KW115_RS13395 (KW115_13395) - 2753425..2753655 (+) 231 WP_218806202.1 sulfurtransferase TusA family protein -
  KW115_RS13400 (KW115_13400) - 2753664..2754326 (-) 663 WP_218806203.1 HAD family phosphatase -
  KW115_RS13405 (KW115_13405) - 2754517..2755092 (+) 576 WP_218806204.1 RNA pyrophosphohydrolase -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 32018.73 Da        Isoelectric Point: 6.6198

>NTDB_id=587638 KW115_RS13370 WP_218806197.1 2749362..2750240(+) (pilD) [Methylococcus sp. Mc7]
MNDFILFPEGPALIAICGLFGLIIGSFLNVVIHRLPLMMERAWRRECREFLEPGSEPPSGEPEYNLWRPGSQCPHCRAPV
RFWQNVPLLSYLWLRGRCAACGAAISRRYPLVEMLTGVLFATVAWHFGASWQTLAASGLTAGLIALAFIDLDHLVLPDDI
TLPLLWLGLLLNAQGFFCSLETALYGAVTGYLLLWTVHRGFLLLTGREGMGFGDFKLLAMLGAWMGWPMLPVIILFSSIS
GAVIGSIALAVGGKERDTPIPFGPFLAAGGWIALLWGNVLNDAYWRWASVGG

Nucleotide


Download         Length: 879 bp        

>NTDB_id=587638 KW115_RS13370 WP_218806197.1 2749362..2750240(+) (pilD) [Methylococcus sp. Mc7]
TTGAACGACTTTATTTTGTTTCCGGAGGGTCCAGCGCTGATCGCCATCTGCGGCCTGTTCGGACTGATCATCGGCAGTTT
TCTCAACGTGGTCATCCATCGCCTGCCGCTCATGATGGAGCGGGCATGGCGCAGGGAATGCCGGGAATTTCTCGAGCCCG
GCAGCGAGCCGCCTTCCGGGGAGCCCGAATACAACCTGTGGAGGCCGGGATCGCAGTGTCCGCATTGCCGGGCGCCGGTC
CGGTTCTGGCAGAATGTCCCGCTGCTCAGCTACCTGTGGCTTCGCGGCCGTTGCGCCGCCTGCGGCGCCGCGATCTCCCG
GCGTTATCCTCTGGTCGAGATGCTCACGGGCGTGCTGTTCGCAACGGTGGCCTGGCATTTCGGCGCGTCCTGGCAAACCC
TGGCCGCGTCCGGCCTGACGGCGGGCCTGATCGCCCTGGCTTTCATCGACCTCGACCATCTTGTGCTCCCGGACGACATC
ACCCTCCCCCTGCTTTGGCTGGGCCTGCTGCTGAACGCCCAGGGCTTCTTCTGCAGCCTGGAGACGGCGCTGTACGGCGC
CGTAACCGGCTATCTCCTCCTGTGGACGGTGCATCGCGGTTTTCTGCTGCTGACCGGACGCGAGGGGATGGGGTTCGGCG
ACTTCAAGCTGCTCGCGATGCTGGGTGCCTGGATGGGCTGGCCGATGCTGCCGGTGATCATCCTGTTCTCATCGATCTCC
GGGGCGGTGATCGGTTCGATTGCGCTGGCGGTGGGCGGAAAGGAGAGGGATACCCCGATTCCGTTCGGGCCGTTTCTGGC
GGCTGGCGGCTGGATCGCCCTGCTGTGGGGAAACGTGCTCAACGACGCCTACTGGCGCTGGGCCAGTGTCGGAGGTTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-127)

Type IV leader peptidase family (Peptidase_A24)

(140-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.747

93.493

0.493
  pilD Vibrio campbellii strain DS40M4

48.763

96.918

0.473
  pilD Neisseria gonorrhoeae MS11

49.281

95.205

0.469
  pilD Acinetobacter nosocomialis M2

46.503

97.945

0.455
  pilD Acinetobacter baumannii D1279779

46.154

97.945

0.452