Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EGT68_RS15180 Genome accession   NZ_CP078042
Coordinates   3193822..3194997 (+) Length   391 a.a.
NCBI ID   WP_125272396.1    Uniprot ID   -
Organism   Acinetobacter junii strain AJ_068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3188822..3199997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT68_RS15155 (EGT68_015155) - 3189485..3189958 (-) 474 WP_125272398.1 hypothetical protein -
  EGT68_RS15160 (EGT68_015160) guaD 3190144..3191469 (+) 1326 WP_125272397.1 guanine deaminase -
  EGT68_RS15165 (EGT68_015165) hpt 3191686..3192213 (+) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  EGT68_RS15170 (EGT68_015170) - 3192443..3192694 (+) 252 WP_004949988.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  EGT68_RS15175 (EGT68_015175) - 3192744..3193442 (-) 699 WP_039047176.1 DsbC family protein -
  EGT68_RS15180 (EGT68_015180) pilU 3193822..3194997 (+) 1176 WP_125272396.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EGT68_RS15185 (EGT68_015185) mnmE 3195053..3196408 (-) 1356 WP_125272405.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  EGT68_RS15190 (EGT68_015190) yidC 3196500..3198257 (-) 1758 WP_125272395.1 membrane protein insertase YidC -
  EGT68_RS15195 (EGT68_015195) yidD 3198263..3198583 (-) 321 WP_125272394.1 membrane protein insertion efficiency factor YidD -
  EGT68_RS15200 (EGT68_015200) rnpA 3198593..3198988 (-) 396 WP_004950005.1 ribonuclease P protein component -
  EGT68_RS15205 (EGT68_015205) rpmH 3199017..3199151 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44304.79 Da        Isoelectric Point: 6.2749

>NTDB_id=586490 EGT68_RS15180 WP_125272396.1 3193822..3194997(+) (pilU) [Acinetobacter junii strain AJ_068]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHHEVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=586490 EGT68_RS15180 WP_125272396.1 3193822..3194997(+) (pilU) [Acinetobacter junii strain AJ_068]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGCTACAGGGTTTAATGCGCCCTTTAGGACAACAAGCGCTTA
CCGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTTTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAACGTCCCGAATGTTTCACGGTTTCGGGTCAATGTATTCAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACATCTGAAATTCCAACCTTTCAAAAACTAAAACTACCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GTGGTTTGGTACTGGTGGTTGGCGGAACAGGGTCAGGGAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCA
TGAGGTTGGAGTTGACTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTGCGAACTCAAGATGGACAAGGTCGTCGAGCTGCGATTGAAA
TCCTCCTCAATACGCCATTGATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGTGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTGTATAACGAAGGTGCAATTAGTTACGATGAAGCTTT
GCGTAACGCCGACTCTGTCAATGAATTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCCGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAAGAAGTTGAAGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.923

93.606

0.542
  pilU Acinetobacter baylyi ADP1

55.331

88.747

0.491
  pilU Vibrio cholerae strain A1552

52.312

88.491

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.985

85.678

0.368
  pilT Acinetobacter nosocomialis M2

43.865

83.376

0.366
  pilT Acinetobacter baumannii D1279779

43.865

83.376

0.366
  pilT Acinetobacter baumannii strain A118

43.865

83.376

0.366
  pilT Acinetobacter baylyi ADP1

42.262

85.934

0.363