Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EGK58_RS01720 Genome accession   NZ_CP078027
Coordinates   394859..396568 (-) Length   569 a.a.
NCBI ID   WP_125278905.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain AV_175     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 389859..401568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGK58_RS01690 (EGK58_001690) - 389917..390240 (+) 324 WP_125278910.1 pyrimidine/purine nucleoside phosphorylase -
  EGK58_RS01695 (EGK58_001695) rlmB 390383..391132 (+) 750 WP_125278909.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  EGK58_RS01700 (EGK58_001700) - 391176..392093 (+) 918 WP_125278908.1 DMT family transporter -
  EGK58_RS01705 (EGK58_001705) coaE 392132..392737 (-) 606 WP_104425599.1 dephospho-CoA kinase -
  EGK58_RS01710 (EGK58_001710) pilD 392739..393599 (-) 861 WP_125278907.1 prepilin peptidase Machinery gene
  EGK58_RS01715 (EGK58_001715) pilC 393599..394828 (-) 1230 WP_125278906.1 type II secretion system F family protein Machinery gene
  EGK58_RS01720 (EGK58_001720) pilB 394859..396568 (-) 1710 WP_125278905.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGK58_RS01725 (EGK58_001725) tpiA 396847..397638 (+) 792 WP_125278904.1 triose-phosphate isomerase -
  EGK58_RS01730 (EGK58_001730) secG 397652..397981 (+) 330 WP_125278903.1 preprotein translocase subunit SecG -
  EGK58_RS01760 (EGK58_001760) rimP 398945..399469 (+) 525 WP_125278902.1 ribosome maturation factor RimP -
  EGK58_RS01765 (EGK58_001765) nusA 399505..400989 (+) 1485 WP_004787201.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63436.49 Da        Isoelectric Point: 4.7970

>NTDB_id=586404 EGK58_RS01720 WP_125278905.1 394859..396568(-) (pilB) [Acinetobacter variabilis strain AV_175]
MTALQGSPRFTGFIRRLVDEGVLSAEDMRTALANAKQEKTDIVAYLVSHHNLSPTTIAETISVEFGEPLFDIGAYDPGQI
LREDIDEKLITKHRILPIYKNANILYVATSNPTNIEATDAIRFATKLNIETVIVEHNKLEKLIEQNFTEESTFDFDDDFD
LDVDVDTADPTKEEDEKDKGEEAPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIATPPLQLANRLASR
LKVMSQMDISEKRVPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLQGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKKPADIPQQSLLEMGFT
ESDLQSPEFQIYEPVGCNECREGYKGRVGIYEVMKVTPEISRIIMEDGNAIEIAEASARAGFNNLRRSGLVKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=586404 EGK58_RS01720 WP_125278905.1 394859..396568(-) (pilB) [Acinetobacter variabilis strain AV_175]
ATGACAGCATTGCAGGGGTCACCAAGATTTACGGGGTTTATTCGTCGCCTAGTCGATGAAGGGGTATTATCAGCTGAAGA
TATGCGGACTGCTTTAGCCAATGCCAAACAGGAAAAAACAGATATCGTGGCCTATTTGGTCAGTCATCATAATCTTTCCC
CTACTACCATTGCTGAAACGATCTCAGTAGAATTTGGTGAGCCTCTATTTGATATTGGTGCTTATGATCCGGGACAAATC
CTGCGTGAAGATATTGATGAAAAGCTGATTACCAAGCATCGTATTCTGCCAATTTATAAAAACGCCAATATTCTTTATGT
CGCCACGAGCAATCCGACTAATATTGAAGCAACGGATGCCATCCGCTTTGCGACTAAACTGAATATTGAAACCGTTATTG
TTGAACACAATAAACTCGAGAAACTGATCGAGCAAAACTTTACTGAAGAAAGTACTTTTGATTTTGATGATGATTTCGAT
CTGGATGTTGATGTCGACACAGCCGATCCAACGAAAGAAGAAGATGAAAAAGACAAGGGTGAAGAAGCCCCGATTGTTAA
ATATATTAATAAATTACTGATTGATGCCATTCGTATGGGCGCTTCGGATCTACATTTTGAACCTTATGAAAAAATGTATC
GGGTGCGCTATCGTGTCGATGGGGTTTTACGTCAGATTGCAACCCCGCCCCTACAACTGGCAAACCGTTTGGCGTCGCGT
TTGAAAGTCATGTCGCAGATGGACATTTCTGAGAAACGCGTGCCTCAAGACGGCCGTATTAAATTAAAGCTCTCAAAAAA
CAAAGCTATCGACTTCCGTGTTAACTCACTCCCTACCCTGTTTGGCGAAAAACTGGTTCTACGTATTCTAGATCCGTCTA
GTGCCATGCTAGGAATTGATGCTTTAGGTTATGAACCTGAGCAGAAAGAGTTATTCATGGAAGCCCTAGATAAACCGCAA
GGGATGTTACTGATTACCGGTCCTACCGGTTCCGGTAAAACCGTATCACTTTATACGGGCTTAAACATCTTAAACCGCGA
GGACACCAATATTTCTACTGCGGAAGATCCAGTCGAGATTAATCTGCAAGGGATTAATCAGGTGAACGTGAATAATAAAG
TTGGCCTGACGTTTGCAGCTGCTTTAAAATCCTTCTTGCGTCAGGATCCTGATATTGTCATGGTCGGTGAGATCCGTGAT
CTGGAAACAGCTGAAATTGCAATTAAAGCAGCCCAAACAGGTCATATGGTGATGTCAACCCTGCACACCAACAGTGCTCC
GGAAACTCTGACCCGTTTACGTAATATGGGCGTCCCTTCTTTCAATATCGCGACCTCGGTCAATCTAGTGATTGCGCAGC
GTCTGGCCCGTCGCTTGTGTTCACAATGTAAAAAGCCGGCTGATATTCCTCAGCAAAGTTTGCTGGAAATGGGCTTTACC
GAATCAGATTTACAAAGCCCTGAATTTCAGATTTATGAGCCAGTCGGCTGTAATGAATGCCGTGAAGGATATAAGGGCCG
GGTCGGTATTTACGAAGTCATGAAAGTCACACCTGAAATTTCAAGAATTATTATGGAAGATGGCAATGCCATTGAAATTG
CTGAAGCTTCCGCGCGTGCAGGATTTAACAATTTACGCCGATCAGGGTTAGTGAAGGTTATGCAGGGGGTGACTTCTTTA
CAGGAAGTCAATCGTGTAACCAGCGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.053

100

0.812
  pilB Acinetobacter baylyi ADP1

76.491

100

0.766
  pilB Legionella pneumophila strain ERS1305867

50.707

99.473

0.504
  pilF Neisseria gonorrhoeae MS11

49.02

98.594

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

49.013

97.891

0.48
  pilB Vibrio campbellii strain DS40M4

48.644

97.188

0.473
  pilB Vibrio cholerae strain A1552

50.385

91.388

0.46
  pilF Thermus thermophilus HB27

38.313

100

0.383
  pilB/pilB1 Synechocystis sp. PCC 6803

34.258

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.138

97.012

0.36