Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EGT69_RS16410 Genome accession   NZ_CP078018
Coordinates   3430819..3431994 (+) Length   391 a.a.
NCBI ID   WP_004914541.1    Uniprot ID   S7Y0P3
Organism   Acinetobacter junii strain AJ_351     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3425819..3436994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT69_RS16385 (EGT69_016385) - 3426483..3426956 (-) 474 WP_004914520.1 hypothetical protein -
  EGT69_RS16390 (EGT69_016390) guaD 3427142..3428467 (+) 1326 WP_125297808.1 guanine deaminase -
  EGT69_RS16395 (EGT69_016395) hpt 3428684..3429211 (+) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  EGT69_RS16400 (EGT69_016400) - 3429440..3429691 (+) 252 WP_004949988.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  EGT69_RS16405 (EGT69_016405) - 3429741..3430439 (-) 699 WP_004949990.1 DsbC family protein -
  EGT69_RS16410 (EGT69_016410) pilU 3430819..3431994 (+) 1176 WP_004914541.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EGT69_RS16415 (EGT69_016415) mnmE 3432050..3433405 (-) 1356 WP_026057420.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  EGT69_RS16420 (EGT69_016420) yidC 3433496..3435253 (-) 1758 WP_125297807.1 membrane protein insertase YidC -
  EGT69_RS16425 (EGT69_016425) yidD 3435259..3435579 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  EGT69_RS16430 (EGT69_016430) rnpA 3435589..3435984 (-) 396 WP_004950005.1 ribonuclease P protein component -
  EGT69_RS16435 (EGT69_016435) rpmH 3436013..3436147 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44323.84 Da        Isoelectric Point: 6.3220

>NTDB_id=586395 EGT69_RS16410 WP_004914541.1 3430819..3431994(+) (pilU) [Acinetobacter junii strain AJ_351]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=586395 EGT69_RS16410 WP_004914541.1 3430819..3431994(+) (pilU) [Acinetobacter junii strain AJ_351]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGTTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CTGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCAAGGTTTCGGGTCAATGTTTTCAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACGTCTGAAATTCCAACCTTTCAAAAACTAAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GAGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGAAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTACGAACTCAAGATGGTCAAGGACGTCGTGCCGCGATTGAAA
TCCTACTCAATACGCCATTGATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGCGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTATATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAGTTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCGGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAGGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y0P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.197

93.606

0.545
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.491

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366