Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EGT69_RS02145 Genome accession   NZ_CP078018
Coordinates   462433..464157 (-) Length   574 a.a.
NCBI ID   WP_125297382.1    Uniprot ID   -
Organism   Acinetobacter junii strain AJ_351     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457433..469157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGT69_RS02115 (EGT69_002115) - 457515..457841 (+) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -
  EGT69_RS02120 (EGT69_002120) rlmB 458033..458782 (+) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  EGT69_RS02125 (EGT69_002125) - 458813..459724 (+) 912 WP_125297384.1 DMT family transporter -
  EGT69_RS02130 (EGT69_002130) coaE 459721..460320 (-) 600 WP_125297383.1 dephospho-CoA kinase -
  EGT69_RS18300 (EGT69_002135) pilD 460322..461182 (-) 861 WP_004916856.1 prepilin peptidase Machinery gene
  EGT69_RS02140 (EGT69_002140) pilC 461182..462408 (-) 1227 WP_004916853.1 type II secretion system F family protein Machinery gene
  EGT69_RS02145 (EGT69_002145) pilB 462433..464157 (-) 1725 WP_125297382.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGT69_RS02150 (EGT69_002150) tpiA 464503..465294 (+) 792 WP_005403576.1 triose-phosphate isomerase -
  EGT69_RS02155 (EGT69_002155) secG 465307..465636 (+) 330 WP_004916847.1 preprotein translocase subunit SecG -
  EGT69_RS02175 (EGT69_002175) rimP 466259..466783 (+) 525 WP_004916844.1 ribosome maturation factor RimP -
  EGT69_RS02180 (EGT69_002180) nusA 466821..468305 (+) 1485 WP_004916840.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63784.92 Da        Isoelectric Point: 5.0021

>NTDB_id=586366 EGT69_RS02145 WP_125297382.1 462433..464157(-) (pilB) [Acinetobacter junii strain AJ_351]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIEQQFGDSGSFDFWENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSETKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LEKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVQSFNIATSVNLVIAQRLARRLCSSCKQPIDIPKQSLL
EMGFTEQDLTTPGFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFDNLRRSGLKKVMQ
GITSLQEVNRVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=586366 EGT69_RS02145 WP_125297382.1 462433..464157(-) (pilB) [Acinetobacter junii strain AJ_351]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTTATACAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAACACAATAAACTCGAAAAACTAATTGAACAGCAGTTTGGAGATAGTGGCAGTTTCGATTTTTGGGAAAATGACGAC
TTTGATTTAGATAATGTTGATGTTGCAACGACTGATACAAACAACACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCCCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGCGCCTCTGACTTACATTTTGAACCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGAGTACTCAGACAAATTGCTAATCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTGAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TTTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGAAAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACAGGTTTAAA
TATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTAGAAACAGCTGAAATTGCTATCAAAGCAGCCCAAACAGGTCACATGGTGATGTCGACTTTGCA
CACCAATAGCGCTCCAGAAACACTCACACGTTTACGTAATATGGGAGTTCAGTCTTTCAATATTGCGACTTCGGTTAATC
TAGTAATTGCTCAGCGGTTAGCTCGCCGCCTATGTTCTTCATGCAAACAACCTATCGATATTCCTAAACAAAGCCTTCTA
GAAATGGGTTTCACCGAACAAGACTTAACCACCCCAGGTTTCCAAATATTTCAACCTGTAGGCTGTGATGCTTGTCGTGA
AGGATATAAAGGACGTGTCGGTATTTATGAAGTGATGAAAGTGACACCAGAGATTTCCAAGATTATTATGGAAGATGGGA
ATGCACTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTCGATAATTTACGTCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.556

100

0.808
  pilB Acinetobacter baylyi ADP1

76.174

100

0.763
  pilB Legionella pneumophila strain ERS1305867

52.23

93.728

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

49.731

97.038

0.483
  pilF Neisseria gonorrhoeae MS11

50.091

95.296

0.477
  pilB Vibrio cholerae strain A1552

48.556

96.516

0.469
  pilB Vibrio campbellii strain DS40M4

50.87

90.07

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

34.572

100

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.837

92.857

0.361
  pilF Thermus thermophilus HB27

36.637

98.432

0.361